1887

Abstract

Two novel species belonging to the genus are described on the basis of a polyphasic taxonomic approach. A total of 40 strains of Gram-negative, psychrotolerant, HS-producing bacteria were isolated from marine fish (cod and plaice) caught in the Baltic Sea off Denmark. Strains belonging to group 1 (seven strains) were a lactate-assimilating variant of with a G+C content of 44 mol%. The strains of group 2 (33 strains) utilized lactate, -acetylglucosamine and malate but did not produce DNase or ornithine decarboxylase. Their G+C content was 47 mol%. Phylogenetic analysis of the 16S rRNA gene sequence data placed the two novel species within the genus . Group 1 showed greatest sequence similarity with ATCC BAA-1205 (99.9 %). However, gene sequence analysis and DNA–DNA hybridization differentiated these isolates from , with 95.6 % sequence similarity and less than 57 % DNA relatedness, respectively. Group 2 strains shared more than 99 % 16S rRNA gene sequence similarity with the type strains of and , but sequence similarity (~85 %) and the results of DNA hybridization (~28 %) indicated that the new isolates represented a novel species. Furthermore, when compared to each other, the type strains of and had almost identical sequences and significantly high DNA reassociation values (76–83 %), indicating that they belonged to the same species. Based on the conclusions of this study, we propose the novel species sp. nov. (type strain T147=LMG 23744=NBRC 102030) for group 1 strains and sp. nov. (type strain S13=LMG 23746=NBRC 102032) for group 2 strains, and we propose that as a later heterotypic synonym of .

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2007-02-01
2024-03-28
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