1887

Abstract

A novel halophilic archaeon, strain TL6, was isolated from Telega Lake, a hypersaline environment in Prahova county, Romania. Strain TL6 was able to grow in media with a salt concentration of between 2.5 and 5.2 M, with optimum growth at a concentration of 3.5 M. The novel strain was able to grow at concentrations of 1 M MgCl or less, with an optimum of 0.4 M Mg. Growth of the novel strain occurred between pH 6.0 and 8.5, with an optimum of pH 7.0–7.5. The G+C content of the total DNA was 63.7 mol%. The 16S rRNA gene sequence of the novel strain was most closely related to species of the genus (97.3–99.3 % sequence similarity). The lipid profile of the novel strain corresponded to that of other species belonging to the genus . A comparative analysis of the phenotypic properties and DNA–DNA hybridization between the novel strain and other species of the genus strongly supported the conclusion that strain TL6 represents a novel species within this genus, for which the name sp. nov., is proposed. The type strain is TL6 (=JCM 13924=DSM 18310).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64674-0
2007-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/2/393.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64674-0&mimeType=html&fmt=ahah

References

  1. Asker D., Ohta Y. 2002; Haloferax alexandrinus sp. nov., an extremely halophilic canthaxanthin-producing archaeon from a solar saltern in Alexandria (Egypt). Int J Syst Evol Microbiol 52:729–738 [CrossRef]
    [Google Scholar]
  2. Castillo A. M., Gutiérrez M. C., Kamekura M., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. 2006a; Halovivax asiaticus gen. nov., sp. nov., a novel extremely halophilic archaeon isolated from Inner Mongolia, China. Int J Syst Evol Microbiol 56:765–770 [CrossRef]
    [Google Scholar]
  3. Castillo A. M., Gutiérrez M. C., Kamekura M., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. (2006b); Halostagnicola larsenii gen. nov., sp. nov., an extremely halophilic archaeon from a saline lake in Inner Mongolia, China. Int J Syst Evol Microbiol 56:1519–1524 [CrossRef]
    [Google Scholar]
  4. Elshahed M. S., Savage K. N., Oren A., Gutierrez M. C., Ventosa A., Krumholz L. R. 2004; Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring. Int J Syst Evol Microbiol 54:2275–2279 [CrossRef]
    [Google Scholar]
  5. Enache M., Teodosiu G., Faghi A. M., Dumitru L. 2000; Identification of halophilic Archaebacteria isolated from some Romanian salts lakes on the basis of lipids composition. Rev Roum Biol Ser Biol Vég 45:93–99
    [Google Scholar]
  6. Ezaki T., Hashimoto H., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  7. Grant W. D., Kamekura M., McGenity T. J., Ventosa A. 2001; The order Halobacteriales . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  294–334 Edited by Boone D. R., Castenholz R. W. New York: Springer;
    [Google Scholar]
  8. Gutierrez C., Gonzalez C. 1972; Method for simultaneous detection of proteinase and esterase activities in extremely halophilic bacteria. Appl Microbiol 24:516–517
    [Google Scholar]
  9. Gutierrez M. C., Kamekura M., Holmes M. L., Dyall-Smith M. L., Ventosa A. 2002; Taxonomic characterization of Haloferax sp.(“ H. alicantei ”) strain Aa 2.2: description of Haloferax lucentensis sp. nov. Extremophiles 6:479–483 [CrossRef]
    [Google Scholar]
  10. Hezayen F. F., Tindall B. J., Steinbuchel A., Rehm B. H. A. 2002; Characterization of a novel halophilic archaeon, Halobiforma haloterrestris gen. nov., sp. nov., and transfer of Natronobacterium nitratireducens to Halobiforma nitratireducens comb. nov. Int J Syst Evol Microbiol 52:2271–2280 [CrossRef]
    [Google Scholar]
  11. Holding A. J., Collee J. G. 1971; Routine Biochemical Tests. In Methods in Microbiology vol 6A pp  1–32 Edited by Norris J. R., Ribbons D. W. London and New York: Academic Press;
    [Google Scholar]
