1887

Abstract

A Gram-negative bacterium, designated strain EMB34, was isolated from a wastewater treatment plant in Korea. Growth was observed between 10 and 40 °C (optimum, 25–35 °C) and between pH 6.0 and 9.5 (optimum, pH 7.5–8.0). The cells were non-motile rods, linked with extracellular fibrils. The predominant fatty acids of strain EMB34 were iso-C, C, iso-C G, iso-C 3-OH and iso-C 3-OH and the strain contained phosphatidylethanolamine and phosphatidylinositol as the polar lipids. The G+C content of the genomic DNA was 34.2 mol% and the major quinone was menaquinone-6. Comparative 16S rRNA gene sequence analyses showed that strain EMB34 formed a distinct phyletic line within the genus . The levels of 16S rRNA gene sequence similarity with other species were less than 94.5 %. On the basis of phenotypic, chemotaxonomic and molecular data, it is clear that strain EMB34 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is EMB34 (=KCTC 12610=DSM 17961).

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2007-09-01
2024-04-24
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References

  1. Aslam Z., Im W. T., Kim M. K., Lee S. T. 2005; Flavobacterium granuli sp. nov., isolated from granules used in a wastewater treatment plant. Int J Syst Evol Microbiol 55:747–751 [CrossRef]
    [Google Scholar]
  2. Bergey D. H., Harrison F. C., Breed R. S., Hammer B. W., Huntoon F. M. 1923 Bergey's Manual of Determinative Bacteriology , 1st edn. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  4. Bernardet J. F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  5. Cousin S., Pauker O., Stackebrandt E. 2007; Flavobacterium aquidurense sp. nov. and Flavobacterium hercynium sp. nov., from a hard-water creek. Int J Syst Evol Microbiol 57:243–249 [CrossRef]
    [Google Scholar]
  6. Felsenstein J. 2002 phylip (phylogeny inference package), version 3.6a. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  7. Gerhardt P., Murray R. G. M., Wood W. A., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654 Edited by Gerhardt P. Washington DC: American Society for Microbiology;
    [Google Scholar]
  8. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods Enzymol 1:138–146
    [Google Scholar]
  9. Humphry D. R., George A., Black G. W., Cummings S. P. 2001; Flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. Int J Syst Evol Microbiol 51:1235–1243
    [Google Scholar]
  10. Jeon C. O., Lim J. M., Lee J. M., Xu L. H., Jiang C. L., Kim C. J. 2005; Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55:1891–1896 [CrossRef]
    [Google Scholar]
  11. Kämpfer P., Young C. C., Sridhar K. R., Arun A. B., Lai W. A., Shen F. T., Rekha P. D. 2006; Transfer of [ Flexibacter ] sancti , [ Flexibacter ] filiformis , [ Flexibacter ] japonensis and [ Cytophaga ] arvensicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov. Int J Syst Evol Microbiol 56:2223–2228 [CrossRef]
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  13. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  14. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp 115–175 Edited by Stackebrandt E., Goodfellow M. Chichester, UK: John Wiley and Sons;
    [Google Scholar]
  15. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  16. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  17. McCammon S. A., Bowman J. P. 2000 Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp.nov., and Flavobacterium xanthum sp. nov., nom.rev. and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063 [CrossRef]
  18. Park M., Lu S., Ryu S. H., Chung B. S., Park W., Kim C. J., Jeon C. O. 2006; Flavobacterium croceum sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 56:2443–2447 [CrossRef]
    [Google Scholar]
  19. Park M., Ryu S. H., Vu T. H., Ro H. S., Yun P. Y., Jeon C. O. 2007; Flavobacterium defluvii sp. nov., isolated from activated sludge. Int J Syst Evol Microbiol 57:233–237 [CrossRef]
    [Google Scholar]
  20. Tamaki H., Hanada S., Kamagata Y., Nakamura K., Nomura N., Nakano K., Matsumura M. 2003; Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int J Syst Evol Microbiol 53:519–526 [CrossRef]
    [Google Scholar]
  21. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  22. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  23. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2004; Flavobacterium degerlachei sp. nov., Flavobacterium frigoris sp.nov. and Flavobacterium micromati sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54:85–92 [CrossRef]
    [Google Scholar]
  24. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2005; Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 55:769–772 [CrossRef]
    [Google Scholar]
  25. Wang Z.-W., Liu Y.-H., Dai X., Wang B.-J., Jiang C.-Y., Liu S.-J. 2006; Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 56:439–442 [CrossRef]
    [Google Scholar]
  26. Yoon J.-H., Kang S.-J., Oh T.-K. 2006; Flavobacterium soli sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:997–1000 [CrossRef]
    [Google Scholar]
  27. Zhang D. C., Wang H. X., Liu H. C., Dong X. Z., Zhou P. J. 2006; Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier. Int J Syst Evol Microbiol 56:2921–2925 [CrossRef]
    [Google Scholar]
  28. Zhu F., Wang S., Zhou P. 2003; Flavobacterium xinjiangense sp. nov. and Flavobacterium omnivorum sp. nov., novel psychrophiles from the China No. 1 glacier. Int J Syst Evol Microbiol 53:853–857 [CrossRef]
    [Google Scholar]
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