1887

Abstract

A bacterial strain, ISL-4, comprising Gram-negative, non-motile, rod-shaped cells, was isolated from a marine solar saltern of the Yellow Sea in Korea and was subjected to a polyphasic taxonomic investigation. Phenotypically, the strain resembled members of the genus . It grew optimally at pH 7.0–8.0 and 30 °C and in the presence of 8 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone. The major fatty acids were iso-C and anteiso-C. The DNA G+C content was 37.9 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-4 belonged to the genus , exhibiting sequence similarity values of 95.9–98.6 % with respect to the type strains of recognized species (with the exception of ). Low DNA–DNA relatedness values and differential phenotypic properties, together with its phylogenetic distinctiveness, demonstrated that strain ISL-4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ISL-4 (=KCTC 12975 =CCUG 54354).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65448-0
2008-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/2/365.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65448-0&mimeType=html&fmt=ahah

References

  1. Bowman, J. P.(2000). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868. [Google Scholar]
  2. Bruns, A., Rohde, M. & Berthe-Corti, L.(2001).Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef] [Google Scholar]
  3. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  4. Dobson, S. J., Colwell, R. R., McMeekin, T. A. & Franzmann, P. D.(1993). Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43, 77–83.[CrossRef] [Google Scholar]
  5. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  6. Ivanova, E. P., Bowman, J. P., Christen, R., Zhukova, N. V., Lysenko, A. M., Gorshkova, N. M., Mitik-Dineva, N., Sergeev, A. F. & Mikhailov, V. V.(2006).Salegentibacter flavus sp. nov. Int J Syst Evol Microbiol 56, 583–586.[CrossRef] [Google Scholar]
  7. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  8. Lanyi, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  9. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  10. McCammon, S. A. & Bowman, J. P.(2000). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063.[CrossRef] [Google Scholar]
  11. Nedashkovskaya, O. I., Suzuki, M., Vancanneyt, M., Cleenwerck, I., Zhukova, N. V., Vysotskii, M. V., Mikhailov, V. V. & Swings, J.(2004).Salegentibacter holothuriorum sp. nov., isolated from the edible holothurian Apostichopus japonicus. Int J Syst Evol Microbiol 54, 1107–1110.[CrossRef] [Google Scholar]
  12. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Mikhailov, V. V., Bae, K. S. & Kim, I. S.(2005).Salegentibacter mishustinae sp. nov., isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 55, 235–238.[CrossRef] [Google Scholar]
  13. Nedashkovskaya, O. I., Kim, S. B., Vancanneyt, M., Shin, D. S., Lysenko, A. M., Shevchenko, L. S., Krasokhin, V. B., Mikhailov, V. V., Swings, J. & Bae, K. S.(2006).Salegentibacter agarivorans sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the sponge Artemisina sp. Int J Syst Evol Microbiol 56, 883–887.[CrossRef] [Google Scholar]
  14. Reichenbach, H.(1992). The order Cytophagales. In The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  15. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  16. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  17. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  18. Ying, J.-Y., Liu, Z.-P., Wang, B.-J., Dai, X., Yang, S.-S. & Liu, S.-J.(2007).Salegentibacter catena sp. nov., isolated from sediment of the South China Sea, and emended description of the genus Salegentibacter. Int J Syst Evol Microbiol 57, 219–222.[CrossRef] [Google Scholar]
  19. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef] [Google Scholar]
  20. Yoon, J.-H., Lee, S. T. & Park, Y.-H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef] [Google Scholar]
  21. Yoon, J.-H., Kim, I.-G., Shin, D.-Y., Kang, K. H. & Park, Y.-H.(2003).Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53, 53–57.[CrossRef] [Google Scholar]
  22. Yoon, J.-H., Jung, S.-Y., Kang, S.-J., Jung, Y.-T. & Oh, T.-K.(2007).Salegentibacter salarius sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 57, 2738–2742.[CrossRef] [Google Scholar]
  23. Yurkov, V., Stackebrandt, E., Holmes, A., Fuerst, J. A., Hugenholtz, P., Golecki, J., Gad'on, N., Gorlenko, V. M., Kompantseva, E. I. & Drews, G.(1994). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov. Int J Syst Bacteriol 44, 427–434.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65448-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65448-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error