1887

Abstract

The taxonomic position of an actinomycete isolated from radiation-polluted soil collected in Xinjiang Province, north-west China, was determined by using a polyphasic approach. The isolate, designated strain R97, had chemical and morphological properties characteristic of streptomycetes. An almost-complete 16S rRNA gene sequence of the isolate was generated and compared with corresponding sequences of representative streptomycetes. The 16S rRNA data not only supported the classification of the strain in the genus but also showed that it represented a distinct phyletic line that was most closely, albeit loosely, associated with three other thermotolerant organisms, namely NBRC 14748, NBRC 14749 and NBRC 14750. Strain R97 could be distinguished from these organisms based on a range of phenotypic properties. It is proposed that R97 (=CGMCC 4.3519 =DSM 41901) be classified as the type strain of a novel species in the genus , sp. nov. The organism was shown to be resistant to Co gamma radiation at a dose of 15 kGy.

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2007-11-01
2024-03-19
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References

  1. Albuquerque L., Simoes C., Nobre M. F., Pino N. M., Battista J. R., Silva M. T., Rainey F. A., da Costa M. S. 2005; Truepera radiovictrix gen. nov., sp. nov., a new radiation resistant species and the proposal of Trueperaceae fam. nov.. FEMS Microbiol Lett 247161–169 [CrossRef]
    [Google Scholar]
  2. Anderson A. W., Nordan H. C., Cain R. F., Parrish G., Duggan D. 1956; Studies on a radio-resistant Micrococcus . I. Isolation, morphology, cultural characteristics, and resistance to gamma radiation. Food Technol 10:575–577
    [Google Scholar]
  3. Brooks B. W., Murray R. G. E. 1981; Nomenclature for “ Micrococcus radioduran s” and other radiation-resistant cocci: Deinococcaceae fam. nov. and Deinococcus gen. nov., including five species. Int J Syst Bacteriol 31:353–360 [CrossRef]
    [Google Scholar]
  4. Chen M. Y., Wu S. H., Lin G. H., Lu C. P., Lin Y. T., Chang W. C., Tsay S. S. 2004; Rubrobacter taiwanensis sp. nov., a novel thermophilic, radiation-resistant species isolated from hot springs. Int J Syst Evol Microbiol 54:1849–1855 [CrossRef]
    [Google Scholar]
  5. Chun J., Goodfellow M. 1995; A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245 [CrossRef]
    [Google Scholar]
  6. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  7. Collins M. D., Hutson R. A., Grant I. R., Patterson M. F. 2000; Phylogenetic characterization of a novel radiation-resistant bacterium from irradiated pork: description of Hymenobacter actinosclerus sp. nov. Int J Syst Evol Microbiol 50:731–734 [CrossRef]
    [Google Scholar]
  8. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  9. Ferreira A. C., Nobre M. F., Rainey F. A., Silva M. T., Wait R., Burghardt J., Chung A. P., da Costa M. S. 1997; Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. Int J Syst Bacteriol 47:939–947 [CrossRef]
    [Google Scholar]
  10. Ferreira A. C., Nobre M. F., Moore E., Rainey F. A., Battista J. R., da Costa M. S. 1999; Characterization and radiation resistance of new isolates of Rubrobacter radiotolerans and Rubrobacter xylanophilus . Extremophiles 3:235–238 [CrossRef]
    [Google Scholar]
  11. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  12. Goodfellow M., Lacey J., Todd C. 1987; Numerical classification of thermophilic streptomycetes. J Gen Microbiol 133:3135–3149
    [Google Scholar]
  13. Hasegawa T., Takizawa M., Tanida S. 1983; A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322 [CrossRef]
    [Google Scholar]
  14. Jones K. L. 1949; Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. J Bacteriol 57:141–145
    [Google Scholar]
  15. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  16. Kämpfer P., Kroppenstedt R. M., Dott W. 1991; A numerical classification of the genera Streptomyces and Streptoverticillium using miniaturized physiological tests. J Gen Microbiol 137:1831–1891 [CrossRef]
    [Google Scholar]
  17. Kawato M., Shinobu R. 1959; On Streptomyces herbaricolor sp. nov., supplement: a single technique for microscopical observation. Mem Osaka Univ Lib Arts Educ B Nat Sci 8:114–119
    [Google Scholar]
  18. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  19. Kroppenstedt R. M. 1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp 179–199 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  20. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  21. Lechevalier H., Lechevalier M. P. 1980; The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy (Special Publication 6) pp 277–284 Edited by Dietz A., Thayer D. W. Arlington, VA: Society of Industrial Biology;
    [Google Scholar]
  22. Lechevalier M. P., De Bièvre C., Lechevalier H. A. 1977; Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5:249–260 [CrossRef]
    [Google Scholar]
  23. Manfio G. P., Zakrzewska-Czerwinska J., Atalan E., Goodfellow M. 1995; Towards minimal standards for the description of Streptomyces species. Biotechnologia 7:8242–263
    [Google Scholar]
  24. Marmur J., Doty P. 1962; Determination of base composition of deoxyribonucleic acid from its denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  25. Mattimore V., Battista J. R. 1996; Radioresistance of Deinococcus radiodurans : functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. J Bacteriol 178:633–637
    [Google Scholar]
  26. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  27. Phillips R. W., Wiegel J., Berry C. J., Fliermans C., Peacock A. D., White D. C., Shimkets L. J. 2002; Kineococcus radiotolerans sp. nov., a radiation-resistant, gram-positive bacterium. Int J Syst Evol Microbiol 52:933–938 [CrossRef]
    [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  29. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  30. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  31. Venkateswaran K., Kempf M., Chen F., Satomi M., Nicholson W., Kern R. 2003; Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant. Int J Syst Evol Microbiol 53:165–172 [CrossRef]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Bacterial Systematics. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  33. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. 1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
    [Google Scholar]
  34. Williams S. T., Goodfellow M., Alderson G. 1989; Genus Streptomyces Waksman and Henrici 1943, 339AL . In Bergey's Manual of Systematic Bacteriology vol. 4 pp 2452–2492 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  35. Xu C., Wang L., Cui Q., Huang Y., Liu Z., Zhang G., Goodfellow M. 2006; Neutrotolerant acidophilic Streptomyces species isolated from acidic soils in China: Streptomyces guanduensis sp. nov., Streptomyces paucisporeus sp. nov., Streptomyces rubidus sp.nov. and Streptomyces yanglinensis sp. nov. Int J Syst Evol Microbiol 56:1109–1115 [CrossRef]
    [Google Scholar]
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