1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 3

sp. nov., a novel actinomycete isolated from a Philippine sponge () Free

Abstract

The taxonomic status of a marine actinomycete isolated from the sponge sp. collected from Cebu, Philippines, was established using both phenotypic and genotypic data. Strain SPE 10-1 exhibited chemotaxonomic and morphological characteristics that were consistent with those of members of the genus . It showed a strict requirement for salt and is the first obligate marine bacterium of the genus to be isolated. The principal isoprenoid quinone detected was MK-9(H). The fatty acid pattern consisted mainly of terminally branched iso and anteiso fatty acids. The DNA G+C content was 72.6 mol%. Analysis of the 16S rRNA gene sequence supported affiliation of the strain with the genus ; the type strain of was the closest phylogenetic relative (96 % sequence similarity). Sequence similarities of strain SPE 10-1 to other type strains of this genus were 93–95 %. It is proposed that strain SPE 10-1 should be classified in the genus as a representative of sp. nov. The type strain of is SPE 10-1 (=DSM 45019=CIP 109355).

  • Published Online:
Keyword(s): ASW, artificial seawater
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64971-0
2008-03-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/3/628.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64971-0&mimeType=html&fmt=ahah

References

  1. Embley, T. M., Wait, R., Dobson, G. & Goodfellow, M.(1987). Fatty acid composition in the classification of Saccharopolyspora hirsuta. FEMS Microbiol Lett 41, 131–135.[CrossRef] [Google Scholar]
  2. Goodfellow, M., Lacey, J., Athalye, M., Embley, T. M. & Bowen, T.(1989).Saccharopolyspora gregorii and Saccharopolyspora hordei: two new actinomycete species from fodder. J Gen Microbiol 135, 2125–2139. [Google Scholar]
  3. Gordon, R. E.(1967). The taxonomy of soil bacteria. In The Ecology of Soil Bacteria, pp. 293–321. Edited by T. R. G. Gray & D. Parkinson. Liverpool: Liverpool University Press.
  4. Hentschel, U., Schmid, M., Wagner, M., Fieseler, L., Gernert, C. & Hacker, J.(2001). Isolation and phylogenetic analysis of bacteria with antimicrobial activities from the Mediterranean sponges Aplysina aerophoba and Aplysina cavernicola. FEMS Microbiol Ecol 35, 305–312.[CrossRef] [Google Scholar]
  5. Korn-Wendisch, F., Kempf, A., Grund, E., Kroppenstedt, T. & Kutzner, H. J.(1989). Transfer of Faenia rectivirgula Kurup and Agre 1983 to the genus Saccharopolyspora Lacey and Goodfellow 1975, elevation of Saccharopolyspora hirsuta subsp. taberi Labeda 1987 to species level, and emended description of the genus Saccharopolyspora. Int J Syst Bacteriol 39, 430–441.[CrossRef] [Google Scholar]
  6. Kroppenstedt, R.(1985). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  7. Labeda, D. P.(1987). Transfer of the type strain of Streptomyces erthyraeus (Waksman 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov., and designation of a neotype strain for Streptomyces erythraeus. Int J Syst Bacteriol 37, 19–22.[CrossRef] [Google Scholar]
  8. Lacey, J. & Goodfellow, M.(1975). A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et sp. nov. J Gen Microbiol 88, 75–85.[CrossRef] [Google Scholar]
  9. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  10. Lu, Z., Liu, Z., Wang, L., Qi, W. & Goodfellow, M.(2001).Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp. nov., novel actinomycetes from soil. Int J Syst Evol Microbiol 51, 319–325. [Google Scholar]
  11. Lyman, J. & Fleming, R. H.(1940). Composition of sea water. J Mar Res 3, 134–146. [Google Scholar]
  12. MacFaddin, J. F.(1980).Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore, MD: Williams & Wilkins.
  13. Mertz, F. P & Yao, R. C.(1990).Saccharopolyspora spinosa sp. nov. isolated from soil collected in a sugar mill rum still. Int J Syst Bacteriol 40, 34–39.[CrossRef] [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  15. Mincer, T. J., Jensen, P. R., Kauffman, C. A. & Fenical, W.(2002). Widespread and persistent populations of a major new actinomycete taxon in ocean sediments. Appl Environ Microbiol 68, 5005–5011.[CrossRef] [Google Scholar]
  16. Minnikin, D. E., Alshamaony, L. & Goodfellow, M.(1975). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef] [Google Scholar]
  17. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  18. Oppenheimer, C. H. & ZoBell, C. E.(1952). The growth and viability of sixty-three species of marine bacteria as influenced by hydrostatic pressure. J Mar Res 11, 10–18. [Google Scholar]
  19. Pimentel-Elardo, S., Wehrl, M., Friedrich, A. B., Jensen, P. R. & Hentschel, U.(2003). Isolation of planctomycetes from Aplysina sponges. Aquat Microb Ecol 33, 239–245.[CrossRef] [Google Scholar]
  20. Scheuermayer, M., Gulder, T. A. M., Bringmann, G. & Hentschel, U.(2006).Rubritalea marina gen. nov., sp. nov., a marine representative of the phylum ‘Verrucomicrobia’, isolated from a sponge (Porifera). Int J Syst Evol Microbiol 56, 2119–2124.[CrossRef] [Google Scholar]
  21. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  22. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Environ Microbiol 28, 226–231. [Google Scholar]
  23. Strunk, O., Gross, O., Reichel, B. & other authors(2000).arb: a software environment for sequence data (http://www.mikro.biologie.tu-muenchen.de). Department of Microbiology, Technische Universität München, Germany.
  24. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  25. Zhou, Z. H., Liu, Z. H., Qian, Y. D., Kim, S. B. & Goodfellow, M.(1998).Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48, 53–58.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64971-0
Loading
Saccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge (Porifera)
Int J Syst Evol Microbiol 58, 628 (2008); https://doi.org/10.1099/ijs.0.64971-0
/content/journal/ijsem/10.1099/ijs.0.64971-0
/content/journal/ijsem/10.1099/ijs.0.64971-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error