1887

Abstract

An actinobacterial strain containing demethylmenaquinone DMK-9(H) as the diagnostic isoprenoid quinone was isolated from a tidal flat sediment sample, from South Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain JC2054 represents a distinct phyletic line within the suborder of the order . The closest phylogenetic neighbour was , with 94.7 % 16S rRNA gene sequence similarity. The novel isolate was strictly aerobic and slightly halophilic, with optimum growth occurring in 2–4 % (w/v) NaCl. Cells were non-motile, non-sporulating and rod-shaped. The peptidoglycan type was of the A-type of cross-linkage. -ornithine was the diamino acid and -glutamate represented the N-terminus of the interpeptide bridge. The predominant fatty acids were anteiso-branched and straight-chain fatty acids. The major polar lipids were phosphatidylinositol, diphosphatidylglycerol and an unknown phospholipid. The menaquinone composition of was determined to be MK-10(H), MK-9(H) and MK-8(H) in the ratio 56 : 2 : 1. On the basis of the polyphasic evidence presented in this study, it is proposed that strain JC2054 should be classified as representing a novel genus and species of the suborder , with the name gen. nov., sp. nov. The type strain is JC2054 (=IMSNU 14027=KCTC 9919=JCM 12123). In addition, it was clear from the phylogenetic analysis and chemotaxonomic data that does not belong to the genus or any other recognized genera. Therefore, should be reclassified as representing a novel genus, for which the name gen. nov., comb. nov. is proposed, with strain DSM 3133 (=ATCC 43279=CFBP 4259=CIP 103003=NBRC 15517=JCM 9966=LMG 16154) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64525-0
2007-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/1/151.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64525-0&mimeType=html&fmt=ahah

