1887

Abstract

Four closely related species, , , and , form a clade within the family ; the taxonomic status and phylogenetic position of this clade have remained ambiguous for many years. To resolve this ambiguity, we tested these species against other species of the for phylogenetic and phenotypic differences. Sequence identities for the 16S rRNA gene were ≥97.4 % among members of the group, but were ≤95.5 % for members of this group in comparison with type species of other genera of the (i.e. and , with which they overlap in G+C content, and , and , with which they do not overlap in G+C content). Combined analysis of the , , , and 16S rRNA gene sequences revealed that the species of the group form a tightly clustered clade, distinct from these other genera. Furthermore, phenotypic traits differentiated the group from other genera of the , and a panel of 13 biochemical tests discriminated members of the group from type strains of and . These results indicate that the four species of the group represent a lineage within the that is distinct from other genera. We therefore propose their reclassification in a new genus, gen. nov. is composed of four species: comb. nov. (the type species) (type strain ATCC 7744 =CAIM 329 =CCUG 13450 =CIP 103206 =DSM 507 =LMG 4414 =NCIMB 1281), comb. nov. (type strain ATCC 29985 =CCUG 20283 =CIP 104991 =NCIMB 2252), comb. nov. (type strain ATCC 43839 =CIP 103166 =LMG 14010 =NCIMB 2262) and comb. nov. (type strain ATCC BAA-104 =NCIMB 13582 =LMG 24053).

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2007-12-01
2024-03-28
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References