  12. Itoh T., Yamaguchi T., Zhou P., Takashina T. 2005; Natronolimnobius baerhuensis gen. nov., sp. nov., and Natronolimnobius innemongolicus sp. nov., novel haloalkaliphilic archaea isolated from soda lakes in Inner Mongolia, China. Extremophiles 9:111–116 [CrossRef]
    [Google Scholar]
  13. Juez G., Rodriguez-Valera F., Ventosa A., Kushner D. J. 1986; Haloarcula hispanica spec. nov. and Haloferax gibbonsii spec. nov., two new species of extremely halophilic archaebacteria. Syst Appl Microbiol 8:75–79 [CrossRef]
    [Google Scholar]
  14. Kamekura M. 1993; Lipids of extreme halophiles. In The Biology of Halophilic Bacteria pp  135–161 Edited by Vreeland R. H., Hochstein L. I. Boca Raton: CRC Press;
    [Google Scholar]
  15. Kamekura M., Mizuki T., Usami R., Youshida Y., Horikoshi K., Vreeland R. H. 2004; The potential use of signature bases from 16S rRNA gene sequences to aid the assignment of microbial strains to genera of halobacteria. In Halophilic Microorganisms pp  77–87 Edited by Ventosa A. Berlin, Heidelberg: Springer;
    [Google Scholar]
  16. Meseguer I., Rodriguez-Valera F. 1985; Production and purification of halocin H4. FEMS Microbiol Lett 28:177–182 [CrossRef]
    [Google Scholar]
  17. Mullakhanbhai M. F., Larsen H. 1975; Halobacterium volcanii spec. nov., a Dead Sea halobacterium with a moderate salt requirement. Arch Microbiol 104:207–214 [CrossRef]
    [Google Scholar]
  18. Oren A., Elevi R. V., Watanabe S., Ihara K., Corcelli A. 2002; Halomicrobium mukohataei gen. nov., comb. nov., and emended description of Halomicrobium mukohataei . Int J Syst Evol Microbiol 52:1831–1835 [CrossRef]
    [Google Scholar]
  19. Rodriguez-Valera F., Juez G., Kushner D. J. 1983; Halobacterium mediterranei spec. nov., a new carbohydrate-utilizing extreme halophile. Syst Appl Microbiol 4:369–381 [CrossRef]
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  21. Tamaoka J. 1994; Determination of DNA base composition. In Chemical Methods in Prokaryotic Systematics pp  463–470 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  23. Tindall B. J., Tomlinson G. A., Hochstein L. I. 1989; Transfer of Halobacterium denitrificans (Tomlinson, Jahnke, and Hochstein) to the genus Haloferax as Haloferax denitrificans comb. nov. Int J Syst Bacteriol 39:359–360 [CrossRef]
    [Google Scholar]
  24. Tomlinson G. A., Jahnke L. L., Hochstein L. I. 1986; Halobacterium denitrificans sp. nov., an extremely halophilic denitrifying bacterium. Int J Syst Bacteriol 36:66–70 [CrossRef]
    [Google Scholar]
  25. Torreblanca M., Rodriguez-Valera F., Juez G., Ventosa A., Kamekura M., Kattes M. 1986; Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition, and description of Haloarcula gen.nov. and Haloferax gen. nov. Syst Appl Microbiol 8:89–99 [CrossRef]
    [Google Scholar]
  26. Ventosa A. 2001; Genus V. Haloferax . In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol 1 pp  315–318 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York, Berlin, Heidelberg: Springer;
  27. Vreeland R. H., Straight S., Krammes J., Dougherty K., Rosenzweig W. D., Kamekura M. 2002; Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. Extremophiles 6:445–452 [CrossRef]
    [Google Scholar]
  28. Wainø M., Tindall B. J., Ingvorsen K. 2000; Halorhabdus utahensis gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea from Great Salt Lake, Utah. Int J Syst Evol Microbiol 50:183–190 [CrossRef]
    [Google Scholar]
  29. Xin H., Itoh T., Zhou P., Suzuki K., Kamekura M., Nakase T. 2000; Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50:1297–1303 [CrossRef]
    [Google Scholar]
  30. Xue Y., Fan H., Ventosa A., Grant W. D., Jones B. E., Cowan D. A., Ma Y. 2005; Halalkalicoccus tibetensis gen. nov., sp. nov., representing a novel genus of haloalkaliphilic archaea. Int J Syst Evol Microbiol 55:2501–2505 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64674-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64674-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error