1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 2

sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay Free

Abstract

Two novel marine, Gram-negative, non-motile, catalase- and oxidase-positive, aerobic bacteria were isolated from coastal seawater in Tokyo Bay. Analysis of almost-complete 16S rRNA gene sequences showed that the two isolates are members of the genus , sharing highest 16S rRNA gene sequence similarity (96.5 %) with NBRC 16710. The DNA–DNA reassociation values between NBRC 16710 and these isolates were only 10–20 %, in contrast to the high DNA relatedness between the two isolates (89 %). At least 1 % (w/v) NaCl was required for growth. Cellular fatty acid profiles revealed C 7 as the major component and C 3-OH as the major hydroxy fatty acid. Ubiquinone-10 was detected as the major respiratory quinone. The G+C content of the genomic DNA of both strains was 69 mol%. On the basis of DNA–DNA hybridization data and physiological and chemotaxonomic characteristics, it is proposed that these strains should be placed in a novel species, sp. nov. The type strain is KKL-A5 (=NBRC 100637 =CIP 108500); KKL-B9 (=NBRC 100640) is a reference strain.

  • Published Online:
Keyword(s): DAPI, 4′,6-diamidino-2-phenylindole
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65103-0
2008-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/2/383.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65103-0&mimeType=html&fmt=ahah

References

  1. Adachi, J. & Hasegawa, M.(1996). molphy Version 2.3 – Programs for Molecular Phylogenetics Based on Maximum Likelihood. Computer Science Monograph no. 28. Tokyo: Institute of Statistical Mathematics.
  2. Berry, A., Janssens, D., Hümbelin, M., Jore, J. P., Hoste, B., Cleenwerck, I., Vancanneyt, M., Bretzel, W., Mayer, A. F. & other authors(2003).Paracoccus zeaxanthinifaciens sp. nov., a zeaxanthin-producing bacterium. Int J Syst Evol Microbiol 53, 231–238.[CrossRef] [Google Scholar]
  3. Brosius, J., Palmer, M. L., Kennedy, P. J. & Noller, H. F.(1978). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef] [Google Scholar]
  4. Buck, J. D.(1982). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  5. Button, D. K., Schut, F., Ouang, P., Martin, R. & Robertson, B. R.(1993). Viability and isolation of marine bacteria by dilution culture: theory procedures and initial results. Appl Environ Microbiol 59, 881–891. [Google Scholar]
  6. Connon, S. A. & Giovannoni, S. J.(2002). High-throughput methods for culturing microorganisms in very-low-nutrient media yield diverse new marine isolates. Appl Environ Microbiol 68, 3878–3885.[CrossRef] [Google Scholar]
  7. Cowan, S. T. & Steel, K. J.(1993).Manual for the Identification of Medical Bacteria, 3rd edn. Edited and revised by G. I. Barrow & R. K. A. Feltham. Cambridge: Cambridge University Press.
  8. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  9. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  10. Kelly, D. P., Rainey, F. A. & Wood, A. P.(2006). The genus Paracoccus. In The Prokaryotes. A Handbook on the Biology of Bacteria, 3rd edn, vol. 5, pp. 232–249. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  11. La, H.-J., Im, T.-W., Ten, L. N., Kang, S. M., Shin, D.-Y. & Lee, S.-T.(2005).Paracoccus koreensis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket (UASB) reactor. Int J Syst Evol Microbiol 55, 1657–1660.[CrossRef] [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  13. Minamisawa, K.(1990). Division of rhizobitoxine-producing and hydrogen-uptake positive strains of Bradyrhizobium japonicum by nifDKE sequence divergence. Plant Cell Physiol 31, 81–89. [Google Scholar]
  14. Nakagawa, Y. & Yamasato, K.(1993). Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139, 1155–1161.[CrossRef] [Google Scholar]
  15. Needleman, S. B. & Wunsch, C. D.(1970). A general method applicable to the search for similarities in the amino acid sequence of two proteins. J Mol Biol 48, 443–453.[CrossRef] [Google Scholar]
  16. Pukall, R., Laroche, M., Kroppenstedt, R. M., Schumann, P., Stackebrandt, E. & Ulber, R.(2003).Paracoccus seriniphilus sp. nov., an l-serine-dehydratase-producing coccus isolated from marine bryozoan Bugula plumosa. Int J Syst Evol Microbiol 53, 443–447.[CrossRef] [Google Scholar]
  17. Rüger, H.-J. & Krambeck, H.-J.(1994). Evaluation of the BIOLOG substrate metabolism system for classification of marine bacteria. Syst Appl Microbiol 17, 281–288.[CrossRef] [Google Scholar]
  18. Ruiz-Ponte, C., Cilia, V., Lambert, C. & Nicolas, J. L.(1998).Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus. Int J Syst Bacteriol 48, 537–542.[CrossRef] [Google Scholar]
  19. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  20. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc. http://www.midi-inc.com/media/pdfs/TechNote_101.pdf
  21. Takaichi, S., Maoka, T., Akimoto, N., Khan, S. T. & Harayama, S.(2006). Major carotenoid from Paracoccus schoinia NBRC 100637T is adonixanthin diglucoside. J Nat Prod 69, 1823–1825.[CrossRef] [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  23. Urakami, T., Araki, H., Oyanagi, H., Suzuki, K. & Komagata, K.(1990).Paracoccus aminophilus sp. nov. and Paracoccus aminovorans sp. nov., which utilize N,N-dimethylformamide. Int J Syst Bacteriol 40, 287–291.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65103-0
Loading
Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay
Int J Syst Evol Microbiol 58, 383 (2008); https://doi.org/10.1099/ijs.0.65103-0
/content/journal/ijsem/10.1099/ijs.0.65103-0
/content/journal/ijsem/10.1099/ijs.0.65103-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error