1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 6

sp. nov., a strongly haemolytic species Free

Abstract

A Gram-negative bacterium, strain MDA0585, isolated from a sputum culture, was characterized by a polyphasic approach. The 16S rRNA gene and a conserved portion of the DNA gyrase A gene were sequenced and analysed phylogenetically. Strain MDA0585 showed the closest relationships with ATCC 12472 and PRAA4-1 (96.1 % and 96.3 % 16S rRNA gene sequence similarity, respectively). The cellular fatty acids of strain MDA0585 consisted mainly of C, C 7 and C 6 (summed feature 3) and C 7 and C 6 (summed feature 8), a profile that was similar to, but distinguishable from, those of ATCC 12472 and PRAA4-1. In culture, strain MDA0585 differed from and in several ways: lack of violet pigmentation, the ability to haemolyse sheep blood, differences in several biochemical reactions and higher resistance to antibiotics. The culture supernatant of strain MDA0585 also caused remarkable haemolysis of human erythrocytes. These results suggest that strain MDA0585 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is MDA0585 (=CCUG 53230=JCM 14163=DSM 19808).

  • Published Online:
Keyword(s): RBC, red blood cells
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64681-0
2008-06-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/6/1398.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64681-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Thomas, L. M., Alejandro, A. S., Zhang, J., Zhang, Z., Webb, M. & Lipman, D. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  2. Brazilian National Genome Project Consortium(2003). The complete genome sequence of Chromobacterium violaceum reveals remarkable and exploitable bacterial adaptability. Proc Natl Acad Sci U S A 100, 11660–11665.[CrossRef] [Google Scholar]
  3. Clinical and Laboratory Standards Institute(2005).Performance Standards for Antimicrobial Susceptibility Testing; Fifteenth Informational Supplement, vol. 25, M100–S15. Wayne, PA: Clinical and Laboratory Standards Institute (formerly NCCLS).
  4. Dewhirst, F. E., Paster, B. J. & Bright, P. L.(1989).Chromobacterium, Eikenella, Kingella, Neisseria, Simonsiella, and Vitreoscilla species comprise a major branch of the beta group Proteobacteria by 16S ribosomal ribonucleic acid sequence comparison: transfer of Eikenella and Simonsiella to the family Neisseriaceae (emend). Int J Syst Bacteriol 39, 258–266.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1993).phylip (Phylogeny Inference Package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle.
  6. Han, X. Y. & Andrade, R. A.(2005).Brevundimonas diminuta infections and its resistance to fluoroquinolones. J Antimicrob Chemother 55, 853–859.[CrossRef] [Google Scholar]
  7. Han, X. Y., Pham, A. S., Nguyen, K. U., Smythe, W. R., Ordonez, N. G., Jacobson, K. L. & Tarrand, J. J.(2001). Pulmonary granuloma caused by Pseudomonas andersonii sp. nov. Am J Clin Pathol 116, 347–353.[CrossRef] [Google Scholar]
  8. Han, X. Y., Pham, A. S., Tarrand, J. J., Sood, P. K. & Luthra, R.(2002). Rapid and accurate identification of mycobacteria by sequencing hypervariable regions of the 16S ribosomal RNA gene. Am J Clin Pathol 118, 796–801.[CrossRef] [Google Scholar]
  9. Hungria, M., Astolfi-Filho, S., Chueire, L. M., Nicolas, M. F., Santos, E. B., Bulbol, M. R., Souza-Filho, A., Nogueira Assuncao, E., Germano, M. G. & Vasconcelos, A. T.(2005). Genetic characterization of Chromobacterium isolates from black water environments in the Brazilian Amazon. Lett Appl Microbiol 41, 17–23.[CrossRef] [Google Scholar]
  10. Jeong, Y., Kim, J., Kim, S., Kang, Y., Nagamatsu, T. & Hwang, I.(2003). Toxoflavin produced by Burkholderia glumae causing rice grain rot is responsible for inducing bacterial wilt in many field crops. Plant Dis 87, 890–895.[CrossRef] [Google Scholar]
