1887

Abstract

An aerobic, Gram-negative, non-motile, yellow-pigmented bacterium, strain KMM 3046, was isolated from a deep-sea brittle star from the Fiji Sea and was subjected to a polyphasic taxonomic analysis. Strain KMM 3046 grew at 5–32 °C and in the presence of 1–12 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone and 3-OH i16 : 0, 3-OH i17 : 0 and 3-OH a17 : 0 as the major fatty acids. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KMM 3046 forms a distinct evolutionary lineage within the family (phylum ), displaying 92.3–91.9 % sequence similarity with respect to species. On the basis of the phenotypic and phylogenetic data, strain KMM 3046 represents a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is KMM 3046 (=NRIC 0684=JCM 14121).

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2007-03-01
2024-03-28
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References

  1. Barbeyron T., L'Haridon S., Corre E., Kloareg B., Potin P. 2001; Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [ Cytophaga ] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov.. Int J Syst Evol Microbiol 51:985–997 [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y. 2003 An introduction to the family Flavobacteriaceae . In The Prokaryotes, An Evolving Electronic Resource for the Microbiological Community , release 3.15 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; http://link.springer-ny.com/link/service/books/10125/
    [Google Scholar]
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  5. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917 [CrossRef]
    [Google Scholar]
  6. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surface of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
    [Google Scholar]
  7. Bowman J. P., Nichols D. S. 2002; Aequorivita gen. nov., a member of the family Flavobacteriaceae isolated from terrestrial and marine Antarctic habitats. Int J Syst Evol Microbiol 52:1533–1541 [CrossRef]
    [Google Scholar]
  8. Bowman J. P., Nichols C. M., Gibson J. A. E. 2003; Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53:1343–1355 [CrossRef]
    [Google Scholar]
  9. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [CrossRef]
    [Google Scholar]
  10. Cho J. C., Giovannoni S. J. 2003; Croceibacter atlanticus gen. nov., sp. nov., a novel marine bacterium in the family Flavobacteriaceae . Syst Appl Microbiol 26:76–83 [CrossRef]
    [Google Scholar]
  11. Dobson S. J., Colwell R. R., McMeekin T. A., Franzmann P. D. 1993; Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43:77–83 [CrossRef]
    [Google Scholar]
  12. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [CrossRef]
    [Google Scholar]
  13. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5.1. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  14. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  15. Ivanova E. P., Bowman J. P., Christen R., Zhukova N. V., Lysenko A. M., Gorshkova N. M., Mitik-Dineva N., Sergeev A. F., Mikhailov V. V. 2006; Salegentibacter flavus sp. nov. Int J Syst Evol Microbiol 56:583–586 [CrossRef]
    [Google Scholar]
  16. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  17. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  18. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  19. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  20. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp.nov. and Flavobacterium xanthum sp. nov., nom.rev. and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50:1055–1063 [CrossRef]
    [Google Scholar]
  21. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Rohde M., Zhukova N. V., Falsen E., Frolova G. M., Mikhailov V. V. other authors 2003; Mesonia algae gen. nov., sp. nov. a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm. . Int J Syst Evol Microbiol 53:1967–1971 [CrossRef]
    [Google Scholar]
  22. Nedashkovskaya O. I., Suzuki M., Vancanneyt M., Cleenwerck I., Zhukova N. V., Vysotskii M. V., Mikhailov V. V., Swings J. 2004; Salegentibacter holothuriorum sp. nov., isolated from the edible holothurian Apostichopus japonicus . Int J Syst Evol Microbiol 54:1107–1110 [CrossRef]
    [Google Scholar]
  23. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Mikhailov V. V., Bae K. S., Kim I. S. 2005a; Salegentibacter mishustinae sp. nov, isolated from the sea urchin Strongylocentrotus intermedius . Int J Syst Evol Microbiol 55:235–237 [CrossRef]
    [Google Scholar]
  24. Nedashkovskaya O. I., Vancanneyt M., Dawyndt P., Engelbeen K., Vandemeulebroecke K., Cleenwerck I., Hoste B., Mergaert J., Tan T. L. other authors 2005b; Reclassification of [ Cytophaga ] marinoflava Reichenbach 1989 as Leeuwenhoekiella marinoflava gen.nov., comb. nov. and description of Leeuwenhoekiella aequorea sp. nov.. Int J Syst Evol Microbiol 55:1033–1038 [CrossRef]
    [Google Scholar]
  25. Nedashkovskaya O. I., Kim S. B., Vancanneyt M., Shin D. S., Lysenko A. M., Shevchenko L. S., Krasokhin V. B., Mikhailov V. V., Swings J., Bae K. S. 2006; Salegentibacter agarivorans sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the sponge Artemisina sp. Int J Syst Evol Microbiol 56:883–887 [CrossRef]
    [Google Scholar]
  26. Nichols D. S., Nichols P. D., McMeekin T. A. 1993; Polyunsaturated fatty acids in Antarctic bacteria. Antarct Sci 5:149–160
    [Google Scholar]
  27. Owen J., Hill L. R., Lapage S. P. 1969; Determination of DNA base composition from melting profiles in dilute buffers. Biopolymers 7:503–516 [CrossRef]
    [Google Scholar]
  28. Pearson W., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  29. Pinhassi J., Bowman J. P., Nedashkovskaya O. I., Lekunberri I., Gomez-Consarnau L., Pedrós-Alió C. 2006; Leeuwenhoekiella blandensis sp. nov., a genome-sequenced marine member of the family Flavobacteriaceae . Int J Syst Evol Microbiol 56:1489–1493 [CrossRef]
    [Google Scholar]
  30. Reichenbach H. 1992 The order Cytophagales. In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications , 2nd edn. pp  3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  31. Romanenko L. A., Uchino M., Falsen E., Zhukova N. V., Mikhailov V. V., Uchimura T. 2003; Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from the deep sea of the Pacific. Int J Syst Evol Microbiol 53:1973–1977 [CrossRef]
    [Google Scholar]
  32. Romanenko L. A., Schumann P., Rohde M., Mikhailov V. V., Stackebrandt E. 2004; Reinekea marinisedimentorum gen. nov., sp. nov., a novel gammaproteobacterium from marine coastal sediments. Int J Syst Evol Microbiol 54:669–673 [CrossRef]
    [Google Scholar]
  33. Rowe N. J., Tunstall J., Galbraith L., Wilkinson S. G. 2000; Lipid composition of [ Pseudomonas ] echinoides : transfer to the genus Sphingomonas . Microbiology 146:3007–3012
    [Google Scholar]
  34. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  35. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [CrossRef]
    [Google Scholar]
  36. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  37. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga -like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb.nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp.nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:1639–1652 [CrossRef]
    [Google Scholar]
  38. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  39. Vaskovsky V. E., Terekhova T. A. 1979; HPTLC of phospholipid mixtures containing phosphatidylglycerol. J High Resolut Chromatogr 2:671–672 [CrossRef]
    [Google Scholar]
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