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Volume 57, Issue 11

gen. nov., sp. nov., a novel thermoacidophilic, cell wall-less archaeon in the order , isolated from a solfataric soil in Hakone, Japan Free

Abstract

A novel thermoacidophilic, cell wall-less archaeon, strain IC-189, was isolated from a solfataric field in Ohwaku-dani, Hakone, Japan. The cells were irregular cocci, sometimes lobed, club-shaped or catenated, and were highly variable in size, ranging from 0.8 to 8.0 μm in diameter. The strain grew at temperatures in the range 38–68 °C (optimally at 60 °C) and at pH 1.8–4.0 (optimally at around pH 3.0). Strain IC-189 was obligately aerobic and heterotrophic, requiring yeast extract for growth. Yeast extract, glucose and mannose served as carbon and energy sources. The polar lipids consisted mainly of cyclic or acyclic glycerol-bisdiphytanyl-glycerol tetraethers, and the predominant quinone was a menaquinone with seven isoprenoid units (MK-7). The G+C content of total DNA was 56.1 mol%. 16S rRNA gene sequence analysis revealed that strain IC-189 was a member of the order , but diverged from the hitherto known species of the genera , and (86.2–91.0 % sequence similarity). These phenotypic and phylogenetic properties clearly support a separate taxonomic status for this strain. Therefore, strain IC-189 represents a novel genus (order ) and species, for which the name gen. nov., sp. nov. is proposed, with type strain IC-189 (=JCM 13583=DSM 18835).

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2007-11-01
2024-04-23
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Thermogymnomonas acidicola gen. nov., sp. nov., a novel thermoacidophilic, cell wall-less archaeon in the order Thermoplasmatales, isolated from a solfataric soil in Hakone, Japan
Int J Syst Evol Microbiol 57, 2557 (2007); https://doi.org/10.1099/ijs.0.65203-0
/content/journal/ijsem/10.1099/ijs.0.65203-0
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