Natrinema ejinorense sp. nov., isolated from a saline lake in Inner Mongolia, China Castillo, A. M. and Gutiérrez, M. C. and Kamekura, M. and Xue, Y. and Ma, Y. and Cowan, D. A. and Jones, B. E. and Grant, W. D. and Ventosa, A.,, 56, 2683-2687 (2006), doi = https://doi.org/10.1099/ijs.0.64421-0, publicationName = Microbiology Society, issn = 1466-5026, abstract= A Gram-negative, non-motile, neutrophilic, pleomorphic and extremely halophilic archaeon, strain EJ-57T, was isolated from saline Lake Ejinor in Inner Mongolia, China. Strain EJ-57T was able to grow at 25–50 °C, required at least 1.8 M NaCl for growth (optimum at 3.4 M NaCl) and grew over a pH range from 6.0 to 8.5 (optimum at pH 7.0). Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. Analysis of the almost complete 16S rRNA gene sequence indicated that the isolate represented a member of the genus Natrinema in the family Halobacteriaceae. Strain EJ-57T was most closely related to Natrinema versiforme JCM 10478T (96.2 % sequence similarity), Natrinema pallidum NCIMB 777T (95.9 % sequence similarity), Natrinema altunense JCM 12890T (95.8 % sequence similarity) and Natrinema pellirubrum NCIMB 786T (95.5 % sequence similarity). However, DNA–DNA hybridization experiments showed that strain EJ-57T was not related to these species, with levels of DNA–DNA relatedness equal to or below 39 %. The major polar lipids of the isolate were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and the disulfated glycolipid S2-DGA-1. The G+C content of the genomic DNA was 64.7 mol%. Comparative analysis of phenotypic characteristics between strain EJ-57T and recognized Natrinema species supported the conclusion that EJ-57T represents a novel species within this genus, for which the name Natrinema ejinorense sp. nov. is proposed. The type strain is EJ-57T (=CECT 7144T=JCM 13890T=CGMCC 1.6202T)., language=, type=