RT Journal Article SR Electronic(1) A1 Wang, Qian-fu A1 Li, Wei A1 Yang, Hai A1 Liu, Yan-li A1 Cao, Hai-hua A1 Dornmayr-Pfaffenhuemer, Marion A1 Stan-Lotter, Helga A1 Guo, Guang-qinYR 2007 T1 Halococcus qingdaonensis sp. nov., a halophilic archaeon isolated from a crude sea-salt sample JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 3 SP 600 OP 604 DO https://doi.org/10.1099/ijs.0.64673-0 PB Microbiology Society, SN 1466-5034, AB A Gram-negative, extremely halophilic, coccoid archaeal strain, CM5T, was isolated from a crude sea-salt sample collected near Qingdao, China. The organism grew optimally at 35–40 °C and pH 6.0 in the presence of 20 % (w/v) NaCl. Its colonies were red in colour and it could use glucose as a sole carbon source for growth. The 16S rRNA gene sequence of CM5T was most closely related to those of Halococcus species. Its pattern of antibiotic susceptibility was similar to those of other described Halococcus species. Biochemical tests revealed no sign of H2S production or gelatin liquefaction. The main polar lipids of strain CM5T were phosphatidylglycerol, phosphatidylglycerol methylphosphate and sulfated diglycosyl diether. No phosphatidylglycerol sulfate was present. The DNA G+C content of strain CM5T was 61.2 mol% and it gave DNA–DNA reassociation values of 33.7, 57.1 and 29.6 %, respectively, with Halococcus salifodinae DSM 8989T, Halococcus dombrowskii DSM 14522T and Halococcus morrhuae ATCC 17082T. Based on its morphological and chemotaxonomic properties and phylogenetic analysis of 16S rRNA gene sequence data, we propose that CM5T should be classified within a novel species, Halococcus qingdaonensis sp. nov., with strain CM5T (=CGMCC 1.4243T=JCM 13587T) as the type strain., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64673-0