1887

Abstract

Two bacterial strains, designated BBH5 and BBH7, were isolated from a deep-sea sediment sample collected from the Chagos Trench of the Indian Ocean (1 ° 06′ S 7 ° 31′ E). Based on their 16S rRNA gene sequence similarity (99.9 %), level of DNA–DNA relatedness (93 %) and a number of similar phenotypic characteristics, the two strains are identified as representing the same species. Their phylogenetically nearest neighbours, based on 16S rRNA gene sequence similarity values (97.9–98.4 %), were identified as , , and . However, strains BBH5 and BBH7 could be distinguished from the above four species by a number of phenotypic characteristics, and levels of DNA–DNA relatedness between the two new isolates and these species were 35–42 %. Therefore, strains BBH5 and BBH7 are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BBH7 (=LMG 23457 =IAM 15353).

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2008-01-01
2024-03-28
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References

  1. Bhadra, B., Roy, P. & Chakraborty, R.(2005).Serratia ureilytica sp. nov., a novel urea-utilizing species. Int J Syst Evol Microbiol 55, 2155–2158.[CrossRef] [Google Scholar]
  2. Bhadra, B., Rao, S. R., Kumar, N. N., Chaturvedi, P., Sarkar, P. K. & Shivaji, S.(2007).Pichia cecembensis sp. nov., isolated from a papaya fruit (Carica papaya L., Caricaceae). FEMS Yeast Res 7, 579–584.[CrossRef] [Google Scholar]
  3. Breed, R. S.(1953). The Brevibacteriaceae fam. nov. of order Eubacteriales. Rias Commun VI Congr Int Microbiol Roma 1, 13–14. [Google Scholar]
  4. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  5. Collins, M. D., Jones, D., Keddie, R. M. & Sneath, P. H. A.(1980). Reclassification of Chromobacterium iodinum (Davis) in a redefined genus Brevibacterium (Breed) as Brevibacterium iodinum nom. rev., comb. nov. J Gen Microbiol 120, 1–10. [Google Scholar]
  6. Collins, M. D., Farrow, J. A. E., Goodfellow, M. & Minnikin, D. E.(1983).Brevibacterium casei sp. nov. and Brevibacterium epidermidis sp. nov. Syst Appl Microbiol 4, 388–395.[CrossRef] [Google Scholar]
  7. Gavrish, E. Y., Krauzova, V. I., Potekhina, N. V., Karasev, S. G., Plotnikova, E. G., Altyntseva, O. V., Korosteleva, L. A. & Evtushenko, L. I.(2004). Three new species of brevibacteria, Brevibacterium antiquum sp. nov., Brevibacterium aurantiacum sp. nov., and Brevibacterium permense sp. nov. Microbiology English translation of Mikrobiologiia 73, 176–183.[CrossRef] [Google Scholar]
  8. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze, B. & Augsten, K.(1997).Demetria terragena gen. nov., sp. nov., a new genus of actinobacteria isolated from compost soil. Int J Syst Bacteriol 47, 1129–1133.[CrossRef] [Google Scholar]
  9. Heyrman, J., Verbeeren, J., Schumann, P., Devos, J., Swings, J. & De Vos, P.(2004).Brevibacterium picturae sp nov., isolated from a damaged mural painting at the Saint-Catherine chapel (Castle Herberstein, Austria). Int J Syst Evol Microbiol 54, 1537–1541.[CrossRef] [Google Scholar]
  10. Holding, A. J. & Collee, J. G.(1971). Routine biochemical tests. Methods Microbiol 6A, 2–32. [Google Scholar]
  11. Ivanova, E. P., Christen, R., Alexeeva, Y. V., Zhukova, N. V., Gorshkova, N. M., Lysenko, A. M., Mikhailov, V. V. & Nicolau, D. V.(2004).Brevibacterium celere sp. nov., isolated from degraded thallus of a brown alga. Int J Syst Evol Microbiol 54, 2107–2111.[CrossRef] [Google Scholar]
  12. Jones, D. & Keddie, R. M.(1986). Genus Brevibacterium. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1301–1313. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  13. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  14. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  15. Laemmli, U. K.(1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef] [Google Scholar]
  16. Lee, S. D.(2006).Brevibacterium samyangense sp. nov., an actinomycete isolated from a beach sediment. Int J Syst Evol Microbiol 56, 1889–1892.[CrossRef] [Google Scholar]
  17. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  18. Minnikin, D. E., Alshamaony, L. & Goodfellow, M.(1975). Differentiation of Mycobacterium, Nocardia and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef] [Google Scholar]
  19. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M.(1980). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef] [Google Scholar]
  20. Pascual, C. & Collins, M. D.(1999).Brevibacterium avium sp. nov., isolated from poultry. Int J Syst Bacteriol 49, 1527–1530.[CrossRef] [Google Scholar]
  21. Raghukumar, C., Raghukumar, S., Sheelu, G., Gupta, S. M., Bagender, B. & Rao, B. R.(2004). Buried in time: culturable fungi in a deep-sea sediment core from the Chagos Trench, Indian Ocean. Deep Sea Res 51, 1759–1768.[CrossRef] [Google Scholar]
  22. Reddy, G. S. N., Aggarwal, R. K., Matsumoto, G. I. & Shivaji, S.(2000).Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50, 1553–1561.[CrossRef] [Google Scholar]
  23. Reddy, G. S. N., Prakash, J. S. S., Vairamani, M., Prabhakar, S., Matsumoto, G. I. & Shivaji, S.(2002).Planococcus antarcticus and Planococcus psychrophilus spp. nov., isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6, 253–262.[CrossRef] [Google Scholar]
  24. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  25. Sly, L. I., Blackall, L. L., Kraat, P. C., Tian-Shen, T. & Sangkhobol, V.(1986). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5, 139–156.[CrossRef] [Google Scholar]
  26. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  27. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  28. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  29. Wauters, G., Avesani, V., Laffineur, K., Charlier, J., Janssens, M., Bosterhaut, B. & Delmee, M.(2003).Brevibacterium lutescens sp. nov., from human and environmental samples. Int J Syst Evol Microbiol 53, 1321–1325.[CrossRef] [Google Scholar]
  30. Wauters, G., Haase, G., Avesani, V., Charlier, J., Janssens, M., Broeck, J. V. & Delmee, M.(2004). Identification of a novel Brevibacterium species isolate from humans and description of Brevibacterium sanguinis sp. nov. J Clin Microbiol 42, 2829–2832.[CrossRef] [Google Scholar]
  31. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
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[PDF file of Supplementary Tables S1 and S2](38 KB)

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Extended neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showing the phylogenetic relationship between sp. nov. strains BBH5 and BBH7 and all species of . [PDF file of Supplementary Fig. S1](18 KB)

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