1887

Abstract

Three strains of budding, ellipsoid-shaped and rosette-forming bacteria were isolated from acidic -dominated boreal wetlands of northern Russia and were designated strains MPL7, MOB77 and SB2. The presence of crateriform pits and numerous fibrillar appendages on the cell surface and an unusual spur-like projection on one pole of the cell indicated a planctomycete morphotype. These isolates are moderately acidophilic, mesophilic organisms capable of growth at pH values between 4.2 and 7.5 (with an optimum at pH 5.0–6.2) and at temperatures between 4 and 32 °C (optimum 15–26 °C). The major fatty acids are C and C 7; the major quinone is MK-6. The G+C content of the DNA is 54.4–56.5 mol%. Strains MPL7, MOB77 and SB2 possess nearly identical 16S rRNA gene sequences and belong to the planctomycete lineage defined by the genus , being most closely related to DSM 3776 (86.9–87.1 % sequence similarity). However, strain MPL7 showed only 28 % DNA–DNA hybridization with DSM 3776. Compared with currently described members of the genus , the isolates from northern wetlands do not form long and distinctive stalks, have greater tolerance of acidic conditions and low temperatures, are more sensitive to NaCl, lack pigmentation and degrade a wider range of biopolymers. The data therefore suggest that strains MPL7, MOB77 and SB2 represent a novel genus and species, for which the name gen. nov., sp. nov., is proposed. Strain MPL7 (=ATCC BAA-1393 =VKM B-2452) is the type strain of .

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2007-11-01
2024-03-28
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References

