1887

Abstract

A Gram-positive, non-spore-forming, rod-shaped, motile bacterium, designated strain DCY22, was isolated from soil of a ginseng field in South Korea and characterized in order to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain DCY22 belonged within the family , and highest levels of sequence similarity were found with 1-19 (96.8 %), ST-26 (96.0 %), ST-50 (95.9 %), ST-74 (95.5 %), PPLB (94.0 %) and DSM 20308 (93.8 %). Chemotaxonomic investigations revealed that strain DCY22 possessed menaquinone MK-9, a common feature of members of the genus . Predominant fatty acids were unknown ECL 13.961 (45.81 %), 17 : 0 anteiso (23.46 %), 18 : 0 iso (15.42 %) and unknown ECL 14.966 (8.70 %). The results of physiological and biochemical tests clearly demonstrated that strain DCY22 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DCY22 (=KCTC 13155=JCM 14841).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65399-0
2008-03-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/3/538.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65399-0&mimeType=html&fmt=ahah

References

  1. Buck, J. D.(1982). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  2. Collins, M. D. & Jones, D.(1981). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45, 316–354. [Google Scholar]
  3. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  4. Fernández-Garayzábal, J. F., Dominguez, L., Pascual, C., Jones, D. & Collins, M. D.(1995). Phenotypic and phylogenetic characterization of some unknown coryneform bacteria isolated from bovine blood and milk: description of Sanguibacter gen. nov. Lett Appl Microbiol 20, 69–75.[CrossRef] [Google Scholar]
  5. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  6. Huang, Y., Dai, X., He, L., Wang, Y. N., Wang, B. J., Liu, Z. & Liu, S. J.(2005).Sanguibacter marinus sp. nov., isolated from coastal sediment. Int J Syst Evol Microbiol 55, 1755–1758.[CrossRef] [Google Scholar]
  7. Kim, M. K., Im, W.-T., Ohta, H., Lee, M. & Lee, S.-T.(2005).Sphingopyxis granuli sp. nov., a β-glucosidase-producing bacterium in the family Sphingomonadaceae in α-4 subclass of the Proteobacteria. J Microbiol 43, 152–157. [Google Scholar]
  8. Kimura, M.(1983).The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  9. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  10. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  11. Montero-Barrientos, M., Rivas, R., Velázquez, E., Monte, E. & Roig, M. G.(2005).Terrabacter terrae sp. nov., a novel actinomycete isolated from soil in Spain. Int J Syst Evol Microbiol 55, 2491–2495.[CrossRef] [Google Scholar]
  12. Pascual, C., Collins, M. D., Grimont, P. A. D., Dominguez, L. & Fernández-Garayzábal, J. F.(1996).Sanguibacter inulinus sp. nov. Int J Syst Bacteriol 46, 811–813.[CrossRef] [Google Scholar]
  13. Prauser, H., Schumann, P., Rainey, F. A., Kroppenstedt, R. M. & Stackebrandt, E.(1997).Terracoccus luteus gen. nov., sp. nov., an ll-diaminopimelic acid-containing coccoid actinomycete from soil. Int J Syst Bacteriol 47, 1218–1224.[CrossRef] [Google Scholar]
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI, Inc.
  16. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H.(1996). Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69. [Google Scholar]
  17. Stackebrandt, E. & Schumann, P.(2000). Description of Bogoriellaceae fam. nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov. and emendation of some families of the suborder Micrococcineae. Int J Syst Evol Microbiol 50, 1279–1285.[CrossRef] [Google Scholar]
  18. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  19. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  20. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J.(1991). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65399-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65399-0
Loading

Data & Media loading...

Supplements

Cellular fatty acid profiles of strain DCY22 and type strains of related species. [PDF](63 KB)

PDF

Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences, showing the phylogenetic relationships between strain DCY22 and related species. [PDF](16 KB)

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error