1887

Abstract

A Gram-positive, spore-forming, rod-shaped halophilic bacterial strain, IGA7-4, was isolated from a mangrove area on Ishigaki Island (Japan), and was characterized taxonomically using a polyphasic approach. Strain IGA7-4 was strictly aerobic and non-motile and formed central endospores. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain IGA7-4 is affiliated to the genus , and exhibits sequence similarities of 99.6–98.0 % to the type strains of species. Levels of DNA–DNA relatedness between strain IGA7-4 and the type strains of species were 9.5–46.6 %. The DNA G+C content of strain IGA7-4 was 46.5 mol%. The cell-wall peptidoglycan type (Orn–Asp), major cellular fatty acids (anteiso-C, anteiso-C, iso-C and iso-C) and quinone type (MK-7) of the isolate support its affiliation to the genus . On the basis of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data, the isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IGA7-4 (=MBIC08268=IAM 15427=KCTC 13121).

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2007-11-01
2024-04-24
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References

  1. Amoozegar M. A., Malekzadeh F., Malik K. A., Schumann P., Spröer C. 2003; Halobacillus karajensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 53:1059–1063 [CrossRef]
    [Google Scholar]
  2. Claus D., Fahmy F., Rolf H. J., Tosunoglu N. 1983; Sporosarcina halophila sp. nov., an obligate, slightly halophilic bacterium from salt marsh soils. Syst Appl Bacteriol 4:496–506
    [Google Scholar]
  3. Collins M. D., Jones D. 1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometic deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Goto K., Omura T., Hara Y., Sadaie Y. 2000; Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus . J Gen Appl Microbiol 46:1–8 [CrossRef]
    [Google Scholar]
  7. Goto K., Mochida K. M., Asahara M., Suzuki M., Yokota A. 2002; Application of the hypervariable region of the 16S rDNA sequence as an index for the rapid identification of species in the genus Alicyclobacillus . J Gen Appl Microbiol 48:243–250 [CrossRef]
    [Google Scholar]
  8. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  9. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  10. Liu W. Y., Zeng J., Wang L., Dou Y. T., Yang S. S. 2005; Halobacillus dabanensis sp. nov. and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. Int J Syst Evol Microbiol 55:1991–1996 [CrossRef]
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  15. Spring S., Ludwig W., Marquez M. C., Ventosa A., Schleifer K.-H. 1996; Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp.nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46:492–496 [CrossRef]
    [Google Scholar]
  16. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  17. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  18. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53:687–693 [CrossRef]
    [Google Scholar]
  19. Yoon J. H., Kang K. H., Oh T. K., Park Y. H. 2004; Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 8:23–28 [CrossRef]
    [Google Scholar]
  20. Yoon J.-H., Kang S.-J., Lee C.-H., Oh H. W., Oh T.-K. 2005; Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 55:2413–2417 [CrossRef]
    [Google Scholar]
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