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Volume 58, Issue 4

gen. nov., sp. nov., a new member of the family Free

Abstract

Strain KV-677, a Gram-positive, aerobic, motile, rod-shaped bacterium, was isolated from park soil in Tokyo, Japan, and characterized. It grew well at 15–30 °C on nutrient agar and colonies were pale yellow. The cell-wall peptidoglycan contained diaminobutyric acid, glycine, alanine and glutamic acid and the muramic acid acyl type was acetyl. The predominant menaquinone was MK-12. Mycolic acids were not detected. The DNA G+C content was 70 mol%. 16S rRNA gene sequence analysis revealed that strain KV-677 fell within the cluster of the family and formed a separate lineage joining the genera , , and , showing 95.5–96.9 % sequence similarities with the type strains of the type species of the above genera. However, strain KV-677 clearly differed from these and other genera with relatively high sequence similarity in its chemotaxonomic characteristics. Therefore, it is proposed that strain KV-677 represents a novel species in a new genus, gen. nov., sp. nov., in the family . The type strain of is KV-677 (=NRRL B-24538 =NBRC 102123).

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Keyword(s): DAB, diaminobutyric acid , SEM, scanning electron microscopy and TEM, transmission electron microscopy
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2008-04-01
2024-04-20
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Microterricola viridarii gen. nov., sp. nov., a new member of the family Microbacteriaceae
Int J Syst Evol Microbiol 58, 1019 (2008); https://doi.org/10.1099/ijs.0.65070-0
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Scanning electron micrographs of cells of strain KV-677 cultured on nutrient agar for 8 h (left) and 10 days (right) at 27 °C. Bars, 1 µm.

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Scanning electron micrographs of cells of strain KV-677 cultured on nutrient agar for 8 h (left) and 10 days (right) at 27 °C. Bars, 1 µm.

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