  1. Boschker H. T. S., Nold S. C., Wellsbury P., Bos D., de Graaf W., Pel R., Parkes R. J., Cappenberg T. E. 1998; Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers. Nature 392:801–805 [CrossRef]
    [Google Scholar]
  2. Boschker H. T. S., de Graaf W., Koster M., Meyer-Reil L. A., Cappenberg T. E. 2001; Bacterial populations and processes involved in acetate and propionate consumption in anoxic brackish sediment. FEMS Microbiol Ecol 35:97–103 [CrossRef]
    [Google Scholar]
  3. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366
    [Google Scholar]
  4. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  5. Dedysh S. N., Pankratov T. A., Belova S. E., Kulichevskaya I. S., Liesack W. 2006; Phylogenetic analysis and in situ identification of bacteria community composition in an acidic Sphagnum peat bog. Appl Environ Microbiol 72:2110–2117 [CrossRef]
    [Google Scholar]
  6. Fuerst J. A. 1995; The planctomycetes: emerging models for microbial ecology, evolution and cell biology. Microbiology 141:1493–1506 [CrossRef]
    [Google Scholar]
  7. Fuerst J. A. 2004; Planctomycetes – a phylum of emerging interest for microbial evolution and ecology. WFCC Newsl 38:1–11 http://www.wfcc.info/NEWSLETTER/newsletter38/
    [Google Scholar]
  8. Fuerst J. A. 2005; Intracellular compartmentation in planctomycetes. Annu Rev Microbiol 59:299–328 [CrossRef]
    [Google Scholar]
  9. Fuerst J. A., Gwilliam H. G., Lindsay M., Lichanska A., Belcher C., Vickers J. E., Hugenholtz P. 1997; Isolation and molecular identification of planctomycete bacteria from postlarvae of the giant tiger prawn, Penaeus monodon . Appl Environ Microbiol 63:254–262
    [Google Scholar]
  10. Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. (editors) 1981 Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Giovannoni S. J., Schabtach E., Castenholz R. W. 1987; Isosphaera pallida , gen. nov., and comb. nov., a gliding, budding eubacterium from hot springs. Arch Microbiol 147:276–284 [CrossRef]
    [Google Scholar]
  12. Hirsch P., Müller M. 1985; Planctomyces limnophilus sp. nov., a stalked and budding bacterium from freshwater. Syst Appl Microbiol 6:276–280 [CrossRef]
    [Google Scholar]
  13. Ivanova A. O., Dedysh S. N. 2006; High abundance of Planctomycetes in anoxic layers of a Sphagnum peat bog. Microbiology (English translation of Mikrobiologiia 75:716–719
    [Google Scholar]
  14. Kerger B. D., Mancuso C. A., Nichols P. D., White D. C., Langworthy T., Sittig M., Schlesner H., Hirsch P. 1988; The budding bacteria, Pirellula and Planctomyces , with atypical 16S rRNA and absence of peptidoglycan, show eubacterial phospholipids and uniquely high proportions of long chain beta-hydroxy fatty acids in the lipopolysaccharide lipid A. Arch Microbiol 149:255–260 [CrossRef]
    [Google Scholar]
  15. König H., Schlesner H., Hirsch P. 1984; Cell wall studies on budding bacteria of the Planctomyces / Pasteuria group and on Prosthecomicrobium sp. Arch Microbiol 138:200–205 [CrossRef]
    [Google Scholar]
  16. Kulichevskaya I. S., Pankratov T. A., Dedysh S. N. 2006; Detection of representatives of the Planctomycetes in Sphagnum peat bogs by molecular and cultivation approaches. Microbiology (English translation of Mikrobiologiia 75:329–335
    [Google Scholar]
  17. Lindsay M. R., Webb R. I., Strous M., Jetten M. S., Butler M. K., Forde R. J., Fuerst J. A. 2001; Cell compartmentalisation in planctomycetes: novel types of structural organisation for the bacterial cell. Arch Microbiol 175:413–429 [CrossRef]
    [Google Scholar]
  18. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  19. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  20. Nichols P. D., Guckert J. B., White D. C. 1986; Determination of monounsaturated fatty acid double-bond position and geometry for microbial monocultures and complex consortia by capillary GC-MS of their dimethyl disulphide adducts. J Microbiol Methods 5:49–55 [CrossRef]
    [Google Scholar]
  21. Nichols D. S., Nichols P. D., McMeekin T. A. 1995; A new n-C31 : 9 polyene hydrocarbon from Antarctic bacteria. FEMS Microbiol Lett 125:281–285 [CrossRef]
    [Google Scholar]
  22. Owen R. J., Lapage S. P., Hill L. R. 1969; Determination of base composition from melting profiles in dilute buffers. Biopolymers 7:503–516 [CrossRef]
    [Google Scholar]
  23. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212 [CrossRef]
    [Google Scholar]
  24. Schlesner H. 1994; The development of media suitable for the microorganisms morphologically resembling Planctomyces spp., Pirellula spp., and other Planctomycetales from various aquatic habitats using dilute media. Syst Appl Microbiol 17:135–145 [CrossRef]
    [Google Scholar]
  25. Schlesner H., Stackebrandt E. 1986; Assignment of the genera Planctomyces and Pirella to a new family Planctomycetaceae fam. nov. and description of the order Planctomycetales ord. nov. Syst Appl Microbiol 8:174–176 [CrossRef]
    [Google Scholar]
  26. Sinninghe Damsté J. S., Rijpstra W. I. C., Schouten S., Fuerst J. A., Jetten M. S. M., Strous M. 2004; The occurrence of hopanoids in planctomycetes: implications for the sedimentary biomarker record. Org Geochem 35:561–566 [CrossRef]
    [Google Scholar]
  27. Sittig M., Schlesner H. 1993; Chemotaxonomic investigation of various prosthecate and/or budding bacteria. Syst Appl Microbiol 16:92–103 [CrossRef]
    [Google Scholar]
  28. Staley J. T., Fuerst J. A., Giovannoni S., Schlesner H. 1992; The order Planctomycetales and the genera Planctomyces , Pirellula , Gemmata and Isosphaera . In The Prokaryotes: a Handbook on the Biology of Bacteria. Ecophysiology, Isolation, Identification, Applications , 2nd edn. pp 3710–3731 Edited by Balows A., Trüper H., Dworkin M., Harder W., Schleifer K. H. New York: Springer;
    [Google Scholar]
  29. Ward N., Rainey F. A., Stackebrandt E., Schlesner H. 1995; Unraveling the extent of diversity within the order Planctomycetales . Appl Environ Microbiol 61:2270–2275
    [Google Scholar]
  30. Ward N., Staley J. T., Fuerst J. A., Giovannoni S., Schlesner H., Stackebrandt E. 2006; The order Planctomycetales , including the genera Planctomyces, Pirellula, Gemmata and Isosphaera and the Candidatus genera Brocadia, Kuenenia and Scalindua. In The Prokaryotes: a Handbook on the Biology of Bacteria , 3rd edn. vol 7 pp 757–793 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. New York: Springer;
    [Google Scholar]
  31. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
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