1887

Abstract

A novel marine bacterial strain, PV-4, isolated from a microbial mat located at a hydrothermal vent of Loihi Seamount in the Pacific Ocean, has been characterized. This micro-organism is orangey in colour, Gram-negative, polarly flagellated, facultatively anaerobic and psychrotolerant (temperature range, 0–42 °C). No growth was observed with nitrate, nitrite, DMSO or thiosulfate as the electron acceptor and lactate as the electron donor. The major fatty acid detected in strain PV-4 was iso-C. Strain PV-4 had ubiquinones consisting mainly of Q-7 and Q-8, and possessed menaquinone MK-7. The DNA G+C content of the strain was 53.8 mol% and the genome size was about 4.5 Mbp. Phylogenetic analyses based on 16S rRNA gene sequences placed PV-4 within the genus . PV-4 exhibited 16S rRNA gene sequence similarity levels of 99.6 and 97.5 %, respectively, with respect to the type strains of and . DNA from strain PV-4 showed low mean levels of relatedness to the DNAs of (50.5 %) and (8.5 %). On the basis of phylogenetic and phenotypic characteristics, the bacterium was classified in the genus within a distinct novel species, for which the name sp. nov. is proposed. The type strain is PV-4 (=ATCC BAA-1088=DSM 17748).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64354-0
2006-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/8/1911.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64354-0&mimeType=html&fmt=ahah

References

  1. Bowman J., McCammon S., Nichols D., Skerratt J., Rea S., Nichols P., McMeekin T. 1997; Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20 : 5 ω 3) and grow anaerobically by dissimilatory Fe(III) reduction. Int J Syst Bacteriol 47:1040–1047 [CrossRef]
    [Google Scholar]
  2. Bozal N., Montes M. J., Tudela E., Jimenez F., Guinea J. 2002; Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. Int J Syst Evol Microbiol 52:195–205
    [Google Scholar]
  3. Brettar I., Christen R., Höfle M. G. 2002; Shewanella denitrificans sp. nov., a vigorously denitrifying bacterium isolated from the oxic–anoxic interface of the Gotland Deep in the central Baltic Sea. Int J Syst Evol Microbiol 52:2211–2217 [CrossRef]
    [Google Scholar]
  4. Coyne V. E., Pillidge C. J., Sledjeski D. D., Hori H., Ortizconde B. A., Muir D. G., Weiner R. M., Colwell R. R. 1989; Reclassification of Alteromonas colwelliana to the genus Shewanella by DNA-DNA hybridization, serology and 5S ribosomal-RNA sequence data. Syst Appl Microbiol 12:275–279 [CrossRef]
    [Google Scholar]
  5. Gauthier G., Gauthier M., Christen R. 1995; Phylogenetic analysis of the genera Alteromonas , Shewanella , and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol 45:755–761 [CrossRef]
    [Google Scholar]
  6. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. 1998; Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44:1148–1153 [CrossRef]
    [Google Scholar]
  7. Ivanova E. P., Sawabe T., Gorshkova N. M., Svetashev V. I., Mikhailov V. V., Nicolau D. V., Christen R. 2001; Shewanella japonica sp. nov. Int J Syst Evol Microbiol 51:1027–1033 [CrossRef]
    [Google Scholar]
  8. Ivanova E. P., Sawabe T., Hayashi K., Gorshkova N. M., Zhukova N. V., Nedashkovskaya O. I., Mikhailov V. V., Nicolau D. V., Christen R. 2003a; Shewanella fidelis sp. nov., isolated from sediments and sea water. Int J Syst Evol Microbiol 53:577–582 [CrossRef]
    [Google Scholar]
  9. Ivanova E. P., Nedashkovskaya O. I., Zhukova N. V., Nicolau D. V., Christen R., Mikhailov V. V. 2003b; Shewanella waksmanii sp. nov., isolated from a sipuncula ( Phascolosoma japonicum ). Int J Syst Evol Microbiol 53:1471–1477 [CrossRef]
    [Google Scholar]
  10. Ivanova E. P., Gorshkova N. M., Bowman J. P., Lysenko A. M., Zhukova N. V., Sergeev A. F., Mikhailov V. V., Nicolau D. V. 2004a; Shewanella pacifica sp. nov., a polyunsaturated fatty acid-producing bacterium isolated from sea water. Int J Syst Evol Microbiol 54:1083–1087 [CrossRef]
