RT Journal Article SR Electronic(1) A1 Haouari, Olfa A1 Fardeau, Marie-Laure A1 Cayol, Jean-Luc A1 Casiot, Corinne A1 Elbaz-Poulichet, Françoise A1 Hamdi, Moktar A1 Joseph, Manon A1 Ollivier, BernardYR 2008 T1 Desulfotomaculum hydrothermale sp. nov., a thermophilic sulfate-reducing bacterium isolated from a terrestrial Tunisian hot spring JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 11 SP 2529 OP 2535 DO https://doi.org/10.1099/ijs.0.65339-0 PB Microbiology Society, SN 1466-5034, AB A novel strictly anaerobic, moderately thermophilic, sulfate-reducing bacterium, designated strain Lam5T, was isolated from a hot spring in north-east Tunisia and was characterized phenotypically and phylogenetically. The isolate stained Gram-negative but had a Gram-positive-type cell wall. The strain comprised endospore-forming, slightly curved rod-shaped cells with peritrichous flagella. It did not possess desulfoviridin. Strain Lam5T grew anaerobically at 40–60 °C (optimally at 55 °C) and at pH 5.8–8.2 (optimally at pH 7.1); it did not require NaCl but tolerated concentrations up to 1.5 % (w/v). It utilized lactate, pyruvate, formate, ethanol, butanol, glycerol, propanol and H2 (plus acetate) as electron donors. Lactate was oxidized and pyruvate was fermented to acetate. Sulfate, sulfite, thiosulfate, As(V) and Fe(III) (but not elemental sulfur, fumarate, nitrate or nitrite) were used as electron acceptors. The G+C content of the genomic DNA was 46.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing showed that strain Lam5T was a member of the genus Desulfotomaculum, with Desulfotomaculum putei as its closest relative (96 % similarity to the type strain). On the basis of genotypic, phenotypic and phylogenetic data, strain Lam5T represents a novel species of the genus Desulfotomaculum, for which the name Desulfotomaculum hydrothermale sp. nov. is proposed. The type strain is Lam5T (=DSM 18033T =JCM 13992T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65339-0