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Volume 58, Issue 1

sp. nov., a non-pigmented, extremely halophilic archaeon from a deep-sea, hypersaline anoxic basin of the Red Sea, and emended description of the genus Free

Abstract

An extremely halophilic archaeon was isolated from a sample of the brine–sediment interface of the Shaban Deep in the northern Red Sea. Phylogenetic analysis of the 16S rRNA gene sequence revealed a close proximity to (99.3 %), the sole species of the genus . Strain SARL4B formed non-pigmented colonies and showed optimum growth at 45 °C, in 27 % (w/v) NaCl and at pH 6.5–7.0. This organism utilized a few complex substrates, such as yeast extract and starch, for growth. Strain SARL4B grew under anaerobic and microaerophilic conditions but grew extremely poorly under aerobic conditions. The ether lipids were diphytanyl derivatives. The DNA G+C content of the type strain was 61.7 mol%. On the basis of the phylogenetic data and physiological and biochemical characteristics, strain SARL4B represents a novel species of the genus , for which the name is proposed. The type strain is SARL4B (=DSM 18392=JCM 14471). An emended description of the genus is also proposed.

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2008-01-01
2024-04-19
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References

  1. Antón, J., Llobet-Brossa, E., Rodríguez-Valera, F. & Amann, R.(1999). Fluorescence in situ hybridization analysis of the prokaryotic community inhabiting crystallizer ponds. Environ Microbiol 1, 517–523.[CrossRef] [Google Scholar]
  2. Antunes, A.(2003). Microbial life at the anaerobic brine/seawater and sediment/brine interfaces. In Black Sea-Mediterranean-Red Sea, Cruise No. 52, January 2–March 27, 2002, pp. 50–52. Edited by J. Pätzold, G. Bohrmann & C. Hübscher. Hamburg: Universität Hamburg.
  3. Antunes, A., Eder, W., Fareleira, P., Santos, H. & Huber, R.(2003).Salinisphaera shabanensis gen. nov., sp. nov., a novel, moderately halophilic bacterium from the brine-seawater interface of the Shaban Deep, Red Sea. Extremophiles 7, 29–34. [Google Scholar]
  4. Antunes, A., França, L., Rainey, F. A., Huber, R., Nobre, M. F., Edwards, K. J. & da Costa, M. S.(2007).Marinobacter salsuginis sp. nov., isolated from the brine-seawater interface of the Shaban Deep, Red Sea. Int J Syst Evol Microbiol 57, 1035–1040.[CrossRef] [Google Scholar]
  5. Balch, W. E., Fox, G. E., Magrum, L. J., Woese, C. R. & Wolfe, R. S.(1979). Methanogens: reevaluation of a unique biological group. Microbiol Rev 43, 260–296. [Google Scholar]
  6. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M.(1977). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef] [Google Scholar]
  7. DasSarma, S. & Arora, P.(2001). Halophiles. In Encyclopedia of Life Sciences. Chichester: John Wiley. http://www.els.net
  8. Degens, E. & Ross, D. A.(1969). Hot brines and recent heavy metal deposits in the Red Sea. New York: Springer.
  9. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  10. Dussault, H. P.(1955). An improved technique for staining red halophilic bacteria. J Bacteriol 70, 484–485. [Google Scholar]
  11. Dyall-Smith, M.(2001).The Halohandbook: Protocols for Halobacterial Genetics, edn 4.5. Melbourne: University of Melbourne.
  12. Eder, W., Ludwig, W. & Huber, R.(1999). Novel 16S rRNA gene sequences retrieved from highly saline brine sediments of Kebrit Deep, Red Sea. Arch Microbiol 172, 213–218.[CrossRef] [Google Scholar]
  13. Eder, W., Jahnke, L. L., Schmidt, M. & Huber, R.(2001). Microbial diversity of the brine-seawater interface of the Kebrit Deep, Red Sea, studied via 16S rRNA gene sequences and cultivation methods. Appl Environ Microbiol 67, 3077–3085.[CrossRef] [Google Scholar]
  14. Eder, W., Schmidt, M., Koch, M., Garbe-Schönberg, D. & Huber, R.(2002). Prokaryotic phylogenetic diversity and corresponding geochemical data of the brine-seawater interface of the Shaban Deep, Red Sea. Environ Microbiol 4, 758–763.[CrossRef] [Google Scholar]
