1887

Abstract

Two Gram-negative, non-spore-forming, motile and helical-shaped bacterial strains, K92 and K93, were isolated from sludge from a dye works in Korea, and their taxonomic positions were investigated by means of a polyphasic approach. Strains K92 and K93 grew optimally at 37 °C and pH 7.0–8.0 in the presence of 0.5 % (w/v) NaCl. They contained Q-10 as the predominant ubiquinone and C 7 as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified amino-group-containing lipids that were ninhydrin-positive. Their DNA G+C contents were 70.0 mol%. The 16S rRNA gene sequences of K92 and K93 showed no differences, and the two strains had a mean DNA–DNA relatedness of 93 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains K92 and K93 formed a distinct evolutionary lineage within the . The 16S rRNA gene sequences of strains K92 and K93 exhibited similarity values of less than 91.5 % with respect to the 16S rRNA gene sequences of other members of the . The two strains were distinguishable from phylogenetically related genera through differences in several phenotypic properties. On the basis of the phenotypic, phylogenetic and genetic data, strains K92 and K93 represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is K92 (=KCTC 12839=JCM 14346).

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2007-06-01
2024-03-28
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References

  1. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Guyoneaud R., Mouné S., Eatock C., Bothorel V., Hirschler-Réa A., Willison J., Duran R., Liesack W., Herbert R. other authors 2002; Characterization of three spiral-shaped purple nonsulfur bacteria isolated from coastal lagoon sediments, saline sulfur springs, and microbial mats: emended description of the genus Roseospira and description of Roseospira marina sp.nov., Roseospira navarrensis sp. nov., and Roseospira thiosulfatophila sp. nov.. Arch Microbiol 178:315–324 [CrossRef]
    [Google Scholar]
  4. Imhoff J. F., Petri R., Süling J. 1998 Reclassification of species of the spiral-shaped phototrophic purple non-sulfur bacteria of the α - Proteobacteria : description of the new genera Phaeospirillum gen.nov., Rhodovibrio gen. nov., Rhodothalassium gen. nov. and Roseospira gen. nov.as well as transfer of Rhodospirillum fulvum to Phaeospirillum fulvum comb. nov., of Rhodospirillum molischianum to Phaeospirillum molischianum comb. nov., of Rhodospirillum salinarum to Rhodovibrio salinarum comb. nov., of Rhodospirillum sodomense to Rhodovibrio sodomensis comb.nov., of Rhodospirillum salexigens to Rhodothalassium salexigens comb. nov. and of Rhodospirillum mediosalinum to Roseospira medisalina comb. nov. Int J Syst Bacteriol 48, 793–798 [CrossRef]
  5. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  6. Krieg N. R. 1984; Genus Aquaspirillum Hylemon, Wells, Krieg and Jannasch 1973. In Bergey's Manual of Systematic Bacteriology vol. 1 pp  72–90 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  7. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  8. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  9. Pfennig N., Lünsdorf H., Suling J., Imhoff J. F. 1997; Rhodospira trueperi gen. nov., spec. nov., a new phototrophic Proteobacterium of the alpha group. Arch Microbiol 16839–45 [CrossRef]
    [Google Scholar]
  10. Sakane T., Yokota A. 1994; Chemotaxonomic investigation of heterotrophic, aerobic and microaerophilic spirilla, the genera Aquaspirillum , Magnetospirillum and Oceanospirillum . Syst Appl Microbiol 17:128–134 [CrossRef]
    [Google Scholar]
  11. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids . Technical Note 101: Newark, DE: MIDI Inc;
    [Google Scholar]
  12. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  13. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  14. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  15. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  16. Yoon J.-H., Kang K. H., Park Y.-H. 2003; Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53:449–454 [CrossRef]
    [Google Scholar]
  17. Yurkov V., Stackebrandt E., Holmes A., Fuerst J. A., Hugenholtz P., Golecki J., Gad'on N., Gorlenko V. M., Kompantseva E. I., Drews G. 1994; Phylogenetic positions of novel aerobic, bacteriochlorophyll a -containing bacteria and description of Roseococcus thiosulfatophilus gen.nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 44:427–434 [CrossRef]
    [Google Scholar]
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