    [Google Scholar]
  11. Ivanova E. P., Nedashkovskaya O. I., Sawabe T., Zhukova N. V., Frolova G. M., Nicolau D. V., Mikhailov V. V., Bowman J. P. 2004b; Shewanella affinis sp. nov., isolated from marine invertebrates. Int J Syst Evol Microbiol 54:1089–1093 [CrossRef]
    [Google Scholar]
  12. Ivanova E. P., Flavier S., Christen R. 2004c; Phylogenetic relationships among marine Alteromonas -like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam.nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 54:1773–1788 [CrossRef]
    [Google Scholar]
  13. Kato C., Li L., Nogi Y., Nakamura Y., Tamaoka J., Horikoshi K. 1998; Extremely barophilic bacteria isolated from the Mariana Trench, Challenger Deep, at a depth of 11,000 meters. Appl Environ Microbiol 64:1510–1513
    [Google Scholar]
  14. Kostka J. E., Stucki J. W., Nealson K. H., Wu J. 1996; Reduction of structural Fe(III) in smectite by a pure culture of Shewanella putrefaciens strain MR-1. Clays Clay Miner 44:522–529 [CrossRef]
    [Google Scholar]
  15. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  16. Leonardo M. R., Moser D. P., Barbieri E., Brantner C. A., MacGregor B. J., Paster B. J., Stackebrandt E., Nealson K. H. 1999; Shewanella pealeana sp. nov., a member of the microbial community associated with the accessory nidamental gland of the squid Loligo pealei . Int J Syst Bacteriol 49:1341–1351 [CrossRef]
    [Google Scholar]
  17. MacDonell M. T., Colwell R. R. 1985; Phylogeny of the Vibrionaceae , and recommendation for 2 new genera, Listonella and Shewanella . Syst Appl Microbiol 6:171–182 [CrossRef]
    [Google Scholar]
  18. Makemson J. C., Fulayfil N. R., Landry W., Van Ert L. M., Wimpee C. F., Widder E. A., Case J. F. 1997; Shewanella woodyi sp. nov., an exclusively respiratory luminous bacterium isolated from the Alboran Sea. Int J Syst Bacteriol 47:1034–1039 [CrossRef]
    [Google Scholar]
  19. Myers C. R., Nealson K. H. 1988; Bacterial manganese reduction and growth with manganese oxide as the sole electron-acceptor. Science 240:1319–1321 [CrossRef]
    [Google Scholar]
  20. Nogi Y., Kato C., Horikoshi K. 1998; Taxonomic studies of deep-sea barophilic Shewanella strains and description of Shewanella violacea sp. nov. Arch Microbiol 170:331–338 [CrossRef]
    [Google Scholar]
  21. Nozue H., Hayashi T., Hashimoto Y., Ezaki T., Hamasaki K., Ohwada K., Terawaki Y. 1992; Isolation and characterization of Shewanella alga from human clinical specimens and emendation of the description of S. alga Simidu et al. 1990, 335. Int J Syst Bacteriol 42:628–634 [CrossRef]
    [Google Scholar]
  22. Petrovskis E. A., Vogel T. M., Adriaens P. 1994; Effects of electron acceptors and donors on transformation of tetrachloromethane by Shewanella putrefaciens MR-1. FEMS Microbiol Lett 121:357–363 [CrossRef]
    [Google Scholar]
  23. Roh Y., Gao H., Vali H. 9 other authors 2006; Metal reduction and iron biomineralization by a psychrotolerant Fe(III)-reducing bacterium, Shewanella sp. PV-4. Appl Environ Microbiol 72:3236–3244 [CrossRef]
    [Google Scholar]
  24. Russell N. J., Nichols D. S. 1999; Polyunsaturated fatty acids in marine bacteria – a dogma rewritten. Microbiology 145:767–779 [CrossRef]
    [Google Scholar]
  25. Satomi M., Oikawa H., Yano Y. 2003; Shewanella marinintestina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from sea-animal intestines. Int J Syst Evol Microbiol 53:491–499 [CrossRef]
    [Google Scholar]
  26. Satomi M., Fonnesbech Vogel B., Gram L., Venkateswaran K. 2006; Shewanella hafniensis sp. nov. and Shewanella morhuae sp. nov., isolated from marine fish of the Baltic Sea. Int J Syst Evol Microbiol 56:243–249 [CrossRef]
    [Google Scholar]