  11. Kim, M. H., Lee, H. J., Suh, J. T., Chang, B. S. & Cho, K. S.(2005). A case of Chromobacterium infection after car accident in Korea. Yonsei Med J 46, 700–702.[CrossRef] [Google Scholar]
  12. Lee, J., Kim, J. S., Nahm, C. H., Choi, J. W., Kim, J., Pai, S. H., Moon, K. H., Lee, K. & Chong, Y.(1999). Two cases of Chromobacterium violaceum infection after injury in a subtropical region. J Clin Microbiol 37, 2068–2070. [Google Scholar]
  13. Logan, N. A.(1989). Numerical taxonomy of violet-pigmented, Gram-negative bacteria and description of Iodobacter fluviatile gen. nov., comb. nov. Int J Syst Bacteriol 39, 450–456.[CrossRef] [Google Scholar]
  14. Maeda, Y., Kiba, A., Ohnishi, K. & Hikichi, Y.(2004). Implications of amino acid substitutions in GyrA at position 83 in terms of oxolinic acid resistance in field isolates of Burkholderia glumae, a causal agent of bacterial seedling rot and grain rot of rice. Appl Environ Microbiol 70, 5613–5620.[CrossRef] [Google Scholar]
  15. Martin, P. A., Gundersen-Rindal, D., Blackburn, M. & Buyer, J.(2007).Chromobacterium subtsugae sp. nov., a betaproteobacterium toxic to Colorado potato beetle and other insect pests. Int J Syst Evol Microbiol 57, 993–999.[CrossRef] [Google Scholar]
  16. Rowe, G. E. & Welch, R. A.(1994). Assays of hemolytic toxins. Methods Enzymol 235, 657–667. [Google Scholar]
  17. Salanoubat, M., Genin, S., Artiguenave, F., Gouzy, J., Mangenot, S., Arlat, M., Billault, A., Brottier, P., Camus, J. C. & other authors(2002). Genome sequence of the plant pathogen Ralstonia solanacearum. Nature 415, 497–502.[CrossRef] [Google Scholar]
  18. Scholz, H. C., Witte, A., Tomaso, H., Al Dahouk, S. & Neubauer, H.(2005). Genotyping of Chromobacterium violaceum isolates by recA PCR-RFLP analysis. FEMS Microbiol Lett 244, 347–352.[CrossRef] [Google Scholar]
  19. Taipa, M. A., Liebeton, K., Costa, J. V., Cabral, J. M. & Jaeger, K. E.(1995). Lipase from Chromobacterium viscosum: biochemical characterization indicating homology to the lipase from Pseudomonas glumae. Biochim Biophys Acta 1256, 396–402.[CrossRef] [Google Scholar]
  20. Teoh, A. Y., Hui, M., Ngo, K. Y., Wong, J., Lee, K. F. & Lai, P. B.(2006). Fatal septicaemia from Chromobacterium violaceum: case reports and review of the literature. Hong Kong Med J 12, 228–231. [Google Scholar]
  21. Tettelin, H., Saunders, N. J., Heidelberg, J., Jeffries, A. C., Nelson, K. E., Eisen, J. A., Ketchum, K. A., Hood, D. W., Peden, J. F. & other authors(2000). Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science 287, 1809–1815.[CrossRef] [Google Scholar]
  22. Traub, P. C., Schmidt-Dannert, C., Schmitt, J. & Schmid, R. D.(2001). Gene synthesis, expression in E. coli, and in vitro refolding of Pseudomonas sp. KWI 56 and Chromobacterium viscosum lipases and their chaperones. Appl Microbiol Biotechnol 55, 198–204.[CrossRef] [Google Scholar]
  23. Voet, D. & Voet, J. G.(1995).Biochemistry. 2nd edn, p. 217. New York City: John Wiley & Sons.
  24. Wooley, P. G.(1905).Bacillus violaceous manilae (a pathogenic organism). Bull Johns Hopkins Hosp 16, 89 [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64681-0
Loading
Chromobacterium haemolyticum sp. nov., a strongly haemolytic species
Int J Syst Evol Microbiol 58, 1398 (2008); https://doi.org/10.1099/ijs.0.64681-0
/content/journal/ijsem/10.1099/ijs.0.64681-0
/content/journal/ijsem/10.1099/ijs.0.64681-0
Loading

Data & Media loading...

Supplements

Cellular fatty acid contents of strain MDA0585 and ATCC 12472 . [ PDF] 55 KB

PDF

Graphs showing the time course of the growth of strain MDA0585 and the lysis of human red blood cells by the culture supernatants. [ PDF] 20 KB

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error