RT Journal Article SR Electronic(1) A1 Yumoto, Isao A1 Hirota, Kikue A1 Nodasaka, Yoshinobu A1 Tokiwa, Yutaka A1 Nakajima, KenjiYR 2008 T1 Alkalibacterium indicireducens sp. nov., an obligate alkaliphile that reduces indigo dye JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 4 SP 901 OP 905 DO https://doi.org/10.1099/ijs.0.64995-0 PB Microbiology Society, SN 1466-5034, AB Indigo-reducing, obligately alkaliphilic strains A11T, F11 and F12 were isolated from indigo fermentation liquor obtained from Tokushima Prefecture, Shikoku, Japan. The isolates grew at pH 9.0–12.3, but not at pH 7.0–8.0. The optimum pH range for growth was 9.5–11.5. They were Gram-negative, facultatively anaerobic, rod-shaped strains with peritrichous flagella. The isolates grew in 0–14 % (w/v) NaCl, with optimum growth at 1–11 %. They grew at temperatures of 15–35 °C with optimum growth at around 20–30 °C. dl-Lactate was the major end product from d-glucose. No quinones were detected. The peptidoglycan type was A4α, l-Lys (l-Orn)–d-Asp. The major cellular fatty acids were C16 : 0, C16 : 17c and C18 : 19c. The DNA G+C contents were 47.0–47.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that the isolates belong to the genus Alkalibacterium. DNA–DNA hybridization revealed low relatedness values between the isolates and the three phylogenetically most closely related species, Alkalibacterium olivapovliticus, Alkalibacterium psychrotolerans and Alkalibacterium iburiense (<41 %). On the basis of phenotypic characteristics, including hydrolysis of cellulose and fermentation of carbohydrates, and chemotaxonomic characteristics, phylogenetic data and DNA–DNA relatedness data, it is concluded that the isolates merit classification as representatives of a novel species of the genus Alkalibacterium, for which the name Alkalibacterium indicireducens sp. nov. is proposed. The type strain of this species is A11T (=JCM 14232T=NCIMB 14253T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64995-0