1887

Abstract

A Gram-negative, extremely halophilic, coccoid archaeal strain, CM5, was isolated from a crude sea-salt sample collected near Qingdao, China. The organism grew optimally at 35–40 °C and pH 6.0 in the presence of 20 % (w/v) NaCl. Its colonies were red in colour and it could use glucose as a sole carbon source for growth. The 16S rRNA gene sequence of CM5 was most closely related to those of species. Its pattern of antibiotic susceptibility was similar to those of other described species. Biochemical tests revealed no sign of HS production or gelatin liquefaction. The main polar lipids of strain CM5 were phosphatidylglycerol, phosphatidylglycerol methylphosphate and sulfated diglycosyl diether. No phosphatidylglycerol sulfate was present. The DNA G+C content of strain CM5 was 61.2 mol% and it gave DNA–DNA reassociation values of 33.7, 57.1 and 29.6 %, respectively, with DSM 8989, DSM 14522 and ATCC 17082. Based on its morphological and chemotaxonomic properties and phylogenetic analysis of 16S rRNA gene sequence data, we propose that CM5 should be classified within a novel species, sp. nov., with strain CM5 (=CGMCC 1.4243=JCM 13587) as the type strain.

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2007-03-01
2024-03-29
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References

  1. De Ley L., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  2. Denner E. B. M., McGenity T. J., Busse H.-J., Grant W. D., Wanner G., Stan-Lotter H. 1994; Halococcus salifodinae sp. nov., an archaeal isolate from an Austrian salt mine. Int J Syst Bacteriol 44:774–780 [CrossRef]
    [Google Scholar]
  3. Dussault H. P. 1955; An improved technique for staining red halophilic bacteria. J Bacteriol 70:484–485
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Garrity G. M., Bell J. A., Lilburn T. G. 2004; Taxonomic outline of the prokaryotes. In Bergey's Manual of Systematic Bacteriology , 2nd edn, release 5.0. New York: Springer; http://141.150.157.80/bergeysoutline/main.htm
    [Google Scholar]
  6. Gibbons N. E. 1974; Family V. Halobacteriaceae fam. nov. In Bergey's Manual of Determinative Bacteriology , 8th edn. pp  269–273 Edited by Buchanan R. G., Gibbons N. E. Baltimore: Williams & Wilkins;
    [Google Scholar]
  7. Goh F., Leuko S., Allen M. A., Bowman J. P., Kamekura M., Neilan B. A., Burns B. P. 2006; Halococcus hamelinensis sp. nov., a novel halophilic archaeon isolated from stromatolites in Shark Bay, Australia. Int J Syst Evol Microbiol 56:1323–1329 [CrossRef]
    [Google Scholar]
  8. Grant W. D., Larsen H. 1989; Group III. Extremely halophilic archaeobacteria. Order Halobacteriales ord. nov. In Bergey's Manual of Systematic Bacteriology vol. 3 pp  2216–2219 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  9. Gutierrez M. C., Ventosa A., Ruiz-Berraquero F. 1989; DNA-DNA homology studies among strains of Haloferax and other halobacteria. Curr Microbiol 18:253–256 [CrossRef]
    [Google Scholar]
  10. Gutierrez M. C., Ventosa A., Ruiz-Berraquero F. 1990; Deoxyribonucleic acid relatedness among species of Haloarcula and other halobacteria. Biochem Cell Biol 68:106–110 [CrossRef]
    [Google Scholar]
  11. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  12. Jahnke K.-D. 1992; basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:621–627
    [Google Scholar]
  13. Kumar S., Tamura K., Jakobson I.-B., Nei M. 2001; mega2: molecular evolution analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  14. Kumar S., Tamura K., Jakobson I.-B., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  15. Kushner D. J., Kamekura M. 1988; Physiology of halophilic eubacteria. In Halobacteria vol. 1 pp  109–140 Edited by Rodriguez-Valera F. Boca Raton, FL: CRC Press;
    [Google Scholar]
  16. Larsen H. 1989; Genus IV. Halococcus Schoop 1935, 817AL . In Bergey's Manual of Systematic Bacteriology vol  3 pp  2228–2230 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  17. Mancinelli R. L., Hochstein L. I. 1986; The occurrence of denitrification in extremely halophilic bacteria. FEMS Microbiol Lett 35:55–58 [CrossRef]
    [Google Scholar]
  18. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  19. Montero C. G., Ventosa A., Rodriguez-Valera F., Kates M., Moldoveanu N., Ruiz-Berraquero F. 1989; Halococcus saccharolyticus sp. nov., a new species of extremely halophilic non-alkaliphilic cocci. Syst Appl Microbiol 12:167–171 [CrossRef]
    [Google Scholar]
  20. Oren A., Trüper H. G. 1990; Anaerobic growth of halophilic archaeobacteria by reduction of dimethylsulfoxide and trimethylamine N -oxide. FEMS Microbiol Lett 70:33–36 [CrossRef]
    [Google Scholar]
  21. Oren A., Ventosa A., Grant W. D. 1997; Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  22. Ross H. M., Grant W. D., Harris J. E. 1985; Lipids in archaeabacterial taxonomy. In Chemical Methods in Bacterial Systematics pp  289–299 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Seghal S. N., Gibbons N. E. 1960; Effect of some metal ions on the growth of Halobacterium cutirubrum . Can J Microbiol 6:165–169 [CrossRef]
    [Google Scholar]
  25. Skerman V. B. D., McGowan V., Sneath P. H. A. (editors) 1980; Approved lists of bacterial names. Int J Syst Bacteriol 30:225–420 [CrossRef]
    [Google Scholar]
  26. Spurr A. R. 1969; A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43 [CrossRef]
    [Google Scholar]
  27. Stan-Lotter H., McGenity T. J., Legat A., Denner E. B. M., Glaser K., Stetter K. O., Wanner G. 1999; Very similar strains of Halococcus salifodinae are found in geographically separated Permo-Triassic salt deposits. Microbiology 145:3565–3574
    [Google Scholar]
  28. Stan-Lotter H., Pfaffenhuemer M., Legat A., Busse H.-J., Radax C., Gruber C. 2002; Halococcus dombrowskii sp. nov., an archaeal isolate from a Permian alpine salt deposit. Int J Syst Evol Microbiol 52:1807–1814 [CrossRef]
    [Google Scholar]
  29. Thompson J. D., Gibson T. J., Plewniak K., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  30. Tian X. Y., Xu Y., Liu H. C. 1997; A new species of Natronobacterium . Wei Sheng Wu Xue Bao 37:1–6 (in Chinese)
    [Google Scholar]
  31. Tindall B. J. 1990; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
    [Google Scholar]
  32. Wainø M., Tindall B. J., Ingvorsen K. 2000; Halorhabdus utahensis gen nov., sp. nov an aerobic, extremely halophilic member of the Archaea from Great Salt Lake, Utah. Int J Syst Evol Microbiol 50183–190 [CrossRef]
    [Google Scholar]
  33. Wang Z., Xu Y., Zhou P. 2000; Taxonomy of a new species of haloalkalophilic archaeon. Wei Sheng Wu Xue Bao 40:115–120 (in Chinese)
    [Google Scholar]
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