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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 57, Issue 7

sp. nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens Free

Abstract

Five coagulase-negative, novobiocin-susceptible staphylococcal strains were isolated from human blood cultures in different German and Belgian medical facilities. A novel species, ‘’ was proposed recently to accommodate two of these strains (B3117 and A6664), although the name was not validly published. All five strains belonged to the genus because they were non-motile, Gram-positive, catalase-positive cocci with peptidoglycan type (A3 type -lys–gly–-Ser–Gly), menaquinone pattern (MK-7, MK-6 and MK-8) and major cellular fatty acids (ai-C, ai-C and i-C) that corresponded to those of staphylococci. Phenotypically, the isolates most closely resembled subsp. and , but they could be distinguished from these species by physiological tests and chemotaxonomic investigations. The results of DNA–DNA hybridization, chemotaxonomic investigations and 16S rRNA gene and RNA polymerase B gene () sequence analysis enabled strains B3117, K6999, 229 and 230 to be differentiated genotypically and phenotypically from known species, indicating that these isolates are representatives of a novel species. The name sp. nov. is proposed for this novel species, with strain B3117 (=CIP 107711=CCUG 51270) as the type strain. Due to differences in the results of physiological and chemotaxonomic investigations and DNA–DNA hybridization data, strain A6664 was not included in the description of the novel species.

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Keyword(s): CoNS, coagulase-negative staphylococci
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2007-07-01
2024-04-16
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References

  1. Bannerman T. L. 2003; Staphylococcus , Micrococcus , and other catalase-positive cocci that grow aerobically. In Manual of Clinical Microbiology , 8th edn. pp  384–404 Edited by Murray P. R., Baron E. J., Jorgensen J. H., Pfaller M. A., Yolken R. H. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  2. Becker K., Schumann P., Wüllenweber J., Schulte M., Weil H. P., Stackebrandt E., Peters G., von Eiff C. 2002; Kytococcus schroeteri sp. nov., a novel Gram-positive actinobacterium isolated from a human clinical source. Int J Syst Evol Microbiol 52:1609–1614 [CrossRef]
     [Google Scholar]
  3. Breitkopf C., Hammel D., Scheld H. H., Peters G., Becker K. 2005; Impact of a molecular approach to improve the microbiological diagnosis of infective heart valve endocarditis. Circulation 111:1415–1421 [CrossRef]
     [Google Scholar]
  4. Bruce J. 1996; Automated system rapidly identifies and characterizes microorganisms in food. Food Technol 50:77–81
     [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
     [Google Scholar]
  6. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
     [Google Scholar]
  7. Cowan S. T., Steel K. J. 1974 Manual for the Identification of Medical Bacteria , 2nd edn. Cambridge: Cambridge University Press;
     [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
     [Google Scholar]
  9. DSMZ 2001 Catalogue of Strains, 7th edn. Germany: DSMZ;
     [Google Scholar]
  10. Freney J., Kloos W. E., Hajek V., Webster J. A., Bes M., Brun Y., Vernozy-Rozand C. 1999; Recommended minimal standards for description of new staphylococcal species. Subcommittee on the taxonomy of staphylococci and streptococci of the International Committee on Systematic Bacteriology. Int J Syst Bacteriol 49:489–502 [CrossRef]
     [Google Scholar]
  11. Götz F., Bannerman T., Schleifer K. H. 2006; The Genera Staphylococcus and Macrococcus . In The Prokaryotes vol. 4, Bacteria: Firmicutes , Cyanobacteria pp  5–75 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
     [Google Scholar]
  12. Groth I., Schumann P., Martin K., Schuetze B., Augsten K., Kramer I., Stackebrandt E. 1999; Ornithinicoccus hortensis gen. nov., sp. nov., a soil actinomycete which contains l-ornithine. Int J Syst Bacteriol 49:1717–1724 [CrossRef]
     [Google Scholar]
  13. Huebner J., Goldmann D. A. 1999; Coagulase-negative staphylococci: role as pathogens. Annu Rev Med 50:223–236 [CrossRef]
     [Google Scholar]
  14. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
     [Google Scholar]
  15. Kloos W., Tornabene T. G., Schleifer K. H. 1974; Isolation and characterization of micrococci from human skin, including two new species: Micrococcus lylae and Micrococcus kristinae . Int J Syst Bacteriol 24:79–101 [CrossRef]
     [Google Scholar]
  16. Kotilainen P., Huovinen P., Eerola E. 1991; Application of gas-liquid chromatographic analysis of cellular fatty acids for species identification and typing of coagulase-negative staphylococci. J Clin Microbiol 29:315–322
     [Google Scholar]
  17. Ludwig W., Strunk O., Klugbauer S., Klugbauer N., Weizenegger N., Neumaier J., Bachleitner M., Schleifer K.-H. 1998; Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19:554–568 [CrossRef]
     [Google Scholar]
  18. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
     [Google Scholar]
  19. Mellmann A., Becker K., von Eiff C., Keckevoet U., Schumann P., Harmsen D. 2006; Sequencing and staphylococci identification. Emerg Infect Dis 12:333–336 [CrossRef]
     [Google Scholar]
  20. Miller L. T. 1982; Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16:584–586
     [Google Scholar]
  21. Pagano L., Tacconelli E., Tumbarello M., Laurenti L., Ortu-La Barbera E., Antinori A., Caponera S., Cauda R., Leone G. 1997; Bacteremia in patients with hematological malignancies. Analysis of risk factors, etiological agents and prognostic indicators. Haematologica 82:415–419
     [Google Scholar]
  22. Pessoa-Silva C. L., Miyasaki C. H., de Almeida M. F., Kopelman B. I., Raggio R. L., Wey S. B. 2001; Neonatal late-onset bloodstream infection: attributable mortality, excess of length of stay and risk factors. Eur J Epidemiol 17:715–720 [CrossRef]
     [Google Scholar]
  23. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
     [Google Scholar]
  24. Stackebrandt E., Koch C., Gvozdiak O., Schumann P. 1995; Taxonomic dissection of the genus Micrococcus : Kocuria gen.nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45:682–692 [CrossRef]
     [Google Scholar]
  25. Trülzsch K., Rinder H., Trcek J., Bader L., Wilhelm U., Heesemann J. 2002; ‘ Staphylococcus pettenkoferi ’, a novel staphylococcal species isolated from clinical specimens. Diagn Microbiol Infect Dis 43:175–182 [CrossRef]
     [Google Scholar]
  26. von Eiff C., Jansen B., Kohnen W., Becker K. 2005; Infections associated with medical devices: pathogenesis, management and prophylaxis. Drugs 65:179–214 [CrossRef]
     [Google Scholar]
  27. von Eiff C., Arciola C. R., Montanaro L., Becker K., Campoccia D. 2006; Emerging Staphylococcus species as new pathogens in implant infections. Int J Artif Organs 29:360–367
     [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
     [Google Scholar]
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Staphylococcus pettenkoferi sp. nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens
Int J Syst Evol Microbiol 57, 1543 (2007); https://doi.org/10.1099/ijs.0.64381-0
/content/journal/ijsem/10.1099/ijs.0.64381-0
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