1887

Abstract

An anaerobic, mesophilic, syntrophic fatty-acid-oxidizing bacterium, designated strain OL-4, was isolated as a co-culture with DSM 1535 from an anaerobic expanded granular sludge bed reactor used to treat an oleate-based effluent. Strain OL-4 degraded oleate, a mono-unsaturated fatty acid, and straight-chain fatty acids C–C in syntrophic association with DSM 1535. Even-numbered fatty acids were degraded to acetate and methane whereas odd-numbered fatty acids were degraded to acetate, propionate and methane. Branched-chain fatty acids were not degraded. The bacterium could not grow axenically with any other substrate tested and therefore is considered to be obligately syntrophic. Fumarate, sulfate, thiosulfate, sulfur and nitrate could not serve as electron acceptors for strain OL-4 to degrade oleate or butyrate. Cells of strain OL-4 were curved rods, formed spores and showed a variable response to Gram staining. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain OL-4 was most closely related to the fatty-acid-oxidizing, syntrophic bacterium sp. TB-6 (95 % similarity), subsp. DSM 2245 (94 % similarity) and DSM 16215 (93 % similarity). In addition to this moderate similarity, phenotypic and physiological characteristics, such as obligate syntrophy, spore formation and utilization of a broader substrate range, differentiated strain OL-4 from these species. Therefore strain OL-4 represents a novel species, for which the name sp. nov. is proposed. The type strain is OL-4 (=DSM 17840=JCM 13948).

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2007-03-01
2024-03-29
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