1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 2

sp. nov., isolated from soil from a racecourse in South Africa Free

Abstract

A novel actinomycete, designated strain S1.4, was isolated from a soil sample collected from Kenilworth Racecourse in the Western Cape, South Africa. The strain was able to grow in the presence of 5 % NaCl. It contained -diaminopimelic acid and glycine in the cell-wall peptidoglycan with glucose present in the whole-cell sugar profile. Strain S1.4 was shown to be a member of either the genus or the genus based on a rapid molecular identification method by using single-enzyme restriction endonuclease digestion of the PCR-amplified 16S rRNA gene with I, I, I, BI, I and I. Analysis of the 16S rRNA gene sequence indicated that strain S1.4 belonged to the genus . Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that strain S1.4 was related most closely to DSA1. Strain S1.4 was phenotypically distinct from DSA1 and was shown to be a separate genomic species based on DNA–DNA hybridization experiments (40.4±3.8 % DNA–DNA relatedness between the two). Strain S1.4 (=DSM 19227 =NRRL B-24553) is thus presented as the type strain of a novel species, for which the name sp. nov. is proposed.

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65278-0
2008-02-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/2/443.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65278-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  2. Atlas, R. M.(2004).Handbook of Microbiological Media, 3rd edn. Boca Raton, FL: CRC Press.
  3. Carlsohn, M. R., Groth, I., Spröer, C., Schütze, B., Saluz, H.-P., Munder, T. & Stackebrandt, E.(2007).Kribbella aluminosa sp. nov., isolated from a medieval alum slate mine. Int J Syst Evol Microbiol 57, 1943–1947.[CrossRef] [Google Scholar]
  4. Cook, A. E. & Meyers, P. R.(2003). Rapid identification of filamentous actinomycetes to the genus level using genus-specific 16S rRNA gene restriction fragment patterns. Int J Syst Evol Microbiol 53, 1907–1915.[CrossRef] [Google Scholar]
  5. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  6. Euzéby, J. P.(2007).Kribbella Park et al. 1999 , gen. nov. In List of Prokaryotic Names with Standing in Nomenclature. http://www.bacterio.cict.fr/ijk/kribbella.html
  7. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping of aerobic Actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  8. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  9. Kirby, B. M., Le Roes, M. & Meyers, P. R.(2006).Kribbella karoonensis sp. nov. and Kribbella swartbergensis sp. nov., isolated from soil from the Western Cape, South Africa. Int J Syst Evol Microbiol 56, 1097–1101.[CrossRef] [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  11. Lechevalier, M. P. & Lechevalier, H.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  12. Nonomura, H. & Ohara, Y.(1971). Distribution of actinomycetes in soil. VIII. Green spore group of Microtetraspora, its preferential isolation and taxonomic characteristics. J Ferment Technol 49, 1–7. [Google Scholar]
  13. Park, Y. H., Yoon, J. H., Shin, Y. K., Suzuki, K., Kudo, T., Seino, A., Kim, H., Lee, J. S. & Lee, S. T.(1999). Classification of ‘Nocardioides fulvus’ IFO 14399 and Nocardioides sp. ATCC 39419 in Kribbella gen. nov., as Kribbella flavida sp. nov. and Kribbella sandramycini sp. nov. Int J Syst Bacteriol 49, 743–752.[CrossRef] [Google Scholar]
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989). Bacterial media, antibiotics and bacterial strains. In Molecular Cloning: a Laboratory Manual, 2nd edn, pp. A.1–A.13. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  16. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  17. Sohn, K., Hong, S. G., Bae, K. S. & Chun, J.(2003). Transfer of Hongia koreensis Lee et al. 2000 to the genus Kribbella Park et al. 1999 as Kribbella koreensis comb. nov. Int J Syst Evol Microbiol 53, 1005–1007.[CrossRef] [Google Scholar]
  18. Song, J., Kim, B. Y., Hong, S. B., Cho, H. S., Sohn, K., Chun, J. & Suh, J. W.(2004).Kribbella solani sp. nov. and Kribbella jejuensis sp. nov., isolated from potato tuber and soil in Jeju, Korea. Int J Syst Evol Microbiol 54, 1345–1348.[CrossRef] [Google Scholar]
  19. Takahashi, K. & Nei, M.(2000). Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution, and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17, 1251–1258.[CrossRef] [Google Scholar]
  20. Tan, G. Y. A., Ward, A. C. & Goodfellow, M.(2006). Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media. Syst Appl Microbiol 29, 557–569.[CrossRef] [Google Scholar]
  21. Wang, Y., Zhang, Z. & Ruan, J.(1996). A proposal to transfer Microbispora bispora (Lechevalier 1965) to a new genus, Thermobispora gen. nov., as Thermobispora bispora comb. nov. Int J Syst Bacteriol 46, 933–938.[CrossRef] [Google Scholar]
  22. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  23. Williams, S. T., Goodfellow, M. & Alderson, G.(1989). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65278-0
Loading
Kribbella hippodromi sp. nov., isolated from soil from a racecourse in South Africa
Int J Syst Evol Microbiol 58, 443 (2008); https://doi.org/10.1099/ijs.0.65278-0
/content/journal/ijsem/10.1099/ijs.0.65278-0
/content/journal/ijsem/10.1099/ijs.0.65278-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error