1887

Abstract

Four Gram-negative, facultatively anaerobic, non-spore-forming isolates of coccoid rods were obtained from fruit powder and investigated in a polyphasic taxonomic study. Comparative 16S rRNA gene sequence analysis allocated the isolates to the family . Their phylogenetic position within the family was confirmed by sequence analysis and as the highest sequence similarities were obtained with , and , the isolates clearly belong to the genus . Biochemical data revealed that the isolates can be separated into two distinct groups that represent two novel species, as confirmed by DNA–DNA hybridizations. The two novel species can be differentiated from their nearest neighbours by the following characteristics: the utilization of sucrose, -sorbitol, putrescine and mucate, the hydrolysis of aesculin and a negative result in the Voges–Proskauer reaction. It is therefore proposed that these novel isolates are classified as sp. nov. (type strain 508/05=LMG 23730=DSM 18397) and sp. nov. (type strain 513/05=LMG 23732=DSM 18396).

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2007-04-01
2024-03-29
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