1887

Abstract

A bacterial strain designated GSPC1 was isolated from root nodules of in Segovia (Spain). The 16S rRNA gene sequence of this strain showed 95.9 and 94.7 % sequence similarity, respectively, with those of the type strains of and . Strain GSPC1 presented phenotypic, chemotaxonomic and molecular differences with respect to species which indicated that it belonged to a different species. The isolate was a Gram-positive, aerobic, sporulated rod, motile by means of peritrichous flagella. The strain was catalase-positive and showed weak oxidase activity. It grew in the presence of 2 % NaCl. MK-7 was the predominant menaquinone. anteiso-C, iso-C, iso-C and C were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G+C content was 60.3 mol%. The results of this study suggest that isolate GSPC1 should be classified within a novel species, for which the name sp. nov. is proposed, with strain GSPC1 (=LMG 24086 =DSM 19269) as the type strain.

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2008-08-01
2024-03-28
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J.(1990). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef] [Google Scholar]
  2. Cho, E. A., Lee, J. S., Lee, K. C., Jung, H. C., Pan, J. G. & Pyun, Y. R.(2007).Cohnella laeviribosi sp. nov., isolated from a volcanic pond. Int J Syst Evol Microbiol 57, 2902–2907.[CrossRef] [Google Scholar]
  3. Chun, J. & Goodfellow, M.(1995). A phylogenetic analysis of the genus Nocardia with 16S rRNA sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef] [Google Scholar]
  4. Claus, D. & Berkeley, R. C. W.(1986). Genus Bacillus Cohn 1872. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1105–1140. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  5. Doetsch, R. N.(1981). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington: American Society for Microbiology.
  6. Felsenstein, J.(1983). Parsimony in systematics: biological and statistical issues. Annu Rev Ecol Syst 14, 313–333.[CrossRef] [Google Scholar]
  7. García-Fraile, P., Velázquez, E., Mateos, P. F., Martínez-Molina, E. & Rivas, R.(2006). Phylogenetic study of strains nodulating Phaseolus in Spain on the basis of 16S–23S rDNA intergenic spacer sequences. In Abstracts of the 7th European Nitrogen Fixation Conference, poster 102, 22–27 July 2006, Aarhus, Denmark.
  8. Kämpfer, P., Rosselló-Mora, R., Falsen, E., Busse, H.-J. & Tindall, B. J.(2006).Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov. Int J Syst Evol Microbiol 56, 781–786.[CrossRef] [Google Scholar]
  9. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  10. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  11. Logan, N. A. & Berkeley, R. C. W.(1984). Identification of Bacillus strains using the API system. J Gen Microbiol 130, 1871–1882. [Google Scholar]
  12. Mandel, M. & Marmur, J.(1968). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206. [Google Scholar]
  13. Rivas, R., Velázquez, E., Valverde, A., Mateos, P. F. & Martínez-Molina, E.(2001). A two primers random amplified polymorphic DNA procedure to obtain polymerase chain reaction fingerprints of bacterial species. Electrophoresis 22, 1086–1089.[CrossRef] [Google Scholar]
  14. Rivas, R., Velázquez, E., Willems, A., Vizcaíno, N., Subba-Rao, N. S., Mateos, P. F., Gillis, M., Dazzo, F. B. & Martínez-Molina, E.(2002). A new species of Devosia that forms a unique nitrogen-fixing root nodule symbiosis with the aquatic legume Neptunia natans (L.f.) Druce. Appl Environ Microbiol 68, 5217–5222.[CrossRef] [Google Scholar]
  15. Rosselló-Mora, R. & Amann, R.(2001). The species concept for prokaryotes. FEMS Microbiol Rev 25, 39–67.[CrossRef] [Google Scholar]
  16. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  17. Souza, V., Eguiarte, L., Avila, G., Cappello, R., Gallardo, C., Montoya, J. & Piñero, D.(1994). Genetic structure of Rhizobium etli biovar phaseoli associated with wild and cultivated bean plants (Phaseolus vulgaris and Phaseolus coccineus) in Morelos, Mexico. Appl Environ Microbiol 60, 1260–1268. [Google Scholar]
  18. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  19. Stackebrandt, E., Frederiksen, W., Garrity, G. M., Grimont, P. A., Kämpfer, P., Maiden, M. C., Nesme, X., Rosselló-Mora, R., Swings, J. & other authors(2002). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef] [Google Scholar]
  20. Teng, J. L. L., Woo, P. C. Y., Leung, K. W., Lau, S. K. P., Wong, M. K. M. & Yuen, K. Y.(2003). Pseudobacteraemia in a patient with neutropenic fever caused by a novel paenibacillus species: Paenibacillus hongkongensis sp. nov. Mol Pathol 56, 29–35.[CrossRef] [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  22. Tindall, B. J.(1990). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef] [Google Scholar]
  23. Trujillo, M. E., Kroppenstedt, R. M., Schumann, P. & Martínez-Molina, E.(2006a).Kribbella lupini sp. nov., isolated from the roots of Lupinus angustifolius. Int J Syst Evol Microbiol 56, 407–411.[CrossRef] [Google Scholar]
  24. Trujillo, M. E., Kroppenstedt, R. M., Schumann, P., Carro, L. & Martínez-Molina, E.(2006b).Micromonospora coriariae sp. nov., isolated from the roots of Coriaria myrtifolia. Int J Syst Evol Microbiol 56, 2381–2385.[CrossRef] [Google Scholar]
  25. Velázquez, E., Peix, A., Zurdo-Piñeiro, J. L., Palomo, J. L., Mateos, P. F., Rivas, R., Muñoz-Adelantado, E., Toro, N., García-Benavides, P. & other authors(2005). The coexistence of symbiosis and pathogenicity-determining genes in Rhizobium rhizogenes strains enables them to induce nodules and tumours or hairy roots in plants. Mol Plant Microbe Interact 18, 1325–1332.[CrossRef] [Google Scholar]
  26. Vincent, J. M.(1970). The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of the Root-Nodule Bacteria, pp. 1–13. Edited by J. M. Vincent. Oxford: Blackwell.
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Electron micrographs of strain GSPC1 grown in nutrient agar for 48 h (a), showing flagella, and 72 h (b), showing round spores. Bars, 2.5 µm.

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Electron micrographs of strain GSPC1 grown in nutrient agar for 48 h (a), showing flagella, and 72 h (b), showing round spores. Bars, 2.5 µm.

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Two-dimensional thin-layer chromatogram of polar lipids of strain GSPC1 . DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PL1–PL4, unknown phospholipids; GL1 and GL2, unknown glycolipids; PN, unknown aminophosphoglycolipid.

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