1887

Abstract

A rod-shaped, phototrophic, purple sulfur bacterium, strain JA124, was isolated in pure culture from a marine aquaculture pond, located near Bhimunipatnam, in a medium that contained 3 % NaCl (w/v). Strain JA124 is a Gram-negative, motile rod with a single polar flagellum. Strain JA124 has a requirement for NaCl, with optimum growth at 1.5–8.5 %, and tolerates up to 11 % NaCl. Intracellular photosynthetic membranes are of the vesicular type. Bacteriochlorophyll and probably carotenoids of the spirilloxanthin series are present as photosynthetic pigments. Strain JA124 was able to utilize sulfide, sulfate, thiosulfate, sulfite, thioglycollate and cysteine as sulfur sources. Strain JA124 was able to grow photolithoautotrophically, photolithoheterotrophically and photo-organoheterotrophically. Chemotrophic and fermentative growth could not be demonstrated. Strain JA124 lacks diazotrophic growth and acetylene reduction activity. Pyridoxal phosphate is required for growth. During growth on reduced sulfur sources as electron donors, sulfur is deposited intermediately as a number of small granules within the cell. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA124 clusters with species of the genus belonging to the class . The highest sequence similarities of strain JA124 were found with the type strains of (98 %), (95 %) and (93 %). However, DNA–DNA hybridization with DSM 15907 revealed relatedness of only 65 % with strain JA124. The DNA base composition of strain JA124 was 67 mol% G+C (by HPLC). Based on 16S rRNA gene sequence analysis, morphological and physiological characteristics and DNA–DNA hybridization studies, strain JA124 (=ATCC BAA-1316=JCM 13911) is sufficiently different from other species to merit its description as the type strain of a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64753-0
2007-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/6/1261.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64753-0&mimeType=html&fmt=ahah

References

  1. Arunasri K., Sasikala C., Ramana C. V., Süling J., Imhoff J. F. 2005; Marichromatium indicum sp. nov., a novel purple sulfur gammaproteobacterium from mangrove soil of Goa, India. Int J Syst Evol Microbiol 55:673–679 [CrossRef]
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  3. Caumette P., Imhoff J. F., Süling J., Matheron R. 1997; Chromatium glycolicum sp. nov., a moderately halophilic purple sulfur bacterium that uses glycolate as substrate. Arch Microbiol 167:11–18 [CrossRef]
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1989 phylip (phylogeny inference package), version 3.5.1. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  6. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  7. Imhoff J. F. 1988; Anoxygenic phototrophic bacteria. In Methods in Aquatic Bacteriology pp  207–240 Edited by Austin B. Chichester: Wiley;
    [Google Scholar]
  8. Imhoff J. F., Trüper H. G. 1980; Chromatium purpuratum , sp. nov., a new species of the Chromatiaceae . Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt 1 Orig [C] 1:61–69
    [Google Scholar]
  9. Imhoff J. F., Süling J., Petri R. 1998; Phylogenetic relationships among the Chromatiaceae , their taxonomic reclassification and description of the new generaAllochromatium , Halochromatium , Isochromatium , Marichromatium , Thiococcus , Thiohalocapsa and Thermochromatium . Int J Syst Bacteriol 48:1129–1143 [CrossRef]
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Pfennig N., Trüper H. G. 1989; Purple bacteria. In Bergey's Manual of Systematic Bacteriology vol  3 pp  1637–1653 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  14. Pfennig N., Trüper H. G. 1992; The family Chromatiaceae. In The Prokaryotes. A Handbook on the Biology of Bacteria, Ecophysiology, Isolation, Identification, Applications . , 2nd edn. pp  3200–3221 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. Berlin, Heidelberg, New York: Springer;
    [Google Scholar]
  15. Ramana Ch. V., Sasikala Ch., Arunasri K., Anil Kumar P., Srinivas T. N. R., Shivaji S., Gupta P., Suling J., Imhoff J. F. 2006; Rubrivivax benzoatilyticus sp. nov., an aromatic hydrocarbon-degrading purple betaproteobacterium. Int J Syst Evol Microbiol 56:2157–2164 [CrossRef]
    [Google Scholar]
  16. Sasikala K., Ramana Ch. V., Raghuveer Rao P., Subrahmanyam M. 1990; Photoproduction of hydrogen, nitrogenase and hydrogenase activities of free and immobilized whole cells of Rhodobacter sphaeroides OU 001. FEMS Microbiol Lett 72:23–28 [CrossRef]
    [Google Scholar]
  17. Sorokin D. Y., Tourova T. P., Antipov A. N., Muyzer G., Kuenen J. G. 2004; Anaerobic growth of the haloalkaliphilic denitrifying sulfur-oxidizing bacterium Thialkalivibrio thiocyanodenitrificans sp. nov. with thiocyanate. Microbiology 150:2435–2442 [CrossRef]
    [Google Scholar]
  18. Srinivas T. N. R., Anil Kumar P., Sasikala Ch., Ramana Ch. V., Süling J., Imhoff J. F. 2006; Rhodovulum marinum sp. nov., a novel phototrophic purple non-sulfur alphaproteobacterium from marine tides of Visakhapatnam, India. Int J Syst Evol Microbiol 56:1651–1656 [CrossRef]
    [Google Scholar]
  19. Strzeszewski B. 1913; Beiträge zur Kenntnis der Schwefelflora in der Umgebung von Krakau. Bull Acad Sci Cracovie [B]309–334 (in German)
    [Google Scholar]
  20. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  21. Trüper H. G. 1970; Culture and isolation phototrophic sulfur bacteria from the marine environment. Helgol Wiss Meeresunters 20:6–16 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64753-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64753-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Supplementary material 2

PDF

Supplementary material 3

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error