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Volume 58, Issue 1

gen. nov., sp. nov., a freshwater bacterium belonging to the family Free

Abstract

A freshwater bacterium, designated IMCC1704, was isolated from a eutrophic pond. The strain was Gram-negative, oxidase-positive, catalase-negative, chemoheterotrophic and facultatively aerobic with cells that were motile rods with a single polar flagellum. Based on 16S rRNA gene sequence similarity analyses, the novel strain was most closely related to the genera (91.7 %), (89.0–91.2 %), (87.9–90.1 %), (87.2–89.5 %) and (87.8 %). Phylogenetic trees generated using 16S rRNA gene sequences showed that the novel isolate belonged to the family of the class and formed a distinct phyletic lineage within the family. The DNA G+C content of the strain was 63.1 mol% and the predominant constituents of the cellular fatty acids were C 7 and/or iso-C 2-OH (21.2 %), C 7 (12.8 %) and C 3-OH (12.3 %). These chemotaxonomic properties, together with several phenotypic characteristics, differentiated the novel strain from other members of the family . From the taxonomic data, which revealed the distant relationship of the new strain to the related genera, the strain should be classified as a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of sp. nov. is IMCC1704 (=KCCM 42363=NBRC 102354).

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2008-01-01
2024-04-20
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References

  1. Angelos, J. A., Spinks, P. Q., Ball, L. M. & George, L. W.(2007).Moraxella bovoculi sp. nov., isolated from calves with infectious bovine keratoconjunctivitis. Int J Syst Evol Microbiol 57, 789–795.[CrossRef] [Google Scholar]
  2. Bakermans, C., Ayala-del-Rio, H. L., Ponder, M. A., Vishnivetskaya, T., Gilichinsky, D., Thomashow, M. F. & Tiedje, J. M.(2006).Psychrobacter cryohalolentis sp. nov. and Psychrobacter arcticus sp. nov., isolated from Siberian permafrost. Int J Syst Evol Microbiol 56, 1285–1291.[CrossRef] [Google Scholar]
  3. Bogan, B. W., Sullivan, W. R., Kayser, K. J., Derr, K. D., Aldrich, H. C. & Paterek, J. R.(2003).Alkanindiges illinoisensis gen. nov., sp. nov., an obligately hydrocarbonoclastic, aerobic squalane-degrading bacterium isolated from oilfield soils. Int J Syst Evol Microbiol 53, 1389–1395.[CrossRef] [Google Scholar]
  4. Bouvet, P. J. M. & Grimont, P. A. D.(1986). Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacer junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwoffii. Int J Syst Bacteriol 36, 228–240.[CrossRef] [Google Scholar]
  5. Bozal, N., Montes, M. J., Tudela, E. & Guinea, J.(2003). Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp. nov. and Psychrobacter fozii sp. nov. Int J Syst Evol Microbiol 53, 1093–1100.[CrossRef] [Google Scholar]
  6. Brisou, J. & Prévot, A. R.(1954). Études de systématique bactérienne. X. Révision des espèces réunies dans le genre Achromobacter. Ann Inst Pasteur (Paris) 86, 722–728. [Google Scholar]
  7. Carr, E. L., Kämpfer, P., Patel, B. K., Gurtler, V. & Seviour, R. J.(2003). Seven novel species of Acinetobacter isolated from activated sludge. Int J Syst Evol Microbiol 53, 953–963.[CrossRef] [Google Scholar]
  8. Cho, J.-C. & Giovannoni, S. J.(2003).Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria. Int J Syst Evol Microbiol 53, 1031–1036.[CrossRef] [Google Scholar]
  9. Choo, Y. J., Lee, K., Song, J. & Cho, J. C.(2007).Puniceicoccus vermicola gen. nov., sp. nov., a novel marine bacterium, and description of Puniceicoccaceae fam. nov., Puniceicoccales ord. nov., Opitutaceae fam. nov., Opitutales ord. nov. and Opitutae classis nov. in the phylum ‘Verrucomicrobia’. Int J Syst Evol Microbiol 57, 532–537.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  11. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  12. Heuchert, A., Glockner, F. O., Amann, R. & Fischer, U.(2004).Psychrobacter nivimaris sp. nov., a heterotrophic bacterium attached to organic particles isolated from the South Atlantic (Antarctica). Syst Appl Microbiol 27, 399–406.[CrossRef] [Google Scholar]
  13. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York.
  14. Jung, S. Y., Lee, M. H., Oh, T. K., Park, Y. H. & Yoon, J. H.(2005).Psychrobacter cibarius sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 55, 577–582.[CrossRef] [Google Scholar]
  15. Juni, E. & Bøvre, K.(2005). Family II. Moraxellaceae Rossau, Van Landschoot, Gillis and De Ley 1991. 317VP. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol 2 (The Proteobacteria), part B (The Gammaproteobacteria), pp. 411–441. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  16. Juni, E. & Heym, G. A.(1986).Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of gram-negative, aerobic, oxidase-positive coccobacilli. Int J Syst Bacteriol 36, 388–391.[CrossRef] [Google Scholar]
