%0 Journal Article %A Gao, Haichun %A Obraztova, Anna %A Stewart, Nathan %A Popa, Radu %A Fredrickson, James K. %A Tiedje, James M. %A Nealson, Kenneth H. %A Zhou, Jizhong %T Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean %D 2006 %J International Journal of Systematic and Evolutionary Microbiology, %V 56 %N 8 %P 1911-1916 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.64354-0 %I Microbiology Society, %X A novel marine bacterial strain, PV-4T, isolated from a microbial mat located at a hydrothermal vent of Loihi Seamount in the Pacific Ocean, has been characterized. This micro-organism is orangey in colour, Gram-negative, polarly flagellated, facultatively anaerobic and psychrotolerant (temperature range, 0–42 °C). No growth was observed with nitrate, nitrite, DMSO or thiosulfate as the electron acceptor and lactate as the electron donor. The major fatty acid detected in strain PV-4T was iso-C15 : 0. Strain PV-4T had ubiquinones consisting mainly of Q-7 and Q-8, and possessed menaquinone MK-7. The DNA G+C content of the strain was 53.8 mol% and the genome size was about 4.5 Mbp. Phylogenetic analyses based on 16S rRNA gene sequences placed PV-4T within the genus Shewanella. PV-4T exhibited 16S rRNA gene sequence similarity levels of 99.6 and 97.5 %, respectively, with respect to the type strains of Shewanella aquimarina and Shewanella marisflavi. DNA from strain PV-4T showed low mean levels of relatedness to the DNAs of S. aquimarina (50.5 %) and S. marisflavi (8.5 %). On the basis of phylogenetic and phenotypic characteristics, the bacterium was classified in the genus Shewanella within a distinct novel species, for which the name Shewanella loihica sp. nov. is proposed. The type strain is PV-4T (=ATCC BAA-1088T=DSM 17748T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64354-0