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Volume 58, Issue 3

sp. nov., a moderately halophilic bacterium isolated from a salt lake in China Free

Abstract

A Gram-positive, moderately halophilic, motile, strictly aerobic, endospore-forming, oxidase- and catalase-positive, rod-shaped bacterium, strain YIM kkny16, was isolated from a saline mud sample collected from the Keke salt lake in the Qaidam Basin, north-west China. This isolate grew in the presence of 0–25 % (w/v) NaCl and at pH 6.0–10.0 and 10–50 °C; optimum growth was observed with 10 % (w/v) NaCl and at pH 7.0 and 37 °C. Strain YIM kkny16 had -diaminopimelic acid as the diagnostic diamino acid, MK-7 as the predominant respiratory quinone, with a significant amount of MK-6, and anteiso-C, iso-C and C 7 alcohol as major fatty acids. Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 41.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain YIM kkny16 was a member of the genus , exhibiting sequence similarities of 94.9–97.3 % to the type strains of recognized species. Strain YIM kkny16 could be differentiated from recognized species based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of evidence from this polyphasic study, strain YIM kkny16 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM kkny16 (=DSM 17056=CGMCC 1.6298).

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2008-03-01
2024-04-20
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References

  1. An, S.-Y., Asahara, M., Goto, K., Kasai, H. & Yokota, A.(2007).Virgibacillus halophilus sp. nov., spore-forming bacteria isolated from soil in Japan. Int J Syst Evol Microbiol 57, 1607–1611.[CrossRef] [Google Scholar]
  2. Arahal, D. R., Marquez, M. C., Volcani, B. E., Schleifer, K. H. & Ventosa, A.(1999).Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int J Syst Bacteriol 49, 521–530.[CrossRef] [Google Scholar]
  3. Arahal, D. R., Marquez, M. C., Volcani, B. E., Schleifer, K. H. & Ventosa, A.(2000). Reclassification of Bacillus marismortui as Salibacillus marismortui comb. nov. Int J Syst Evol Microbiol 50, 1501–1503.[CrossRef] [Google Scholar]
  4. Atlas, R. M.(1993).Handbook of Microbiological Media. Edited by L. C. Parks. Boca Raton, FL: CRC Press.
  5. Chen, H.-H., Li, W.-J., Tang, S.-K., Kroppenstedt, R.-M., Stackbrandt, E., Xu, L.-H. & Jiang, C.-L.(2004).Corynebacterium halotolerans sp. nov., isolated from saline soil in the west of China. Int J Syst Evol Microbiol 54, 779–782.[CrossRef] [Google Scholar]
  6. Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef] [Google Scholar]
  7. Cui, X.-L., Mao, P.-H., Zeng, M., Li, W.-J., Zhang, L.-P., Xu, L.-H. & Jiang, C.-L.(2001).Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363. [Google Scholar]
  8. Doetsch, R. N.(1981). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. H. Phillips. Washington, DC: American Society for Microbiology.
  9. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  11. Felsenstein, J.(1993).phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  12. Garabito, M. J., Arahal, D. R., Mellado, E., Marquez, M. C. & Ventosa, A.(1997).Bacillus salexigens sp. nov., a new moderately halophilic Bacillus species. Int J Syst Bacteriol 47, 735–741.[CrossRef] [Google Scholar]
  13. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Nester, E. W., Wood, W. A., Krieg, N. R. & Phillips, G. B. (editors)(1981).Manual of Methods for General Bacteriology. Washington, DC: American Society for Microbiology.
  14. Gregersen, T.(1978). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef] [Google Scholar]
  15. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  16. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  17. Heyndrickx, M., Lebbe, L., Kersters, K., De Vos, P., Forsyth, G. & Logan, N. A.(1998).Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48, 99–106.[CrossRef] [Google Scholar]
  18. Heyndrickx, M., Lebbe, L., Kersters, K., Hoste, B., De Wachter, R., De Vos, P., Forsyth, G. & Logan, N. A.(1999). Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998. Int J Syst Bacteriol 49, 1083–1090.[CrossRef] [Google Scholar]
  19. Heyrman, J., Logan, N. A., Busse, H.-J., Balcaen, A., Lebbe, L., Rodriguez-Diaz, M., Swings, J. & De Vos, P.(2003).Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol 53, 501–511.[CrossRef] [Google Scholar]
  20. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P. & Ward, J. M.(1985). Preparation of chromosomal, plasmid and phage DNA. In Genetic Manipulation of Streptomyces – a Laboratory Manual, pp. 79–80. Edited by D. A. Hopwood, M. J. Bibb, K. F. Chater, T. Kieser, C. J. Bruton, H. M. Kieser, D. J. Lydiate, C. P. Smith, J. M. Ward & H Schrempf. Norwich, UK: F. Crowe & Sons.
  21. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  22. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  23. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  24. Lee, J.-S., Lim, J.-M., Lee, K. C., Lee, J.-C., Park, Y.-H. & Kim, C.-J.(2006).Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. Int J Syst Evol Microbiol 56, 251–257.[CrossRef] [Google Scholar]
  25. Mandel, M. & Marmur, J.(1968). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206. [Google Scholar]
  26. Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef] [Google Scholar]
  27. Proom, H. & Knight, B. C.(1950).Bacillus pantothenticus (n.sp.). J Gen Microbiol 4, 539–541.[CrossRef] [Google Scholar]
  28. Quesada, T., Aguilera, M., Morillo, J. A., Ramos-Cormenzana, A. & Monteoliva-Sánchez, M.(2007).Virgibacillus olivae sp. nov., isolated from waste wash-water from processing of Spanish-style green olives. Int J Syst Evol Microbiol 57, 906–910.[CrossRef] [Google Scholar]
  29. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  30. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  31. Smibert, R. M. & Krieg, N. R.(1981). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  32. Stackebrandt, E. & Ebers, J.(2006). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155. [Google Scholar]
  33. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  34. Stackebrandt, E. & Liesack, W.(1993). Nucleic acids and classification. In Handbook of New Bacterial Systematics, pp. 152–189. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  35. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  36. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  37. Wainø, M., Tindall, B. J., Schumann, P. & Ingvorsen, K.(1999).Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49, 821–831.[CrossRef] [Google Scholar]
  38. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  39. Yoon, J.-H., Oh, T.-K. & Park, Y.-H.(2004). Transfer of Bacillus halodentitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov. Int J Syst Evol Microbiol 54, 2163–2167.[CrossRef] [Google Scholar]
  40. Yoon, J.-H., Kang, S.-J., Lee, S.-Y., Lee, M.-H. & Oh, T.-K.(2005).Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korea. Int J Syst Evol Microbiol 55, 1833–1837.[CrossRef] [Google Scholar]
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Virgibacillus kekensis sp. nov., a moderately halophilic bacterium isolated from a salt lake in China
Int J Syst Evol Microbiol 58, 647 (2008); https://doi.org/10.1099/ijs.0.65365-0
/content/journal/ijsem/10.1099/ijs.0.65365-0
/content/journal/ijsem/10.1099/ijs.0.65365-0
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