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Volume 57, Issue 2

Nitrogen-fixing and cellulose-producing sp. nov., isolated from Kombucha tea Free

Abstract

A few members of the family are cellulose-producers, while only six members fix nitrogen. Bacterial strain RG3, isolated from Kombucha tea, displays both of these characteristics. A high bootstrap value in the 16S rRNA gene sequence-based phylogenetic analysis supported the position of this strain within the genus , with LMG 1527 as its nearest neighbour (99.1 % sequence similarity). It could utilize ethanol, fructose, arabinose, glycerol, sorbitol and mannitol, but not galactose or xylose, as sole sources of carbon. Single amino acids such as -alanine, -cysteine and -threonine served as carbon and nitrogen sources for growth of strain RG3. Strain RG3 produced cellulose in both nitrogen-free broth and enriched medium. The ubiquinone present was Q-10 and the DNA base composition was 55.8 mol% G+C. It exhibited low values of 5.2–27.77 % DNA–DNA relatedness to the type strains of related gluconacetobacters, which placed it within a separate taxon, for which the name sp. nov. is proposed, with the type strain RG3 (=LMG 23726=MTCC 6913).

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2007-02-01
2024-04-19
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Nitrogen-fixing and cellulose-producing Gluconacetobacter kombuchae sp. nov., isolated from Kombucha tea
Int J Syst Evol Microbiol 57, 353 (2007); https://doi.org/10.1099/ijs.0.64638-0
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