1887

Abstract

A cellulose-decomposing bacterium, strain JB, was isolated from sediments along the Qijiang River, Zhongshan City, China. Results of morphological, biochemical and chemotaxonomic characterization and 16S rRNA gene sequence analysis revealed that strain JB belonged to the genus . Insertion sequence-PCR fingerprinting patterns, DNA base ratio analysis and DNA–DNA hybridization data showed that strain JB differed from recognized species of the genus . Based on polyphasic analysis, strain JB represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JB (=LMG 23926=CGMCC 1.6508=DSM 18832).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64968-0
2007-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2519.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64968-0&mimeType=html&fmt=ahah

References

  1. Buczolits S., Schumann P., Weidler G., Radax C., Busse H.-J. 2003; Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain. Int J Syst Evol Microbiol 53:1955–1960 [CrossRef]
    [Google Scholar]
  2. Collins M. D., Brown J., Jones D. 1988; Brachybacterium faecium gen. nov., sp. nov., a coryneform bacterium from poultry deep litter. Int J Syst Bacteriol 38:45–48 [CrossRef]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  4. Gal L., Pages S., Gaudin C., Belaich A., Reverbel-Leroy C., Tardif C., Belaich J. P. 1997; Characterization of the cellulolytic complex (cellulosome) produced by Clostridium cellulolyticum . Appl Environ Microbiol 63:903–909
    [Google Scholar]
  5. Grimont P. A. D. 1999; Taxonomy and classification of bacteria. In Manual of Clinical Microbiology , 7th edn. pp 249–259 Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  6. Gvozdyak O. R., Nogina T. M., Schumann P. 1992; Taxonomic study of the genus Brachybacterium : Brachybacterium nesterenkovii sp. nov. Int J Syst Bacteriol 42:74–78 [CrossRef]
    [Google Scholar]
  7. Halliwell G. 1974; Cellulose. In Methods of Enzymatic Analysis . pp 1132–1142 Edited by Bergmeyer H. U. New York: Academic Press;
  8. Hendrick C. W., Dolye J. D., Hugley B. 1995; A new solid medium for enumerating cellulose utilizing bacteria in soil. Appl Environ Microbiol 61:2016–2019
    [Google Scholar]
  9. Heyrman J., Balcaen A., De Vos P., Schumann P., Swings J. 2002; Brachybacterium fresconis sp. nov. and Brachybacterium sacelli sp. nov., isolated from deteriorated parts of a medieval wall painting of the chapel of Castle Herberstein (Austria). Int J Syst Evol Microbiol 52:1641–1646 [CrossRef]
    [Google Scholar]
  10. Hurek T., Egener T., Reinhold-Hurek B. 1997; Divergence in nitrogenases of Azoarcus spp., Proteobacteria of the β subclass. J Bacteriol 179:4172–4178
    [Google Scholar]
  11. Maidak B. L., Cole J. R., Parker C. T. Jr, Garrity G. M., Larsen N., Li B., Lilburn T. G., McCaughey M. J., Olsen G. J. other authors 1999; A new version of the RDP (Ribosomal Database Project. Nucleic Acids Res 27:171–173 [CrossRef]
    [Google Scholar]
  12. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  13. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology . pp 31–33 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  14. Peng G. X., Wang H. R., Zhang G. X., Hou W., Wang E. T., Tan Z. Y. 2006; Azospirillum melinis sp. nov., a group of diazotrophs isolated from tropical molasses grass. Int J Syst Evol Microbiol 56:1263–1271 [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Schubert K., Ludwig W., Springer N., Kroppenstedt R. M., Accolas J., Fiedler F. 1996; Two coryneform bacteria isolated from the surface of French gruyėre and Beaufort cheeses are new species of the genus Brachybacterium alimentarium sp.nov. and Brachybacterium tyrofermentans sp. nov.. Int J Syst Bacteriol 46:81–87 [CrossRef]
    [Google Scholar]
  18. Smibert R., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  19. Song Y.-J., Yang R.-F., Guo Z.-B., Zhang M.-L., Wang X.-H., Zhou F. 2000; Distinctness of spore and vegetative cellular fatty acid profiles of some aerobic endospore-forming bacilli. J Microbiol Methods 39:225–241 [CrossRef]
    [Google Scholar]
  20. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  21. Takeuchi M., Fang C. X., Yokota A. 1995; Taxonomic study of the genus Brachybacterium : proposal of Brachybacterium conglomeratum sp. nov., nom. rev., Brachybacterium paraconglomeratum sp. nov., and Brachybacterium rhamnosum sp. nov. Int J Syst Bacteriol 45:160–168 [CrossRef]
    [Google Scholar]
  22. Tan Z. Y., Kan F. L., Peng G. X., Wang E. T., Reinhold-Hurek B., Chen W. X. 2001; Rhizobium yanglingense sp. nov., isolated from arid and semi-arid regions in China. Int J Syst Evol Microbiol 51:909–914 [CrossRef]
    [Google Scholar]
  23. Van de Peer Y., De Wachter R. 1994; treecon for windows: a software package for the construction and drawing of evolutionary trees for the Microsoft windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  24. Veiga M., Esparis A., Fabregas J. 1983; Isolation of cellulolytic actinomycetes from marine sediments. Appl Environ Microbiol 46:286–287
    [Google Scholar]
  25. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler P., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  26. Xu M., Guo J., Cen Y., Zhong X., Cao W., Sun G. 2005; Shewanella decolorationis sp. nov., a dye-decolorizing bacterium isolated from activated sludge of a waste-water treatment plant. Int J Syst Evol Microbiol 55:363–368 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64968-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64968-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error