1887

Abstract

Two marine, heterotrophic, aerobic, pigmented and gliding bacteria, isolated from the sea urchin were subjected to a polyphasic taxonomy study. The 16S rRNA gene sequence analysis revealed that strains KMM 6032 and KMM 6047 formed a distinct branch within the genus , a member of the family The level of sequence similarity between the novel isolates and members of the genus was 94.5–98.9 %. The DNA G+C content was 39–40 mol%. The dominant fatty acids were iso-C, iso-C, C, C 6, iso-C 3-OH, iso-C 9, iso-C 3-OH and summed feature 3 (comprising iso-C 2-OH and/or C 7). The results of DNA–DNA hybridization experiments supported by phenotypic data indicated that the isolates represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is KMM 6032 (=KCTC 22013=LMG 22574).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65251-0
2007-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/11/2655.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65251-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  2. Cho S.-H., Han J.-H., Seong C. N., Kim S. B. 2006; Phylogenetic diversity of acidophilic sporoactinobacteria isolated from various soils. J Microbiol 44:600–606
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  5. Ivanova E. P., Nedashkovskaya O. I., Chun J., Lysenko A. M., Frolova G. M., Svetashev V. I., Vysotskii M. V., Mikhailov V. V., Huq A., Colwell R. R. 2001; Arenibacter gen. nov., new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov.. Int J Syst Evol Microbiol 51:1987–1995 [CrossRef]
    [Google Scholar]
  6. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  7. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  9. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  10. Nedashkovskaya O. I., Suzuki M., Vysotskii M. V., Mikhailov V. V. 2003; Arenibacter troitsensis sp. nov., isolated from marine bottom sediment. Int J Syst Evol Microbiol 53:1287–1290 [CrossRef]
    [Google Scholar]
  11. Nedashkovskaya O. I., Kim S. B., Han S. K., Lysenko A. M., Mikhailov V. V., Bae K. S. 2004; Arenibacter certesii sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata . Int J Syst Evol Microbiol 54:1173–1176 [CrossRef]
    [Google Scholar]
  12. Nedashkovskaya O. I., Vancanneyt M., Cleenwerck I., Snauwaert C., Kim S. B., Lysenko A. M., Shevchenko L. S., Lee K. H., Park M. S. other authors 2006; Arenibacter palladensis sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata, and emended description of the genus Arenibacter . Int J Syst Evol Microbiol 56:155–160 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65251-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65251-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error