@article{mbs:/content/journal/ijsem/10.1099/ijs.0.64926-0, author = "An, Sun-Young and Ishikawa, Shu and Kasai, Hiroaki and Goto, Keiichi and Yokota, Akira", title = "Amphibacillus sediminis sp. nov., an endospore-forming bacterium isolated from lake sediment in Japan", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2007", volume = "57", number = "11", pages = "2489-2492", doi = "https://doi.org/10.1099/ijs.0.64926-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64926-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "BTB, bromothymol blue", abstract = "A facultatively anaerobic, moderately alkaliphilic, Gram-positive, spore-forming and rod-shaped bacterial strain, Shu-P-Ggiii25-2T, was isolated from lake sediment in Shizuoka, Japan, and was characterized taxonomically using a polyphasic approach. Analysis of the 16S rRNA gene sequence indicated that the novel isolate clustered with the type strain of Amphibacillus xylanus and it exhibited sequence similarities of 94.9–95.6 % to the type strains of species of the genus Amphibacillus. Isoprenoid quinones and oxidase and catalase activities were not detected for strain Shu-P-Ggiii25-2T. The DNA G+C content was 42.3 mol%, the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and the major cellular fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. On the basis of phylogenetic position and phenotypic properties, strain Shu-P-Ggiii25-2T represents a novel species of the genus Amphibacillus, for which the name Amphibacillus sediminis sp. nov. is proposed. The type strain is Shu-P-Ggiii25-2T (=MBIC08269T=IAM 15428T=KCTC 13120T). An emended description of the genus Amphibacillus is also given.", }