  17. Kodjo, A., Tonjum, T., Richard, Y. & Bovre, K.(1995).Moraxella caprae sp. nov., a new member of the classical Moraxellae with very close affinity to Moraxella bovis. Int J Syst Bacteriol 45, 467–471.[CrossRef] [Google Scholar]
  18. Komagata, K. & Suzuki, K.(1987). Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  19. Kovacs, N.(1956). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703 [Google Scholar]
  20. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  21. Lwoff, A.(1939). Révision et démembrement des Hemophileae, le genre Moraxella nov. gen. Ann Inst Pasteur (Paris) 62, 168–176. [Google Scholar]
  22. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  23. Nemec, A., De Baere, T., Tjernberg, I., Vaneechoutte, M., van der Reijden, T. J. & Dijkshoorn, L.(2001).Acinetobacter ursingii sp. nov. and Acinetobacter schindleri sp. nov., isolated from human clinical specimens. Int J Syst Evol Microbiol 51, 1891–1899.[CrossRef] [Google Scholar]
  24. Nemec, A., Dijkshoorn, L., Cleenwerck, I., De Baere, T., Janssens, D., Van Der Reijden, T. J., Jezek, P. & Vaneechoutte, M.(2003).Acinetobacter parvus sp. nov., a small-colony-forming species isolated from human clinical specimens. Int J Syst Evol Microbiol 53, 1563–1567.[CrossRef] [Google Scholar]
  25. Nishmura, Y., Ino, T. & Ilzuka, H.(1988).Acinetobacter radioresistens sp. nov. isolated from cotton and soil. Int J Syst Bacteriol 38, 209–211.[CrossRef] [Google Scholar]
  26. Reasoner, D. J. & Geldreich, E. E.(1985). A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49, 1–7. [Google Scholar]
  27. Romanenko, L. A., Lysenko, A. M., Rohde, M., Mikhailov, V. V. & Stackebrandt, E.(2004).Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan. Int J Syst Evol Microbiol 54, 1741–1745.[CrossRef] [Google Scholar]
  28. Rossau, R., Van Landschoot, A., Gillis, M. & De Ley, J.(1991). Taxonomy of Moraxellaceae fam. nov., a new bacterial family to accommodate the genera Moraxella, Acinetobacter, and Psychrobacter and related organisms. Int J Syst Bacteriol 41, 310–319.[CrossRef] [Google Scholar]
  29. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  30. Simpson, J. M., Santo Domingo, J. W. & Reasoner, D. J.(2004). Assessment of equine fecal contamination: the search for alternative bacterial source-tracking targets. FEMS Microbiol Ecol 47, 65–75.[CrossRef] [Google Scholar]
  31. Song, J., Yang, S.-J. & Cho, J.-C.(2007). “Bring to lab” of 19 novel species among 60 isolates retrieved from a freshwater pond. J Microbiol Biotechnol 17, 168–175. [Google Scholar]
  32. Staley, J. T., Irgens, R. L. & Brenner, D. J.(1987).Enhydrobacter aerosaccus gen. nov., sp. nov., a gas-vacuolated, facultatively anaerobic, heterotrophic rod. Int J Syst Bacteriol 37, 289–291.[CrossRef] [Google Scholar]
  33. Swofford, D.(2002).paup*: Phylogenetic analysis using parsimony (*and other methods). Sunderland, MA: Sinauer Associates.
  34. Vandamme, P., Gillis, M., Vancanneyt, M., Hoste, B., Kersters, K. & Falsen, E.(1993).Moraxella lincolnii sp. nov., isolated from the human respiratory tract, and reevaluation of the taxonomic position of Moraxella osloensis. Int J Syst Bacteriol 43, 474–481.[CrossRef] [Google Scholar]
  35. Woo, P. C., Tse, H., Lau, S. K., Leung, K. W., Woo, G. K., Wong, M. K., Ho, C. M. & Yuen, K. Y.(2005).Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess. Syst Appl Microbiol 28, 316–322.[CrossRef] [Google Scholar]
  36. Xie, C. H. & Yokota, A.(2005). Transfer of the misnamed [Alysiella] sp. IAM 14971 (=ATCC 29468) to the genus Moraxella as Moraxella oblonga sp. nov. Int J Syst Evol Microbiol 55, 331–334.[CrossRef] [Google Scholar]
  37. Yoon, J. H., Lee, C. H., Kang, S. J. & Oh, T. K.(2005a).Psychrobacter celer sp. nov., isolated from sea water of the South Sea in Korea. Int J Syst Evol Microbiol 55, 1885–1890.[CrossRef] [Google Scholar]
  38. Yoon, J. H., Lee, C. H., Yeo, S. H. & Oh, T. K.(2005b).Psychrobacter aquimaris sp. nov. and Psychrobacter namhaensis sp. nov., isolated from sea water of the South Sea in Korea. Int J Syst Evol Microbiol 55, 1007–1013.[CrossRef] [Google Scholar]
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Perlucidibaca piscinae gen. nov., sp. nov., a freshwater bacterium belonging to the family Moraxellaceae
Int J Syst Evol Microbiol 58, 97 (2008); https://doi.org/10.1099/ijs.0.65039-0
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vol. , part 1, pp. 97 - 102

A transmission electron micrograph of a negatively stained cell of strain IMCC1704 . [ PDF] (111 KB)

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