RT Journal Article SR Electronic(1) A1 Gao, Haichun A1 Obraztova, Anna A1 Stewart, Nathan A1 Popa, Radu A1 Fredrickson, James K. A1 Tiedje, James M. A1 Nealson, Kenneth H. A1 Zhou, JizhongYR 2006 T1 Shewanella loihica sp. nov., isolated from iron-rich microbial mats in the Pacific Ocean JF International Journal of Systematic and Evolutionary Microbiology, VO 56 IS 8 SP 1911 OP 1916 DO https://doi.org/10.1099/ijs.0.64354-0 PB Microbiology Society, SN 1466-5034, AB A novel marine bacterial strain, PV-4T, isolated from a microbial mat located at a hydrothermal vent of Loihi Seamount in the Pacific Ocean, has been characterized. This micro-organism is orangey in colour, Gram-negative, polarly flagellated, facultatively anaerobic and psychrotolerant (temperature range, 0–42 °C). No growth was observed with nitrate, nitrite, DMSO or thiosulfate as the electron acceptor and lactate as the electron donor. The major fatty acid detected in strain PV-4T was iso-C15 : 0. Strain PV-4T had ubiquinones consisting mainly of Q-7 and Q-8, and possessed menaquinone MK-7. The DNA G+C content of the strain was 53.8 mol% and the genome size was about 4.5 Mbp. Phylogenetic analyses based on 16S rRNA gene sequences placed PV-4T within the genus Shewanella. PV-4T exhibited 16S rRNA gene sequence similarity levels of 99.6 and 97.5 %, respectively, with respect to the type strains of Shewanella aquimarina and Shewanella marisflavi. DNA from strain PV-4T showed low mean levels of relatedness to the DNAs of S. aquimarina (50.5 %) and S. marisflavi (8.5 %). On the basis of phylogenetic and phenotypic characteristics, the bacterium was classified in the genus Shewanella within a distinct novel species, for which the name Shewanella loihica sp. nov. is proposed. The type strain is PV-4T (=ATCC BAA-1088T=DSM 17748T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64354-0