1887

Abstract

A yellow-coloured, marine actinobacterium, designated SST-45, was isolated from sandy sediment under the surface of a beach and taxonomically characterized by physiological, chemotaxonomic and phylogenetic methods. The cells of the isolate were Gram-positive, aerobic, non-sporulating, non-motile, spherical cells that occurred singly, in pairs, in clusters or as short chains. The isolate grew at 10–37 °C, an initial pH 5.1–12.1 and in the presence of 5 % (w/v) NaCl. The organism possessed -2,6-diaminopimelic acid as the diagnostic diamino acid in the cell wall, MK-8(H) as the major menaquinone, a polar lipid profile including diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid, C and C as the major fatty acids, and a DNA G+C content of 72.4 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that the organism was related to members of the genera and . The closest neighbours were DSM 12652 (97.0 % sequence similarity) and KCTC 9134 (96.7 %). The combination of morphological and chemotaxonomic characters supported the assignment of the isolate to the genus . However, the organism is clearly distinguished phenotypically from the single described species of this genus, . Based on the data obtained, the organism has been assigned as a novel species, for which the name sp. nov. is proposed. The type strain is strain SST-45 (=JCM 13812=NRRL B-24464).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64696-0
2007-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/7/1391.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64696-0&mimeType=html&fmt=ahah

References

  1. Collins M. D., Dorsch M., Stackebrandt E. 1989; Transfer of Pimelobacter tumescens to Terrabacter gen. nov. as Terrabacter tumescens comb. nov. and of Pimelobacter jensenii to Nocardioides as Nocardioides jensenii comb. nov. Int J Syst Bacteriol 39:1–6 [CrossRef]
    [Google Scholar]
  2. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  3. Fitch W. M. 1971; Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  4. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. 1974; Nocardia coeliaca , Nocardia autotrophica , and the nocardin strain. Int J Syst Bacteriol 24:54–63 [CrossRef]
    [Google Scholar]
  5. Hopwood D. A., Bibb M. J., Chater K. F., Kieser T., Bruton C. J., Kieser H. M., Lydiate D. J., Smith C. P., Ward J. M., Schrempf H. 1985 Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  6. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  7. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp  173–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  8. Lee S. D. 2006; Kineococcus marinus sp. nov., isolated from marine sediment of the coast of Jeju, Korea. Int J Syst Evol Microbiol 56:1279–1283 [CrossRef]
    [Google Scholar]
  9. Lee S. D., Kang S.-O., Hah Y. C. 2000; Hongia gen. nov., a new genus of the order Actinomycetales . Int J Syst Evol Microbiol 50:191–199 [CrossRef]
    [Google Scholar]
  10. MacFaddin J. F. 1980 Biochemical Tests for Identification of Medical Bacteria , 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  11. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  12. Miller E. S., Woese C. R., Brenner S. 1991; Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov. Int J Syst Bacteriol 41:363–368 [CrossRef]
    [Google Scholar]
  13. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M. 1977; Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 27:104–117 [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., Hutchinson I. G., Caldicott A. B., Goodfellow M. 1980; Thin layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188:221–233 [CrossRef]
    [Google Scholar]
  15. Nesterenko O., Kvasnikov E. I., Nogina T. M. 1985; Nocardioidaceae fam. nov., a new family of the order Actinomycetales Buchanan 1917. Mikrobiol Zh 47:3–12
    [Google Scholar]
  16. Park Y. H., Yoon J. H., Shin Y. K., Suzuki K., Kudo T., Seino A., Kim H. J., Lee J. S., Lee S. T. 1999; Classification of ‘ Nocardioides fulvus ’ IFO 14399 and Nocardioides sp. ATCC 39419 in Kribbella gen. nov., as Kribbella flavida sp. nov. and Kribbella sandramycini sp. nov.. Int J Syst Evol Microbiol 49:743–752
    [Google Scholar]
  17. Prauser H. 1976; Nocardioides , a new genus of the order Actinomycetales . Int J Syst Bacteriol 26:58–65 [CrossRef]
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Schumann P., Prauser H., Rainey F. A., Stackebrandt E., Hirsch P. 1997; Friedmanniella antarctica gen. nov., sp. nov. an ll-diaminopimelic acid-containing actinomycete from Antarctic sandstone. Int J Syst Bacteriol 47:278–283 [CrossRef]
    [Google Scholar]
  20. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  21. Sohn K., Hong S. G., Bae K. S., Chun J. 2003; Transfer of Hongia koreensis Lee et al. 2000 to the genus Kribbella Park et al. 1999 as Kribbella koreensis comb. nov. Int J Syst Evol Microbiol 53:1005–1007 [CrossRef]
    [Google Scholar]
  22. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. 1997; Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47:479–491 [CrossRef]
    [Google Scholar]
  23. Staneck J. L., Roberts G. D. 1974; Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231
    [Google Scholar]
  24. Suzuki K., Komagata K. 1983; Pimelobacter gen. nov., a new genus of coryneform bacteria with ll-diaminopimelic acid in the cell wall. J Gen Appl Microbiol 29:59–71 [CrossRef]
    [Google Scholar]
  25. Tamura T., Yokota A. 1994; Transfer of Nocardioides fastidiosa Collins and Stackebrandt 1989 to the genus Aeromicrobium as Aeromicrobium fastidiosum comb. nov. Int J Syst Bacteriol 44:608–611 [CrossRef]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882
    [Google Scholar]
  27. Trujillo M. E., Kroppenstedt R. M., Schumann P., Martínez-Molina E. 2006; Kribbella lupini sp. nov., isolated from the roots of Lupinus angustifolius . Int J Syst Evol Microbiol 56:407–411 [CrossRef]
    [Google Scholar]
  28. Urzì C., Salamone P., Schumann P., Stackebrandt E. 2000 Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. Int J Syst Evol Microbiol 50529–536 [CrossRef]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64696-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64696-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error