1887

Abstract

Analysis of 16S rRNA gene sequences is one of the most common methods for investigating the phylogeny and taxonomy of bacteria. However, several studies have indicated that the 16S rRNA gene does not distinguish between certain species. We therefore selected for phylogenetic analysis an alternative marker, , encoding gyrase subunit B. The aim of this investigation was to examine the applicability of gene fragments (~1100 bp) for the phylogenetic study of 16 species and a total of 33 strains included in this study. Based on the sequenced fragments, a phylogenetic tree was obtained that contained two distinct clusters, with gastric species forming one cluster and enterohepatic species the other. The only exception was the gastric species , which clustered with the enterohepatic species. The calculated similarity matrix revealed the highest interspecies similarity between and (89 %) and the lowest similarity between and (60 %). The DNA G+C content of the sequenced fragments was ⩽40 mol% in enterohepatic species and >46 mol% in gastric species, excluding and , with G+C contents of 34 and 42 mol%, respectively. In summary, the gene fragments provided superior resolution and reliability to the 16S rRNA gene for differentiating between closely related species. A further outcome of this study was achieved by designing gene-based species-specific PCR primers for the identification of .

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2007-03-01
2024-03-28
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