RT Journal Article SR Electronic(1) A1 Kaksonen, Anna H. A1 Spring, Stefan A1 Schumann, Peter A1 Kroppenstedt, Reiner M. A1 Puhakka, Jaakko A.YR 2008 T1 Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 4 SP 833 OP 838 DO https://doi.org/10.1099/ijs.0.65025-0 PB Microbiology Society, SN 1466-5034, AB A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1T, was rod-shaped and motile. The temperature range for growth was 33–51 °C (optimum 44–46 °C) and the pH range was 6.0–7.5 (optimum pH 6.4–7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0. Strain RE35E1T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475T (92.0 % similarity). Strain RE35E1T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1T (=DSM 16058T=JCM 14019T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65025-0