@article{mbs:/content/journal/ijsem/10.1099/ijs.0.65335-0, author = "Nam, Ji-Hyun and Bae, Wookeun and Lee, Dong-Hun", title = "Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "5", pages = "1109-1113", doi = "https://doi.org/10.1099/ijs.0.65335-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65335-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-positive, rod-shaped, spore-forming bacterium, strain S-11T, was isolated from the activated sludge of a Bacillus-dominated wastewater treatment system in South Korea and was characterized using a polyphasic approach in order to determine its taxonomic position. Cells (0.5–0.6×2.0–2.2 μm) were motile by means of a single subpolar flagellum. They bore ellipsoidal endospores that lay in a central position in swollen sporangia. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S-11T was a member of the genus Oceanobacillus. 16S rRNA gene sequence similarity values and DNA–DNA relatedness of strain S-11T to the type strains of other Oceanobacillus species were less than 96.2 and 66.0 %, respectively. Strain S-11T showed distinct differences in the G+C content of the genomic DNA (33.6 mol%). The major cellular fatty acids were iso-C14 : 0, iso-C15 : 0, anteiso-C15 : 0 and iso-C16 : 0. The major isoprenoid quinone was MK-7. There were also some physiological differences in comparison with the type strains of Oceanobacillus species: tests for production of acetoin and acid production from dulcitol, erythritol, myo-inositol and sorbitol were positive. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain S-11T from the six Oceanobacillus species and subspecies with validly published names. Strain S-11T therefore represents a novel species, for which the name Oceanobacillus caeni sp. nov. is proposed, with the type strain S-11T (=KCTC 13061T =CCUG 53534T =CIP 109363T).", }