1887

Abstract

A yellow-pigmented, Gram-negative, motile, strictly aerobic, pleomorphic bacterium (strain TDMA-16) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). Strain TDMA-16 was slightly tolerant to gamma-ray irradiation and produced carotenoids, including zeaxanthin, nostoxanthin and an unknown carotenoid, effectively [1.7 mg (g dry cells)]. The DNA G+C content of strain TDMA-16 was 63.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed strain TDMA-16 in a distinct lineage in the family ; sequence data showed that strain TDMA-16 was most closely related to IFO 15500 (95.1 %), JSS7 (95.0 %), IFO 15498 (94.9 %), DSM 14444 (94.9 %) and IFO 15499 (94.5 %). The major fatty acids of strain TDMA-16 were C 6 (34.5 %) and C 7 (29.3 %). The presence of Q-10 as the main ubiquinone, the -specific sphingoglycolipid in the polar lipid profile and 2-hydroxy fatty acids, plus the absence of 3-hydroxy fatty acids, supported identification of this strain as a member of the genus . Phylogenetic distinctiveness and unique phenotypic characteristics differentiated strain TDMA-16 from closely related species. The results of polyphasic taxonomic analyses suggest that strain TDMA-16 represents a novel species, for which the name sp. nov. is proposed. The type strain is strain TDMA-16 (=NBRC 102120=DSM 18422=CCUG 53607).

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2007-07-01
2024-03-28
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