1887

Abstract

A bacterial strain, designated RS.Sph.026, was isolated from mucus of the coral collected from the northern Red Sea (Gulf of Eilat, Israel). The bacterium was found to be Gram-negative, non-motile, halotolerant and heterotrophic. Comparative 16S rRNA gene sequence analyses showed that strain RS.Sph.026 belonged to the order , with the highest levels of 16S RNA gene sequence similarity with (92 %). Strain RS.Sph.026 grew optimally at a salinity of 3–4 %, pH 7.5–8 and 25–30 °C. The major cellular fatty acids were -7-octadecenoic acid (C 7; 57.2 %) and C cyclo 8 (15.5 %). The DNA GC content of strain RS.Sph.026 was 67.1 mol%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain RS.Sph.026 represents a novel genus and species in the order , for which the name gen. nov., sp. nov. is proposed. The type strain is RS.Sph.026 (=LMG 24307=DSM 19760).

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2008-12-01
2024-03-29
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References

  1. Brown, B. E. & Bythell, J. C.(2005). Perspectives on mucus secretion in reef corals. Mar Ecol Prog Ser 296, 291–309.[CrossRef] [Google Scholar]
  2. Caumette, P., Guyoneaud, R., Duran, R., Cravo-Laureau, C. & Matheron, R.(2007).Rhodobium pfennigii sp. nov., a phototrophic purple non-sulfur bacterium with unusual bacteriochlorophyll a antennae, isolated from a brackish microbial mat on Rangiroa atoll, French Polynesia. Int J Syst Evol Microbiol 57, 1250–1255.[CrossRef] [Google Scholar]
  3. Cho, J. C. & Giovannoni, S. J.(2003).Fulvimarina pelagi gen. nov., sp. nov., a marine bacterium that forms a deep evolutionary lineage of descent in the order ‘Rhizobiales’. Int J Syst Evol Microbiol 53, 1853–1859.[CrossRef] [Google Scholar]
  4. Coffroth, M. A.(1990). Mucous sheet formation on Poritid corals: an evaluation of coral mucus as a nutrient source on reefs. Mar Biol 105, 39–49.[CrossRef] [Google Scholar]
  5. Cooney, R. P., Pantos, O., Le Tissier, M. D. A., Barer, M. R., O'Donnell, A. G. & Bythell, J. C.(2002). Characterisation of the bacterial consortium associated with black band disease in coral using molecular microbiological techniques. Environ Microbiol 4, 401–413.[CrossRef] [Google Scholar]
  6. Denner, E. B. M., Smith, G. W., Busse, H.-J., Schumann, P., Narzt, T., Polson, S. W., Lubitz, W. & Richardson, L. L.(2003).Aurantimonas coralicida gen. nov., sp. nov., the causative agent of white plague type II on Caribbean scleractinian corals. Int J Syst Evol Microbiol 53, 1115–1122.[CrossRef] [Google Scholar]
  7. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  8. Felske, A., Rheims, H., Wolterink, A., Stackebrandt, E. & Akkermans, A. D. L.(1997). Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils. Microbiology 143, 2983–2989.[CrossRef] [Google Scholar]
  9. Frias-Lopez, J., Zerkle, A. L., Boneheyo, G. T. & Fouke, B. W.(2002). Partitioning of bacterial communities between seawater and healthy, black band diseased, and dead coral surfaces. Appl Environ Microbiol 68, 2214–2228.[CrossRef] [Google Scholar]
  10. Garrity, G. M. & Holt, J. G.(2001). The road map to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 119–166. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  11. Giovannoni, S. J. & Rappé, M. S.(2000). Evolution, diversity, and molecular ecology of marine prokaryotes. In Microbial Ecology of the Oceans, pp. 47–84. Edited by D. L. Kirchman. New York: Wiley-Liss.
  12. Hiraishi, A., Urata, K. & Satoh, T.(1995). A new genus of marine budding phototrophic bacteria, Rhodobium gen. nov., which includes Rhodobium orientis sp. nov. and Rhodobium marinum comb. nov. Int J Syst Bacteriol 45, 226–234.[CrossRef] [Google Scholar]
  13. Jannasch, H. W. & Jones, G. E.(1960).Caulobacter in sea water. Limnol Oceanogr 5, 432–433.[CrossRef] [Google Scholar]
  14. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  15. Meikle, P., Richards, G. N. & Yellowlees, D.(1987). Structural determination of the oligosaccharide side-chains from a glycoprotein isolated from the mucus of the coral Acroporaformosa. J Biol Chem 262, 16941–16947. [Google Scholar]
  16. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  17. Rohwer, F., Breitbart, M., Jara, J., Azam, F. & Knowlton, N.(2001). Diversity of bacteria associated with the Caribbean coral Montastrea franksii. Coral Reefs 20, 85–91.[CrossRef] [Google Scholar]
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Shiba, T., Shioi, Y., Takamiya, K. I., Sutton, D. C. & Wilkinson, C. R.(1991). Distribution and physiology of aerobic bacteria containing bacteriochlorophyll a on the east and west coasts of Australia. Appl Environ Microbiol 57, 295–300. [Google Scholar]
  20. Srinivas, T. N. R., Anil Kumar, P., Sasikala, Ch., Ramana, Ch. V. & Imhoff, J. F.(2007).Rhodobium gokarnense sp. nov., a novel phototrophic alphaproteobacterium from a saltern. Int J Syst Evol Microbiol 57, 932–935.[CrossRef] [Google Scholar]
  21. Suzuki, T., Muroga, Y., Takahama, M. & Nishimura, Y.(2000).Roseibium denhamense gen. nov., sp. nov. and Roseibium hamelinense sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from the east and west coasts of Australia. Int J Syst Evol Microbiol 50, 2151–2156.[CrossRef] [Google Scholar]
  22. Wilson, K.(1987). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Green Publishing & Wiley-Interscience.
  23. Zaritsky, A. & Woldringh, C. L.(2003). Localizing cell division in spherical Escherichia coli by nucleoid occlusion. FEMS Microbiol Lett 226, 209–214.[CrossRef] [Google Scholar]
  24. Zaritsky, A., Woldringh, C. L., Fishov, I., Vischer, N. O. E. & Einav, M.(1999). Varying division planes of secondary constrictions in spheroidal Escherichia coli cells. Microbiology 145, 1015–1022.[CrossRef] [Google Scholar]
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