  15. Fiala, G., Woese, C. R., Langworthy, T. A. & Stetter, K. O.(1990).Flexistipes sinusarabici, a novel genus and species of eubacteria occurring in the Atlantis II Deep brines of the Red Sea. Arch Microbiol 154, 120–126.[CrossRef] [Google Scholar]
  16. Huber, R., Burggraf, S., Mayer, T., Barns, S. M., Rossnagel, P. & Stetter, K. O.(1995). Isolation of a hyperthermophilic archaeum predicted by in situ RNA analysis. Nature 376, 57–58.[CrossRef] [Google Scholar]
  17. Huber, R., Huber, H. & Stetter, K. O.(2000). Towards the ecology of hyperthermophiles: biotopes, new isolation strategies and novel metabolic properties. FEMS Microbiol Rev 24, 615–623.[CrossRef] [Google Scholar]
  18. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  19. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  21. Michaelis, W., Jenisch, A. & Richnow, H. H.(1990). Hydrothermal petroleum generation in Red Sea sediments from the Kebrit and Shaban Deeps. Appl Geochem 5, 103–114.[CrossRef] [Google Scholar]
  22. Moissl, C., Rudolph, C., Rachel, R., Koch, M. & Huber, R.(2003).In situ growth of the novel SM1 euryarchaeon from a string-of-pearls-like microbial community in its cold biotope, its physical separation and insights into its structure and physiology. Arch Microbiol 180, 211–217.[CrossRef] [Google Scholar]
  23. Nielsen, P., Fritze, D. & Priest, F. G.(1995). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141, 1745–1761.[CrossRef] [Google Scholar]
  24. Oren, A., Ventosa, A. & Grant, W. D.(1997). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef] [Google Scholar]
  25. Ross, H. N. M. & Grant, W. D.(1985). Lipids in archaebacterial taxonomy. In Chemical Methods in Bacterial Systematics, pp. 289–300. Edited by M. Goodfellow & D. E. Minnikin. Orlando, FL: Academic Press.
  26. Scientific Staff of Cruise Bannock 1984–12(1985). Gypsum precipitation from cold brines in an anoxic basin in the eastern Mediterranean. Nature 314, 152–154.[CrossRef] [Google Scholar]
  27. Smibert, R. M. & Krieg, N. R.(1981). General characterization. In Manual of Methods for General Bacteriology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  28. Tindall, B. J.(1989). Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum. FEMS Microbiol Lett 60, 251–254.[CrossRef] [Google Scholar]
  29. Trüper, H. G.(1969). Bacterial sulfate reduction in the Red Sea hot brines. In Hot Brines and Recent Heavy Metal Deposits in the Red Sea, pp. 263–271. Edited by E. T. Degens & D. A. Ross. New York: Springer.
  30. van der Wielen, P. W., Bolhuis, H., Borin, S., Daffonchio, D., Corselli, C., Giuliano, L., D'Auria, G., de Lange, G. J., Huebner, A. & other authors(2005). The enigma of prokaryotic life in deep hypersaline anoxic basins. Science 307, 121–123.[CrossRef] [Google Scholar]
  31. Vreeland, R. H., Straight, S., Krammes, J., Dougherty, K., Rosenzweig, W. D. & Kamekura, M.(2002).Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. Extremophiles 6, 445–452.[CrossRef] [Google Scholar]
  32. Wainø, M., Tindall, B. J. & Ingvorsen, K.(2000).Halorhabdus utahensis gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea from the Great Salt Lake, Utah. Int J Syst Evol Microbiol 50, 183–190.[CrossRef] [Google Scholar]
  33. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  34. Wiesenburg, D. A., Brooks, J. M. & Bernard, B. B.(1985). Biogenic hydrocarbon gases and sulfate reduction in the Orca basin brine. Geochim Cosmochim Acta 49, 2069–2080.[CrossRef] [Google Scholar]
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Halorhabdus tiamatea sp. nov., a non-pigmented, extremely halophilic archaeon from a deep-sea, hypersaline anoxic basin of the Red Sea, and emended description of the genus Halorhabdus
Int J Syst Evol Microbiol 58, 215 (2008); https://doi.org/10.1099/ijs.0.65316-0
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vol. , part 1, pp. 215 - 220

Polar lipid profiles of and strain SARL4B separated by mono-dimensional TLC. [ PDF] (106 KB)

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