  27. Semple K. M., Westlake D. W. S. 1987; Characterization of iron-reducing Alteromonas putrefaciens strains from oil-field fluids. Can J Microbiol 33:366–371 [CrossRef]
    [Google Scholar]
  28. Skerratt J. H., Bowman J. P., Nichols P. D. 2002; Shewanella olleyana sp. nov., a marine species isolated from a temperate estuary which produces high levels of polyunsaturated fatty acids. Int J Syst Evol Microbiol 52:2101–2106 [CrossRef]
    [Google Scholar]
  29. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt F., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  30. Stackebrandt E., Goebel B. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  31. Stapleton R. D. Jr, Sabree Z. L., Palumbo A. V., Moyer C. L., Devol A. H., Roh Y., Zhou J. 2005; Metal reduction at cold temperatures by Shewanella isolates from various marine environments. Aquat Microb Ecol 38:81–91 [CrossRef]
    [Google Scholar]
  32. Toffin L., Bidault A., Pignet P., Tindall B. J., Slobodkin A., Kato C., Prieur D. 2004; Shewanella profunda sp. nov., isolated from deep marine sediment of the Nankai Trough. Int J Syst Evol Microbiol 54:1943–1949 [CrossRef]
    [Google Scholar]
  33. Venkateswaran K., Dollhopf M. E., Aller R., Stackebrandt E., Nealson K. H. 1998; Shewanella amazonensis sp. nov., a novel metal-reducing facultative anaerobe from Amazonian shelf muds. Int J Syst Bacteriol 48:965–972 [CrossRef]
    [Google Scholar]
  34. Venkateswaran K., Moser D. P., Dollhopf M. E. 10 other authors 1999; Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. Int J Syst Bacteriol 49:705–724 [CrossRef]
    [Google Scholar]
  35. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  36. Weiner R. M., Coyne V. E., Brayton P., West P., Raiken S. F. 1988; Alteromonas colwelliana sp. nov., an isolate from oyster habitats. Int J Syst Bacteriol 38:240–244 [CrossRef]
    [Google Scholar]
  37. Xu M. Y., Guo J., Cen Y. H., Zhong X. Y., Cao W., Sun G. P. 2005; Shewanella decolorationis sp. nov., a dye-decolorizing bacterium isolated from activated sludge of a waste-water treatment plant. Int J Syst Evol Microbiol 55:363–368 [CrossRef]
    [Google Scholar]
  38. Yoon J. H., Kang K. H., Oh T. K., Park Y. H. 2004a; Shewanella gaetbuli sp. nov., a slight halophile isolated from a tidal flat in Korea. Int J Syst Evol Microbiol 54:487–491 [CrossRef]
    [Google Scholar]
  39. Yoon J. H., Yeo S. H., Kim I. G., Oh T. K. 2004b; Shewanella marisflavi sp. nov. and Shewanella aquimarina sp. nov., slightly halophilic organisms isolated from sea water of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54:2347–2352 [CrossRef]
    [Google Scholar]
  40. Zhao J. S., Greer C. W., Thiboutot S., Ampleman G., Hawari J. 2004; Biodegradation of the nitramine explosives hexahydro-1,3,5-trinitro-1,3,5-triazine and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine in cold marine sediment under anaerobic and oligotrophic conditions. Can J Microbiol 50:91–96 [CrossRef]
    [Google Scholar]
  41. Zhao J.-S., Manno D., Beaulieu C., Paquet L., Hawari J. 2005; Shewanella sediminis sp. nov., a novel Na+-requiring and hexahydro-1,3,5-trinitro-1,3,5-triazine-degrading bacterium from marine sediment. Int J Syst Evol Microbiol 55:1511–1520 [CrossRef]
    [Google Scholar]
  42. Zhao J.-S., Manno D., Beaulieu C., Paquet L., Hawari J. 2006; Shewanella halifaxensis sp. nov., a novel obligately respiratory and denitrifying psychrophile. Int J Syst Evol Microbiol 56:205–212 [CrossRef]
    [Google Scholar]
  43. Zhou J.-Z., Bruns M. A., Tiedje J. M. 1996; DNA recovery from soils of diverse composition. Appl Environ Microbiol 62:316–322
    [Google Scholar]
  44. Ziemke F., Hofle M. G., Lalucat J., Rossello-Mora R. 1998; Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48:179–186 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64354-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64354-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error