1887

Abstract

The taxonomic positions of three strains of marine gliding bacteria, TISTR 1736, TISTR 1741 and TISTR 1750, isolated from the southern coastline of Thailand were evaluated by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the three isolates formed a distinct lineage within the family ‘’, phylum , and were related to the genus . The DNA G+C contents of the isolates were in the range 40–43 mol%. The major respiratory quinone was MK-7. The major cellular fatty acids were 16 : 15 (-5-hexadecenoic acid) and 15 : 0 (pentadecanoic acid). The major hydroxyl fatty acids were 3-OH 17 : 0 (3-hydroxyheptadecanoic acid), 3-OH 15 : 0 (3-hydroxypentadecanoic acid) and 3-OH 16 : 0 (3-hydroxyhexadecanoic acid). On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic data, these marine bacteria are considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is TISTR 1750 (=IAM 15448).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65087-0
2007-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/10/2275.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65087-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  5. Hall T. 2004; BioEdit. Biological sequence alignment editor for win95/98/NT/2K/XP http://www.mbio.ncsu.edu/BioEdit/bioedit.html
    [Google Scholar]
  6. Hosoya S., Yokota A. 2007; Flammeovirga kamogawensis sp. nov., isolated from coastal seawater in Japan. Int J Syst Evol Microbiol 57:1327–1330 [CrossRef]
    [Google Scholar]
  7. Hosoya S., Arunpairojana V., Suwannachart C., Kanjana-Opas A., Yokota A. 2006; Aureispira marina gen. nov., sp. nov., a gliding, arachidonic acid-containing bacterium isolated from the southern coastline of Thailand. Int J Syst Evol Microbiol 56:2931–2935 [CrossRef]
    [Google Scholar]
  8. Hudson J. A., Schofield K. M., Morgan H. W., Daniel R. M. 1989; Thermonema lapsum gen. nov., sp. nov., a thermophilic gliding bacterium. Int J Syst Bacteriol 39:485–487 [CrossRef]
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  11. Lewin R. A. 1970; Flexithrix dorotheae gen. et sp. nov. ( Flexibacterales ); and suggestions for reclassifying sheathed bacteria. Can J Microbiol 16:511–515 [CrossRef]
    [Google Scholar]
  12. Ludwig W., Klenk H.-P. 2001; Overview: a phylogenetic backbone and taxonomic framework for prokaryotic systematics. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp 49–65 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  14. Naganuma T., Horikoshi K. 1994; Cellular fatty acids of marine agarolytic gliding bacteria. Syst Appl Microbiol 17:125–127 [CrossRef]
    [Google Scholar]
  15. Nakagawa Y., Hamana K., Sakane T., Yamasato K. 1997 Reclassification of Cytophaga aprica (Lewin 1969) Reichenbach 1989 in Flammeovirga gen. nov. as Flammeovirga aprica comb.nov. and of Cytophaga diffluens ( ex Stanier 1940; emend. Lewin 1969) Reichenbach 1989 in Persicobacter gen. nov. as Persicobacter diffluens comb. nov. Int J Syst Bacteriol 47, 220–223 [CrossRef]
  16. Nakagawa Y., Sakane T., Suzuki M., Hatano K. 2002; Phylogenetic structure of the genera Flexibacter, Flexithrix and Microscilla deduced from 16S rRNA sequence analysis. J Gen Appl Microbiol 48:155–165 [CrossRef]
    [Google Scholar]
  17. Pinhassi J., Zweifel U. L., Hagström Å. 1997; Dominant marine bacterioplankton species found among colony-forming bacteria. Appl Environ Microbiol 63:3359–3366
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Saitou H., Miura K. I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629 [CrossRef]
    [Google Scholar]
  20. Shah H. N., Collins M. D. 1988; Proposal for reclassification of Bacteroides asaccharolyticus , Bacteroides gingivalis , and Bacteroides endodontalis in new genus, Porphyromonas . Int J Syst Bacteriol 38:128–131 [CrossRef]
    [Google Scholar]
  21. Sly L. I., Arunpairojana V. 1987; Isolation of manganese-oxidizing Pedomicrobium cultures from water by micromanipulation. J Microbiol Methods 6:177–182 [CrossRef]
    [Google Scholar]
  22. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Manual of Methods for General and Molecular Bacteriology . pp 607–654 Edited by Gerhardt P. Washington, DC: American Society for Microbiology;
  23. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga -like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. with Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp.nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652 [CrossRef]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  25. Uchino Y., Hirata A., Yokota A., Sugiyama J. 1998 Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen.nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov.,Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev. J Gen Appl Microbiol 44201–210 [CrossRef]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65087-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65087-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error