@article{mbs:/content/journal/ijsem/10.1099/ijs.0.65364-0, author = "Kevbrin, Vadim and Boltyanskaya, Yulia and Garnova, Elena and Wiegel, Juergen", title = "Anaerobranca zavarzinii sp. nov., an anaerobic, alkalithermophilic bacterium isolated from Kamchatka thermal fields", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2008", volume = "58", number = "6", pages = "1486-1491", doi = "https://doi.org/10.1099/ijs.0.65364-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65364-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "TABS, N-tris (hydroxymethyl)methyl-4-aminobutanesulfonic acid", keywords = "OD, optical density", keywords = "YE, yeast extract", abstract = "A novel obligately anaerobic, alkalithermophilic, chemo-organotrophic bacterium was isolated from a small and very shallow geothermally heated pool at Pushino (Kamchatka, Far East Russia). The bacterium, designated strain JW/VK-KS5YT, was a Gram staining negative, Gram type positive rod. The cells were sometimes branched, with a tendency to grow in long chains, and were non-sporulating and non-motile. The shortest observed doubling time was 28 min when the novel strain was grown at 54–60 °C in 120 mM sodium carbonate-containing medium at pH25 °C 8.5–9.0. The novel bacterium grew on yeast extract and soytone as sole carbon and energy sources but could also use fumarate, thiosulfate and sulfur as electron acceptors. The DNA G+C content was 32.5 mol%. Based on phylogenetic, DNA–DNA hybridization and phenotypic data, it was concluded that isolate JW/VK-KS5YT (=VKM B-2436T=DSM 18970T) represents the type strain of a novel species, Anaerobranca zavarzinii sp. nov.", }