1887

Abstract

Three ionizing-radiation-resistant bacterial strains (designated KR-196, KR-198 and KR-200) were isolated from a sample of arid soil collected from a coastal desert in Chile. The soil sample was irradiated before serial dilution plating was performed using one-tenth-strength plate count agar. Phylogenetic analysis of the 16S rRNA gene sequences showed these organisms to represent a novel species of the genus , having sequence similarities of 87.3–90.8 % with respect to recognized species. Strains KR-196, KR-198 and KR-200 were aerobic and showed optimum growth at 30 °C and pH 6.5–8.0. The major respiratory menaquinone was MK-8. The predominant fatty acids in these strains were 16 : 17, 16 : 0, 15 : 16, 17 : 0 and 18 : 0. The DNA G+C content of strain KR-200 was 63.9 mol%. Strains KR-196, KR-198 and KR-200 were found to be resistant to >10 kGy gamma radiation. On the basis of the phylogenetic, chemotaxonomic and phenotypic data, strain KR-200 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KR-200 (=LMG 22246=CIP 109416).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64956-0
2007-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/7/1408.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64956-0&mimeType=html&fmt=ahah

References

  1. Albuquerque L., Simões C., Nobre M. F., Pino N. M., Battista J. R., Silva M. T., Rainey F. A., Da Costa M. S. 2005; Truepera radiovictrix gen. nov., sp. nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov.. FEMS Microbiol Lett 247161–169 [CrossRef]
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [CrossRef]
    [Google Scholar]
  3. de Groot A., Chapon V., Servant P., Christen R., Fischer-Le Saux M., Sommer S., Heulin T. 2005; Deinococcus deserti sp. nov., a gamma-radiation-tolerant bacterium from the Sahara Desert. Int J Syst Evol Microbiol 55:2441–2446 [CrossRef]
    [Google Scholar]
  4. Degryse E., Glansdorff N., Pierard A. 1978; A comparative analysis of extremely thermophilic bacteria belonging to the genus Thermus . Arch Microbiol 177:189–196
    [Google Scholar]
  5. Donato M. M., Seleiro E. A., da Costa M. S. 1990; Polar lipid and fatty acid composition of strains of the genus Thermus . Syst Appl Microbiol 13:234–239 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 1993 phylip (phylogeny inference package) version 3.5.1. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  7. Ferreira A. C., Nobre M. F., Rainey F. A., Silva M. T., Waite R., Burghardt J., Chung A. P., da Costa M. S. 1997; Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. Int J Syst Bacteriol 47:939–947 [CrossRef]
    [Google Scholar]
  8. Hirsch P., Gallikowski C. A., Siebert J., Peiss K., Kroppenstedt R., Schumann P., Stackebrandt E., Anderson R. 2004; Deinococcus frigens sp. nov., Deinococcus saxicola sp. nov., and Deinococcus marmoris sp. nov., low temperature and draught-tolerating, UV-resistant bacteria from continental Antarctica. Syst Appl Microbiol 27:636–645 [CrossRef]
    [Google Scholar]
  9. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  11. Kuykendall L. D., Roy M. A., O'Neill J. J., Devine T. E. 1988; Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . Int J Syst Bacteriol 38:358–361 [CrossRef]
    [Google Scholar]
  12. Lai W.-A., Kämpfer P., Arun A. B., Shen F.-T., Huber B., Rekha P. D., Young C.-C. 2006; Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L. Int J Syst Evol Microbiol 56:787–791 [CrossRef]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  14. Moreira C., Rainey F. A., Nobre M. F., da Silva M. T., da Costa M. S. 2000; Tepidimonas ignava gen. nov., sp. nov. a new chemolithoheterotrophic and slightly thermophilic member of the β - Proteobacteria . Int J Syst Evol Microbiol 50:735–742 [CrossRef]
    [Google Scholar]
  15. Prado A., da Costa M. S., Madeira V. M. C. 1988; Effect of growth temperature on the lipid composition of two strains of Thermus sp. J Gen Microbiol 134:1653–1660
    [Google Scholar]
  16. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. 1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092 [CrossRef]
    [Google Scholar]
  17. Rainey F. A., Ray K., Ferreira M., Gatz B. Z., Nobre N. F., Bagaley D., Rash B. A., Park M.-J., Earl A. M. other authors 2005; Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. Appl Environ Microbiol 71:5225–5235 [CrossRef]
    [Google Scholar]
  18. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Microbiology pp  409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Tindall B. J. 1989; Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum . FEMS Microbiol Lett 60:251–254 [CrossRef]
    [Google Scholar]
  20. Zhang Y. Q., Sun C. H., Li W. J., Yu L. Y., Zhou J. Q., Zhang Y. Q., Xu L. H., Jiang C. L. 2007; Deinococcus yunweiensis sp. nov., a gamma- and UV-radiation-resistant bacterium from China. Int J Syst Evol Microbiol 57:370–375 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64956-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64956-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error