1887

Abstract

A Gram-negative, rod-shaped, motile, non-spore-forming bacterium, designated strain A62-14B, was isolated from a constant-temperature, spring-fed, freshwater lake. On the basis of the complete 16S rRNA gene sequence, strain A62-14B was shown to belong to the class , being most closely related to sp. HTB082 (96.2 % sequence similarity), (95.01 %), (96.35 %), and (95.9 %). The major fatty acids (C 7, 38.56 %; C, 19.04 %; C 3-OH, 12.83 %; C 7, 7.70 %) and the motility of strain A62-14B support its affiliation to the genus . The salt intolerance of strain A62-14B, together with the results of other physiological and biochemical tests, allowed the differentiation of this strain from the three species of the genus with validly published names. Therefore strain A62-14B represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A62-14B (=ATCC BAA-1235=DSM 17496). The description of the genus is emended to reflect the halointolerance and freshwater origin of strain A62-14B.

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2007-10-01
2024-03-28
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References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Amy P. S., Haldeman D. L., Ringelberg D., Hall D. H., Russell C. 1992; Comparison of identification systems for classification of bacteria isolated from water and endolithic habitats within the deep subsurface. Appl Environ Microbiol 58:3367–3373
    [Google Scholar]
  3. Bochner B. R., Gadzinski P., Panomitros E. 2001; Phenotype microarrays for high-throughput phenotypic testing and assay of gene function. Genome Res 11:1246–1255 [CrossRef]
    [Google Scholar]
  4. Böckelmann U., Janke A., Kuhn R., Neu T. R., Wecke J., Lawrence J. R., Szewzyk U. 2006; Bacterial extracellular DNA forming a defined network-like structure. FEMS Microbiol Lett 262:31–38 [CrossRef]
    [Google Scholar]
  5. Brettar I., Christen R., Höfle M. G. 2002; Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. Int J Syst Evol Microbiol 52:1851–1857 [CrossRef]
    [Google Scholar]
  6. Brettar I., Christen R., Höfle M. G. 2006; Rheinheimera perlucida sp. nov., a marine bacterium of the Gammaproteobacteria isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 56:2177–2183 [CrossRef]
    [Google Scholar]
  7. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology . pp 21–33 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
  8. Fonnesbech Vogel B., Venkateswaran K., Christensen H., Falsen E., Christiansen G., Gram L. 2000 Polyphasic taxonomic approach in the description of Alishewanella fetalis gen. nov., sp. nov., isolated from a human foetus. Int J Syst Evol Microbiol 50, 1133–1142 [CrossRef]
  9. Halpern M., Senderovich Y., Snir S. 2007; Rheinheimera chironomi sp. nov., isolated from a chironomid (Diptera; Chironomidae) egg mass. Int J Syst Evol Microbiol 57:1872–1875 [CrossRef]
    [Google Scholar]
  10. Hillis D. M., Bull J. J. 1993; An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst Biol 42:182–192 [CrossRef]
    [Google Scholar]
  11. Huelsenbeck J. P., Ronquist F. 2001; mrbayes: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755 [CrossRef]
    [Google Scholar]
  12. Kämpfer P., Buczolits S., Albrecht A., Busse H.-J., Stackebrandt E. 2003; Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov. Int J Syst Evol Microbiol 53:893–896 [CrossRef]
    [Google Scholar]
  13. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. New York: Wiley;
    [Google Scholar]
  14. McLean R. J. C., Barnes M. B., Windham M. K., Merchant M. M., Forstner M. R. J., Fuqua C. 2005; Cell-cell influences on bacterial community development in aquatic biofilms. Appl Environ Microbiol 71:8987–8990 [CrossRef]
    [Google Scholar]
  15. Norton C. D., LeChevallier M. W. 2000; A pilot study of bacteriological population changes through potable water treatment and distribution. Appl Environ Microbiol 66:268–276 [CrossRef]
    [Google Scholar]
  16. Nylander J. A. A. 2004 MrModeltest 2.2. Distributed by the author Evolutionary Biology Centre, Uppsala University; Sweden: http://people.scs.fsu.edu/~nylander/mrmodeltest2/mrmodeltest2.html
    [Google Scholar]
  17. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
    [Google Scholar]
  18. Romanenko L. A., Uchino M., Falsen E., Zhukova N. V., Mikhailov V. V., Uchimura T. 2003; Rheinheimera pacifica sp. nov., a novel halotolerant bacterium isolated from deep sea water of the Pacific. Int J Syst Evol Microbiol 53:1973–1977 [CrossRef]
    [Google Scholar]
  19. Schmidt M., Prieme A., Stougaard P. 2007; Arsukibacterium ikkense gen. nov., sp. nov, a novel alkaliphilic, enzyme-producing γ -Proteobacterium isolated from a cold and alkaline environment in Greenland. Syst Appl Microbiol 30:197–201 [CrossRef]
    [Google Scholar]
  20. Swofford D. L. 2002 paup*: Phylogenetic analysis using parsimony (* and other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  22. Tolson D. L., Barrigar D. L., McLean R. J. C., Altman E. 1995; Expression of a non-agglutinating fimbria by Proteus mirabilis . Infect Immun 63:1127–1129
    [Google Scholar]
  23. Yoon J.-H., Park S. E., Kang S.-J., Oh T.-K. 2007; Rheinheimera aquimaris sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57:1386–1390 [CrossRef]
    [Google Scholar]
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