  1. Ast J. C., Dunlap P. V. 2005; Phylogenetic resolution and habitat specificity of members of the Photobacterium phosphoreum species group. Environ Microbiol 7:1641–1654 [CrossRef]
    [Google Scholar]
  2. Bang S. S., Baumann P., Nealson K. H. 1978; Phenotypic characterization of Photobacterium logei (sp. nov.), a species related to P. fischeri . Curr Microbiol 1:285–288 [CrossRef]
    [Google Scholar]
  3. Baumann L., Baumann P. 1977; Biology of the marine enterobacteria: genera Beneckea and Photobacterium . Annu Rev Microbiol 31:39–61 [CrossRef]
    [Google Scholar]
  4. Baumann P., Baumann L., Bang S. S., Woolkalis M. J. 1980; Reevaluation of the taxonomy of Vibrio , Beneckea , and Photobacterium : abolition of the genus Beneckea . Curr Microbiol 4:127–132 [CrossRef]
    [Google Scholar]
  5. Beijerinck M. W. 1889; Le Photobacterium luminosum , bactérie lumineuse de la Mer du Nord. Arch Neerl Sci Exactes Nat 23:401–405 (in French
    [Google Scholar]
  6. Bryant T. N. 1991; Software for the development and evaluation of probabilistic identification matrices. Comput Appl Biosci 7:189–193
    [Google Scholar]
  7. Bryant T. N. 2004; PIBWin – software for probabilistic identification. J Appl Microbiol 97:1326–1327 [CrossRef]
    [Google Scholar]
  8. Carson J., Higgins M. J., Wilson T. K., Gudkovs N., Bryant T. N. 2006; Aquatic Animal Health Subprogram. Vibrios of Aquatic Animals: Development of a National Standard Diagnostic Technology Final report . Project 01/628 Hobart: Fisheries Research & Development Corporation;
  9. Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A., Taourit S., Bocs S., Boursaux-Eude C., Chandler M. other authors 2003; The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens . Nat Biotechnol 21:1307–1313 [CrossRef]
    [Google Scholar]
  10. Egidius E., Wiik R., Andersen K., Hoff K. A., Hjeltnes B. 1986; Vibrio salmonicida sp. nov., a new fish pathogen. Int J Syst Bacteriol 36:518–520 [CrossRef]
    [Google Scholar]
  11. Farmer J. J. III, Janda J. M., Brenner F. W., Cameron D. N., Birkhead K. M., Brenner D. J., Krieg N. R., Staley J. T. 2005; Genus I. Vibrio Pacini 1854, 411AL . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 2 part B Edited by Garrity G. M. pp 494–546 New York: Springer;
    [Google Scholar]
  12. Farris J. S., Albert V. A., Källersjö M., Lipscomb D., Kluge A. G. 1996; Parsimony jackknifing outperforms neighbor-joining. Cladistics 12:99–124 [CrossRef]
    [Google Scholar]
  13. Goloboff P. A., Farris J. S., Nixon K. C. 2005; tnt: tree analysis using new technology. Version 1.0: http://www.zmuc.dk/public/phylogeny/TNT/Read_Me!.htm
    [Google Scholar]
  14. Graybeal A. 1998; Is it better to add taxa or characters to a difficult phylogenetic problem?. Syst Biol 47:9–17 [CrossRef]
    [Google Scholar]
  15. Harwood C. S., Bang S. S., Baumann P., Nealson K. H. 1980; Photobacterium logei sp. nov., nom. rev.; Beneckea nereida sp. nov., nom. rev.; and Beneckea gazogenes sp. nov., nom. rev. Int J Syst Bacteriol 30:655 [CrossRef]
    [Google Scholar]
  16. Hendrie M., Hodgkiss W., Shewan J. M. 1970; The identification, taxonomy and classification of luminous bacteria. J Gen Microbiol 64:151–169 [CrossRef]
    [Google Scholar]
  17. Hillis D. M. 1998; Taxonomic sampling, phylogenetic accuracy, and investigator bias. Syst Biol 47:3–8 [CrossRef]
    [Google Scholar]
  18. Huang C. Y., Garcia J. L., Patel B. K., Cayol J. L., Baresi L., Mah R. A. 2000; Salinivibrio costicola subsp. vallismortis subsp. nov., a halotolerant facultative anaerobe from Death Valley, and emended description of Salinivibrio costicola . Int J Syst Evol Microbiol 50:615–622 [CrossRef]
    [Google Scholar]
  19. Lunder T., Sørum H., Holstad G., Steigerwalt A. G., Mowinckel P., Brenner D. J. 2000; Phenotypic and genotypic characterization of Vibrio viscosus sp.nov. and Vibrio wodanis sp. nov. isolated from Atlantic salmon ( Salmo salar ) with ‘winter ulcer’. Int J Syst Evol Microbiol 50:427–450 [CrossRef]
    [Google Scholar]
  20. Reichelt J. L., Baumann P. 1973; Taxonomy of the marine, luminous bacteria. Arch Microbiol 94:283–330
    [Google Scholar]
  21. Ruby E. G., Nealson K. H. 1976; Symbiotic association of Photobacterium fischeri with the marine luminous fish Monocentris japonica , a model of symbiosis based on bacterial studies. Biol Bull 151:574–586 [CrossRef]
    [Google Scholar]
  22. Ruby E. G., Urbanowski M., Campbell J., Dunn A., Faini M., Gunsalus R., Lostroh P., Lupp C., McCann J. other authors 2005; Complete genome sequence of Vibrio fischeri : a symbiotic bacterium with pathogenic congeners. Proc Natl Acad Sci U S A 102:3004–3009 [CrossRef]
    [Google Scholar]
  23. Swofford D. L. 2003 paup*: Phylogenetic Analysis Using Parsimony (*and Other Methods), version 4.0B10 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  24. Thompson F. L., Hoste B., Thompson C. C., Goris J., Gomez-Gil B., Huys L., De Vos P., Swings J. 2002; Enterovibrio norvegicus gen. nov., sp. nov., isolated from the gut of turbot ( Scophthalmus maximus ) larvae: a new member of the family Vibrionaceae . Int J Syst Evol Microbiol 52:2015–2022 [CrossRef]
    [Google Scholar]
  25. Thompson F. L., Gevers D., Thompson C. C., Dawyndt P., Naser S., Hoste B., Munn C. B., Swings J. 2005; Phylogeny and molecular identification of vibrios on the basis of multilocus sequence analysis. Appl Environ Microbiol 71:5107–5115 [CrossRef]
    [Google Scholar]
  26. Thyssen A., Ollevier F. 2005; Genus II. Photobacterium Beijerinck 1889, 401AL. In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol. 2, part B pp 546–552 Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. New York: Springer;
  27. Varón A., Vinh L. S., Bomash I., Wheeler W. C. 2007 poy 4.0 Beta 1822 New York: American Museum of Natural History;
    [Google Scholar]
  28. Wheeler W. 1996; Optimization alignment: the end of multiple sequence alignment in phylogenetics?. Cladistics 12:1–9 [CrossRef]
    [Google Scholar]
  29. Willcox W. R., Lapage S. P., Bascomb S., Curtis M. A. 1973; Identification of bacteria by computer: theory and programming. J Gen Microbiol 77:317–330 [CrossRef]
    [Google Scholar]
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