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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 9

gen. nov., sp. nov., a nephridial symbiont of the earthworm (Savigny) Free

Abstract

A Gram-negative, flagellated, heterotrophic, catalase-negative, rod-shaped bacterium previously identified as an earthworm symbiont was isolated from nephridia of the earthworm . Comparisons of 16S rRNA gene sequences indicated its relatedness to the betaproteobacterial genus and the novel isolates shared 92–94 % sequence similarity with recognized species of this genus. Gene sequence phylogenies revealed that the group of earthworm symbionts formed a cohesive and independent clade. The DNA G+C content was 67.0±0.2 mol%. Major fatty acids were C, C 7 and C cyclo. While capable of growing in fully aerated media, all isolates favoured low oxygen concentrations and all required biotin or a mix of amino acids in order to grow on defined mineral media. Based on phylogenies inferred from three housekeeping gene sequences (, and ), DNA–DNA hybridization values, the unique ecology and the distinct physiology of the novel strains, the new genus gen. nov. is proposed for the earthworm nephridial symbionts. The name sp. nov. is proposed for the type species with strain EF01-2 (=ATCC BAA-1489=DSM 19286) as the type strain of the type species.

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Keyword(s): ACM, Acidovorax complex medium , ACMP, ACM Phytagel; CFA, cyclic fatty acids , CIA, chloroform-isoamyl alcohol , FAME, fatty acid methyl esters , FISH, fluorescence in situ hybridization , ML, maximum-likelihood , MLST, multilocus sequence typing , MP, maximum-parsimony , NJ, neighbour-joining , PCIA, phenol/chloroform-isoamyl alcohol , PFGE, pulsed field gel electrophoresis , PHB, polyhydroxybutyrate and TEM, transmission electron microscopy
© SGM

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gen. nov., sp. nov., a nephridial symbiont of the earthworm (Savigny)
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2008-09-01
2024-04-25
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Verminephrobacter eiseniae gen. nov., sp. nov., a nephridial symbiont of the earthworm Eisenia foetida (Savigny)
Int J Syst Evol Microbiol 58, 2147 (2008); https://doi.org/10.1099/ijs.0.65174-0
/content/journal/ijsem/10.1099/ijs.0.65174-0
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Vitamin requirements for growth on defined medium. [ PDF] 153 KB

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Siderophore production assayed through the CAS method. [ PDF] 54 KB

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Details of soil DNA surveys and siderophore production and uptake. [ PDF] 77 KB

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