References

  1. Altenburger P., Kämpfer P., Schumann P., Vybiral D., Lubitz W., Busse H. J. 2002; Georgenia muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting. Int J Syst Evol Microbiol 52:875–881 [CrossRef]
    [Google Scholar]
  2. An D.-S., Im W.-T., Yang H.-C., Kang M. S., Kim K. K., Jin L., Kim M. K., Lee S.-T. 2005; Cellulomonas terrae sp. nov., a cellulolytic and xylanolytic bacterium isolated from soil. Int J Syst Evol Microbiol 55:1705–1709 [CrossRef]
    [Google Scholar]
  3. Bagnara C., Toci R., Gaudin C., Belaich J. P. 1985; Isolation and characterization of a cellulolytic microorganism, Cellulomonas fermentans sp. nov. Int J Syst Bacteriol 35:502–507 [CrossRef]
    [Google Scholar]
  4. Baumann L., Baumann P., Mandel M., Allen R. D. 1972; Taxonomy of aerobic marine eubacteria. J Bacteriol 110:402–429
    [Google Scholar]
  5. Brown J. M., Frazier R. P., Morey R. E., Steigerwalt A. G., Pellegrini G. J., Daneshvar M. I., Hollis D. G., McNeil M. M. 2005; Phenotypic and genetic characterization of clinical isolates of CDC coryneform group A-3: proposal of a new species of Cellulomonas , Cellulomonas denverensis sp. nov.. J Clin Microbiol 43:1732–1737 [CrossRef]
    [Google Scholar]
  6. Brown J. M., Steigerwalt A. G., Morey R. E., Daneshvar M. I., Romero L.-J., McNeil M. M. 2006; Characterization of clinical isolates previously identified as Oerskovia turbata : proposal of Cellulosimicrobium funkei sp. nov. and emended description of the genus Cellulosimicrobium . Int J Syst Evol Microbiol 56:801–804 [CrossRef]
    [Google Scholar]
  7. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  8. Collins M. D. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp  265–309 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  9. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  10. Elberson M. A., Malekzadeh F., Yazdi M. T., Kameranpour N., Noori-Daloii M. R., Matte M. H., Shahamat M., Colwell R. R., Sowers K. R. 2000; Cellulomonas persica sp. nov. and Cellulomonas iranensis sp. nov., mesophilic cellulose-degrading bacteria isolated from forest soils. Int J Syst Evol Microbiol 50:993–996 [CrossRef]
    [Google Scholar]
  11. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  12. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  13. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  14. Fitch W. M., Margoliash E. 1967; Construction of phylogenetic trees. Science 155:279–284 [CrossRef]
    [Google Scholar]
  15. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  16. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  17. Groth I., Schumann P., Martin K., Schuetze B., Augsten K., Kramer I., Stackebrandt E. 1999; Ornithinicoccus hortensis gen. nov., sp. nov., a soil actinomycete which contains l-ornithine. Int J Syst Bacteriol 49:1717–1724 [CrossRef]
    [Google Scholar]
  18. Groth I., Schumann P., Schütze B., Gonzalez J. M., Laiz L., Saiz-Jimenez C., Stackebrandt E. 2005; Isoptericola hypogeus sp. nov., isolated from the Roman catacomb of Domitilla. Int J Syst Evol Microbiol 55:1715–1719 [CrossRef]
    [Google Scholar]
  19. Hanada S., Liu W.-T., Shintani T., Kamagata Y., Nakamura K. 2002; Tetrasphaera elongata sp. nov., a polyphosphate-accumulating bacterium isolated from activated sludge. Int J Syst Evol Microbiol 52:883–887 [CrossRef]
    [Google Scholar]
  20. Huang Y., Dai X., He L., Wang Y.-N., Wang B.-J., Liu Z., Liu S.-J. 2005; Sanguibacter marinus sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 55:1755–1758 [CrossRef]
    [Google Scholar]
  21. Ishikawa T., Yokota A. 2006; Reclassification of Arthrobacter duodecadis Lochhead 1958 as Tetrasphaera duodecadis comb. nov. and emended description of the genus Tetrasphaera . Int J Syst Evol Microbiol 56:1369–1373 [CrossRef]
    [Google Scholar]
  22. Jeon Y.-S., Chung H., Park S., Hur I., Lee J.-H., Chun J. 2005; jPHYDIT: a JAVA-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21:3171–3173 [CrossRef]
    [Google Scholar]
  23. Jones B. E., Grant W. D., Duckworth A. W., Schumann P., Weiss N., Stackebrandt E. 2005; Cellulomonas bogoriensis sp. nov., an alkaliphilic cellulomonad. Int J Syst Evol Microbiol 55:1711–1714 [CrossRef]
    [Google Scholar]
  24. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol  3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  25. Kim B. S., Oh H. M., Kang H., Park S. S., Chun J. 2004; Remarkable bacterial diversity in the tidal flat sediment as revealed by 16S rDNA analysis. J Microbiol Biotechnol 14:205–211
    [Google Scholar]
  26. Kim B. S., Oh H. M., Kang H., Chun J. 2005; Archaeal diversity in tidal flat sediment as revealed by 16S rDNA analysis. J Microbiol 43:144–151
    [Google Scholar]
  27. Mackenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N -heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160
    [Google Scholar]
  28. Maszenan A. M., Seviour R. J., Patel B. K. C., Schumann P., Burghardt J., Tokiwa Y., Stratton H. M. 2000; Three isolates of novel polyphosphate-accumulating Gram-positive cocci, obtained from activated sludge, belong to a new genus, Tetrasphaera gen. nov., and description of two new species, Tetrasphaera japonica sp.nov. and Tetrasphaera australiensis sp. nov.. Int J Syst Evol Microbiol 50:593–603 [CrossRef]
    [Google Scholar]
  29. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  30. Park Y.-D., Baik K. S., Yi H., Bae K. S., Chun J. 2005; Pseudoalteromonas byunsanensis sp. nov., isolated from tidal flat sediment in Korea. Int J Syst Evol Microbiol 55:2519–2523 [CrossRef]
    [Google Scholar]
  31. Prauser H., Schumann P., Rainey F. A., Kroppenstedt R. M., Stackebrandt E. 1997; Terracoccus luteus gen. nov., sp. nov., an ll-diaminopimelic acid-containing coccoid actinomycete from soil. Int J Syst Bacteriol 47:1218–1224 [CrossRef]
    [Google Scholar]
  32. Pukall R., Schumann P., Schütte C., Gols R., Dicke M. 2006; Acaricomes phytoseiuli gen. nov., sp. nov., isolated from the predatory mite Phytoseiulus persimilis . Int J Syst Evol Microbiol 56:465–469 [CrossRef]
    [Google Scholar]
  33. Rivas R., Trujillo M. E., Mateos P. F., Martínez-Molina E., Velázquez E. 2004; Cellulomonas xylanilytica sp. nov., a cellulolytic and xylanolytic bacterium isolated from a decayed elm tree. Int J Syst Evol Microbiol 54:533–536 [CrossRef]
    [Google Scholar]
  34. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  35. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  36. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  37. Schleifer K. H., Seidl P. H. 1985; Chemical composition and structure of murein. In Chemical Methods in Bacterial Systematics pp  201–219 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  38. Schumann P., Weiss N., Stackebrandt E. 2001; Reclassification of Cellulomonas cellulans (Stackebrandt and Keddie 1986) as Cellulosimicrobium cellulans gen. nov., comb. nov.. Int J Syst Evol Microbiol 51:1007–1010 [CrossRef]
    [Google Scholar]
  39. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  40. Stackebrandt E., Schumann P. 2000; Introduction to the taxonomy of actinobacteria. In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community , release 3.3 Edited by Dworkin M. New York: Springer; http://link.springer-ny.com/link/service/books/10125/
    [Google Scholar]
  41. Stackebrandt E., Schumann P., Prauser H. 2002 The Family Cellulomonadaceae . In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community , release 3.0 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; http://link.springer-ny.com/link/service/books/10125/
    [Google Scholar]
  42. Stackebrandt E., Schumann P., Cui X.-L. 2004; Reclassification of Cellulosimicrobium variabile Bakalidou et al. , 2002 as Isoptericola variabilis gen. nov., comb. nov.. Int J Syst Evol Microbiol 54:685–688 [CrossRef]
    [Google Scholar]
  43. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium : new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942
    [Google Scholar]
  44. Swofford D. L. 1998 paup*: Phylogenetic Analysis Using Parsimony (*and other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  45. Uchida K., Aida K. 1984; An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30:131–134 [CrossRef]
    [Google Scholar]
  46. Yassin A. F., Haggenei B., Budzikiewicz H., Schaal K. P. 1993; Fatty acid and polar lipid composition of the genus Amycolatopsis : application of fast atom bombardment-mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43:414–420 [CrossRef]
    [Google Scholar]
  47. Yi H., Chun J. 2006; Thalassobius aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 44:171–176
    [Google Scholar]
  48. Zhang Y.-Q., Schumann P., Li W.-J., Chen G.-Z., Tian X.-P., Stackebrandt E., Xu L.-H., Jiang C.-L. 2005; Isoptericola halotolerans sp. nov., a novel actinobacterium isolated from saline soil from Qinghai Province, north-west China. Int J Syst Evol Microbiol 55:1867–1870 [CrossRef]
    [Google Scholar]
  49. Zobell C. E. 1941; Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4:42–75
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64525-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64525-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error